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pixelpopper
Crap Artist
Registered: 09/20/13
Posts: 4,022
Loc: Dreamland
Last seen: 5 months, 9 days
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Re: How long does your coir remain above 140 degrees? [Re: rockpup] 1
#25280908 - 06/20/18 12:04 PM (5 years, 9 months ago) |
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I actually save my paper towels a lot of the time.
They are just soaked in ISO, so when I am done I just let them sit out or in the SAB or whatever, and they dry out. Then I soak them again next time and reuse
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Kizzle
Misanthrope
Registered: 08/30/11
Posts: 9,866
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Re: How long does your coir remain above 140 degrees? [Re: pixelpopper]
#25282258 - 06/20/18 10:58 PM (5 years, 9 months ago) |
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Quote:
But I suppose bad spawn can be very tricky to see sometimes.
It can be, especially if there's a lot of strongly growing mycelium overwhelming it. Keep an eye out for unusually appearing grains when you shake the jars, sometimes it becomes noticeable then.
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tedoro
ToadStool Tender
Registered: 02/06/15
Posts: 2,212
Last seen: 7 days, 5 hours
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Re: How long does your coir remain above 140 degrees? [Re: Kizzle]
#25315011 - 07/08/18 01:06 PM (5 years, 8 months ago) |
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Thanks everyone for your comments. I've taken some pictures of my recent attempt at bags... maybe you can take a look at it:
https://www.shroomery.org/forums/showflat.php/Number/25315007
Thanks!
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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N2confusion
Stranger
Registered: 02/22/18
Posts: 9
Loc: Deep down in South Missis...
Last seen: 5 years, 6 months
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Re: How long does your coir remain above 140 degrees? [Re: tedoro]
#25322065 - 07/12/18 10:40 AM (5 years, 8 months ago) |
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Hi. Well I just bought a new toy. I bought a new sous vide cooker off Amazon. It is amazing. You can set the temperature and time the length of time it runs. I have some grow bags I bought a few months ago and finally used them. I loaded my substrate into the bag and sealed with a zip tie. I set the temperature for 160 degrees and 4 hours. I let it run at this temperature to pasteurize it. So far the spawn is running through like wild fire. I found this to be a little bit better than trying to watch the stove. It is a pretty good bargain at $70. I did a coir verm cow manure substrate mix brought to field capacity and put in the grow bags. I had previously had a lot of problems with contamination. So I did a whole house sterilization. I added a UV light to my air conditioning unit and I replaced all my duct work. I ran a ozone generator for 6 hours and used a fungicide in a humidifier through the house. I am hopeful that my grows are going to work out from now own. I work on air conditioning equipment so I did all my own work. my old duct work were original from the building of the house. They were turns out the ducts were moldy. There is now new duct board installed. I have one room that I use as my grow room. What i am doing now is to run the ozone generator for 30 minutes before I go in. Ozone kills everything. I also built a laminar flowhood a while back. I know this might be overkill but I am hedging my bets.i also picked up some magnetic covers for my a.c. vents. I put them on before I doing any work in the room. I am not claiming to be an expert an any way. i just started playing around with this hobby 5 months ago. I have been reading a lot of the older more experienced cultivtors tek's. I hope this helps
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: How long does your coir remain above 140 degrees? [Re: N2confusion]
#25322113 - 07/12/18 11:17 AM (5 years, 8 months ago) |
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I would be willing to bet most of us have pretty nasty duct work.
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ManifoldPrime
Per Ardua Ad Astra
Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 3 months
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Re: How long does your coir remain above 140 degrees? [Re: bodhisatta]
#25322191 - 07/12/18 11:58 AM (5 years, 8 months ago) |
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Hey OP, did you still have the issue? because you can verify 100% that your coir prep is the problem by just making extra control boxes with nothing but coir in them and labelling them with the date of the batch of spawn you're bulking. If they trich out at the same time, boom you've confirmed it.
I highly doubt it is the case that your coir prep is the problem, but then again I've seen experienced cultivators mention they switched brands of bricks because they were having higher rates of contams with a specific brand.
