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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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Offlinegizmodo
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Oops? Well... Maybe?
    #25298804 - 06/29/18 11:30 AM (6 years, 6 months ago)

Good evening, today I inoculated my first sterilized rye berry bag. During the process, I didn't think to inspect the syringe and needle packaging correctly as I thought it would be cut and dry but there was a plastic plug on the end of the syringe and I did not notice so I fumbled around with the syringe for a bit in the process of the prep to inject, opened up the needle and dropped it into the alcohol on accident. I took it out with a soaked gauze pad and flipped it around a second in the pad before I attached it to the syringe... the proper way. LOL. After that I shot a tiny bit of whats inside out of the end to hopefully clear whatever alcohol was in the needle from the dive just in case that would mess things up and I gave the bag a squirt after that..

Wondering if anyone has done that before and if I should be worried about a contamination now? Am I destined to fail after that?

PS - Me when I became Sir fumbles it

Edited by gizmodo (06/29/18 11:34 AM)

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OfflineMrSturgill
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Re: Oops? Well... Maybe? [Re: gizmodo]
    #25299204 - 06/29/18 02:52 PM (6 years, 6 months ago)

If you didn't flame sterilize your needle then inoculate your bag in a SAB dropping it in some alcohol would be the least of your worries.

Without following proper sterile procedure you should be worried about contamination, although you may get lucky, just have to wait and see.


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Read, read, read some more, don't consider anything has been read until everything has been read, then go back and read it again.

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Invisible36fuckin5
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Re: Oops? Well... Maybe? [Re: MrSturgill]
    #25299250 - 06/29/18 03:16 PM (6 years, 6 months ago)

You're probably alright. I'd have flamed it, but it should be fine.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Offlinegizmodo
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Re: Oops? Well... Maybe? [Re: 36fuckin5]
    #25299750 - 06/29/18 08:18 PM (6 years, 6 months ago)

Quote:

36fuckin5 said:
You're probably alright. I'd have flamed it, but it should be fine.




Thank you, I will flame the needles from now on but I hope all is well with the bag. I'll show off the first flush if it goes well.


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InvisibleVonShroomich
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Registered: 06/03/18
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Re: Oops? Well... Maybe? [Re: gizmodo]
    #25300099 - 06/30/18 01:14 AM (6 years, 6 months ago)

I give up on using flame a long time ago. I like to constantly use alcohol to keep everything else clean when I'm injecting. I haven't had an issue just using alcohol. What I do is between each injection, I push out a tiny drop from the needle and wipe it with alcohol. I haven't had any issues with a lot of contamination and I don't use any kind of box for flow hood.

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Invisible36fuckin5
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Re: Oops? Well... Maybe? [Re: VonShroomich]
    #25300324 - 06/30/18 07:13 AM (6 years, 6 months ago)

Quote:

VonShroomich said:
I give up on using flame a long time ago. I like to constantly use alcohol to keep everything else clean when I'm injecting. I haven't had an issue just using alcohol. What I do is between each injection, I push out a tiny drop from the needle and wipe it with alcohol. I haven't had any issues with a lot of contamination and I don't use any kind of box for flow hood.




Fucking awful advice, and your luck will run out soon enough


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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