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Southernoregon
Stranger
Registered: 06/04/18
Posts: 5
Last seen: 6 years, 6 months
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Extracting your liquid cultures and purification of alkaloids
#25304803 - 07/02/18 03:59 PM (6 years, 6 months ago) |
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So today I decided to take 3 1 gallon liquid culture jars that were expired and pass them through a funnel. I quickly figured out that even with a very strong vacuum pump mycelial webbing clogged filters really bad I had to go from 5 Micron to 25 micron. I now have a bunch of these little patties after they're dry I will weigh them and see what I can do about extracting them. I have methanol coming in the mail but I have ethanol now and access to a magnetic stirrer and Roto evaporator.

Here is a pic of the "scobe like" patty. I have some in the vacuum oven from last night's first experimental filtration. It is powdery now and after eating a "Finger prints worth" I feel like a wet gram high. So psychoactives are in it.
 Liquid culture was LME and Dextrose. 3 grams of each to each gallon. 15psi for one hour. Filter disk in lid.
After everything dried out and powdery how would you extract it or try to crystallize the extraction or purify it could you use ethanol or is methanol required?
Edited by Southernoregon (07/02/18 04:37 PM)
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Southernoregon
Stranger
Registered: 06/04/18
Posts: 5
Last seen: 6 years, 6 months
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Re: Extracting your liquid cultures and purification of alkaloids [Re: Southernoregon]
#25304920 - 07/02/18 04:51 PM (6 years, 6 months ago) |
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2 hours after eating a fingerprint worth... the high is cleaner and comes with less stomach problems then eating fruits. I'm going to take a portion of this powder and mix it with crystallized CBD and see how it is in capsule form. I'm going to weigh out the rest of the Powder once done and figure out what concentration of ethanol water or even methanol I need to use. This is a for personal use. I know its ridiculous.
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pacmanbreed



Registered: 10/12/16
Posts: 3,829
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Re: Extracting your liquid cultures and purification of alkaloids [Re: Southernoregon]
#25305065 - 07/02/18 06:04 PM (6 years, 6 months ago) |
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Pretty interesting. Ive read from a journal outside shroomeery before they have actives in them..
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Re: Extracting your liquid cultures and purification of alkaloids [Re: pacmanbreed] 1
#25305106 - 07/02/18 06:31 PM (6 years, 6 months ago) |
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Too lazy to look it up but I think erowid has a tek using a 55 gallon drum to grow liquid culture that is then extracted for actives.
That's super cool you're trying this.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Extracting your liquid cultures and purification of alkaloids [Re: elasticaltiger]
#25305256 - 07/02/18 07:53 PM (6 years, 6 months ago) |
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Im curious the yield of mycelium vs fruit bodies. I'm going to wager extraction from mycelium isn't worth it. But still interested in seeing it actually be documented.
Alan has talked about sending plate samples of psilocybe species for scientific analysis and suggesting that even if cops wanted to be dicks it probably wouldn't test positive as it would be below threshold.
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pacmanbreed



Registered: 10/12/16
Posts: 3,829
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Re: Extracting your liquid cultures and purification of alkaloids [Re: bodhisatta]
#25305865 - 07/03/18 05:59 AM (6 years, 6 months ago) |
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 Highly agreed. I really feel they need light and o2 to build those Psilocin molecules in the fruits..
its interesting lc has a slight buzz if its not truly a placebo effect. Then I think it can be compared to a microdose based on the trip report of bein cleaner But in a larger scoby form..
Edited by pacmanbreed (07/03/18 06:04 AM)
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 2 years, 1 month
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Re: Extracting your liquid cultures and purification of alkaloids [Re: pacmanbreed] 1
#25305928 - 07/03/18 07:02 AM (6 years, 6 months ago) |
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If youre making pattys , make a burger and eat it
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pacmanbreed



Registered: 10/12/16
Posts: 3,829
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Re: Extracting your liquid cultures and purification of alkaloids [Re: ManifoldPrime] 1
#25306865 - 07/03/18 05:44 PM (6 years, 6 months ago) |
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Quote:
ManifoldPrime said: If youre making pattys , make a burger and eat it



made me crave for some active vegan burger.
Edited by pacmanbreed (07/03/18 05:50 PM)
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DataVamp
Stranger

Registered: 08/12/15
Posts: 23
Last seen: 6 years, 6 months
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Re: Extracting your liquid cultures and purification of alkaloids [Re: pacmanbreed]
#25307878 - 07/04/18 09:10 AM (6 years, 6 months ago) |
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Love to see how this plays out. How would you know if it's not contaminated though?
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