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: How long does your coir remain above 140 degrees? [Re: ManifoldPrime]
#25322207 - 07/12/18 12:12 PM (5 years, 8 months ago) |
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Sometimes the spores (syringe, print what have you) are dirty... may be the case of you're certain with your sterile tek.. coir doesn't need, nor will be detrimented by going above 140, it's manure you need to watch temps with because of beneficial microorganisms that sterilization (vs pasteurization) kill off
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pixelpopper
Crap Artist
Registered: 09/20/13
Posts: 4,022
Loc: Dreamland
Last seen: 5 months, 9 days
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Re: How long does your coir remain above 140 degrees? [Re: bodhisatta]
#25322298 - 07/12/18 12:56 PM (5 years, 8 months ago) |
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Quote:
bodhisatta said: I would be willing to bet most of us have pretty nasty duct work.
I definitely, without a doubt have some nasty duct work
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: How long does your coir remain above 140 degrees? [Re: ManifoldPrime]
#25322317 - 07/12/18 01:03 PM (5 years, 8 months ago) |
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Quote:
ManifoldPrime said: Hey OP, did you still have the issue? because you can verify 100% that your coir prep is the problem by just making extra control boxes with nothing but coir in them and labelling them with the date of the batch of spawn you're bulking. If they trich out at the same time, boom you've confirmed it.
I highly doubt it is the case that your coir prep is the problem, but then again I've seen experienced cultivators mention they switched brands of bricks because they were having higher rates of contams with a specific brand.
Another good experiment to determine coir being contamination resistant
Buy a reptile and a terrarium then put bucket tek coir in it (directions are on the package) then see that it takes weeks and weeks before needing replacing even with a living thing shitting on it.
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ManifoldPrime
Per Ardua Ad Astra
Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 3 months
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Re: How long does your coir remain above 140 degrees? [Re: bodhisatta]
#25322399 - 07/12/18 01:47 PM (5 years, 8 months ago) |
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Is that sarcasm
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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elasticaltiger
Like Tigers in Coitus
Registered: 06/24/13
Posts: 8,438
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Re: How long does your coir remain above 140 degrees? [Re: ManifoldPrime]
#25322425 - 07/12/18 02:01 PM (5 years, 8 months ago) |
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I use 180 degree water. It's usually at 135 right at or a little before the 2 hour mark.
Temp at 1 hour ish.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: How long does your coir remain above 140 degrees? [Re: ManifoldPrime]
#25322437 - 07/12/18 02:07 PM (5 years, 8 months ago) |
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Quote:
ManifoldPrime said: Is that sarcasm
No it's what coir is made for... And it makes great reptile bedding because it doesn't go bad even after being hydrated
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: How long does your coir remain above 140 degrees? [Re: bodhisatta]
#25322471 - 07/12/18 02:40 PM (5 years, 8 months ago) |
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I just boil a gallon of water,and mix it in a 5 gallon bucket with a lid, nice rubber gasket... have even boiled half, and added the other half (also boiling hot) an hour later with no issues
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ManifoldPrime
Per Ardua Ad Astra
Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 3 months
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Re: How long does your coir remain above 140 degrees? [Re: bodhisatta]
#25322591 - 07/12/18 03:48 PM (5 years, 8 months ago) |
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Lol I know that. I just suggested that to OP because, like myself, he believes with his eyes and was adamant his problem was his coir. making controls is a scientific way of demonstrating whether your innoculant or medium is the problem. I still make control plates of agar if the batch is over 20 plates.
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: How long does your coir remain above 140 degrees? [Re: ManifoldPrime]
#25322650 - 07/12/18 04:25 PM (5 years, 8 months ago) |
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Quote:
ManifoldPrime said: Lol I know that. I just suggested that to OP because, like myself, he believes with his eyes and was adamant his problem was his coir. making controls is a scientific way of demonstrating whether your innoculant or medium is the problem. I still make control plates of agar if the batch is over 20 plates.
I make control plates too! Lol
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Straight Mush
Mush lover
Registered: 06/08/18
Posts: 511
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Re: How long does your coir remain above 140 degrees? [Re: tedoro]
#25322819 - 07/12/18 06:24 PM (5 years, 8 months ago) |
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Quote:
tedoro said: I've been struggling with contams in my cubes>agar>rye mycobag>coir monotubes . And for the sake of this conversation, lets say its not my rye spawn, but rather the coir that is the problem. (I am confident with my spawn)
I've been doing a double water jacket bucket "improved bucket tek" and have been starting with boiling water, and ending with perfectly hydrated coir well above 140 an hour later. (wrapped in a sleeping bag) With this approach I was getting a one flush, then contams.
Then, in an attempt to make more hydrated coir at one time, I skipped the water jacket, and just put four buckets with coir and water in a sleeping bag and it too stayed above 140f after an hour. Albeit cooler than with a water jacket.
This second, cooler bucket procedure was a disaster. All my tubs contamed within 10 days. All other things were consistent. I'm also using different coir brands to rule that out. Same results.
My gut is saying that my coir prep is at the core of my problem (I am also not very clean when spawning) This forum would say that coir rarely spoils and that my problem is 99% caused by my spawn. I realize this, but for many reasons, i believe it to be my coir. I do not own an oven. How can I best troubleshoot my current situation. Do you have a coir prep experience similar to mine?
Hello sir I haven't read any of the replies but maybe you'll take something from what I have to say aswell.
You should use this Frank's pasteurization tek very easy and makes sure you've pasteurized correctly. Maybe some of the times and temps will help you.
In the summer I only get 2 flushes before I usually contam
-------------------- When in doubt refer to RR's infamous quotes via the search function
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: How long does your coir remain above 140 degrees? [Re: Caps McGee]
#25322877 - 07/12/18 06:57 PM (5 years, 8 months ago) |
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Quote:
Caps McGee said:
Quote:
ManifoldPrime said: Lol I know that. I just suggested that to OP because, like myself, he believes with his eyes and was adamant his problem was his coir. making controls is a scientific way of demonstrating whether your innoculant or medium is the problem. I still make control plates of agar if the batch is over 20 plates.
I make control plates too! Lol
I just consider the fact that not getting contaminants is my control. If you have contamination free plates well...
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: How long does your coir remain above 140 degrees? [Re: bodhisatta]
#25322902 - 07/12/18 07:10 PM (5 years, 8 months ago) |
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Quote:
Straight Mush said:
Hello sir I haven't read any of the replies but maybe you'll take something from what I have to say aswell.
You should use this Frank's pasteurization tek very easy and makes sure you've pasteurized correctly. Maybe some of the times and temps will help you.
In the summer I only get 2 flushes before I usually contam
I think the point was that coir doesn't need pasteurized, manure does... Manure contains microorganisms that are beneficial, and thus pasteurization is ideal, coir doesn't contain these microbes, so full sterilization will suffice... contamination generally points to spawn, unless sub is caught before having a jump on colonization: which in turn, realistically, points to your spawn (if your ratio and water content are on point)
Quote:
bodhisatta said:
Quote:
Caps McGee said:
Quote:
ManifoldPrime said: Lol I know that. I just suggested that to OP because, like myself, he believes with his eyes and was adamant his problem was his coir. making controls is a scientific way of demonstrating whether your innoculant or medium is the problem. I still make control plates of agar if the batch is over 20 plates.
I make control plates too! Lol
I just consider the fact that not getting contaminants is my control. If you have contamination free plates well...
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Kizzle
Misanthrope
Registered: 08/30/11
Posts: 9,866
Last seen: 20 days, 19 hours
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Re: How long does your coir remain above 140 degrees? [Re: Caps McGee]
#25323140 - 07/12/18 09:20 PM (5 years, 8 months ago) |
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Something I noticed is it's actually not all that unusual to find tiny bits of mold during colonization of coir if you really look, especially uncolored molds that turn green or blue on agar. It really got me thinking about the possible connection between strains and contamination. It seems like the times when I had the most problems were also the times where I was testing random isolated strains. Maybe sometimes you just get a strain that can't compete very well.
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: How long does your coir remain above 140 degrees? [Re: Kizzle]
#25323184 - 07/12/18 10:01 PM (5 years, 8 months ago) |
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Quote:
Kizzle said: Something I noticed is it's actually not all that unusual to find tiny bits of mold during colonization of coir if you really look, especially uncolored molds that turn green or blue on agar. It really got me thinking about the possible connection between strains and contamination. It seems like the times when I had the most problems were also the times where I was testing random isolated strains. Maybe sometimes you just get a strain that can't compete very well.
Interesting; not deep enough into my studies to relate... yet! I love this shit!
EDIT: might this suggest there to be strains more resistant? Or do you feel like it maxes out with typical healthy mycelium?
Edited by Caps McGee (07/12/18 10:04 PM)
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