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Invisiblehermanium


Registered: 06/20/10
Posts: 102
Agar Cooling time before MS inoc?
    #25270806 - 06/15/18 07:21 PM (6 years, 7 months ago)

Couldn't find an exact answer to this by search.
How long does Agar in a dish usually take to cool before one can inoculate with MS? I plan to inoculate mine as soon as possible... they're just sitting in the SAB now. B+ and Penis Envy coming UP!

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InvisiblebodhisattaMDiscordReddit
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Re: Agar Cooling time before MS inoc? [Re: hermanium]
    #25270823 - 06/15/18 07:28 PM (6 years, 7 months ago)

as soon as they're cool enough. if you can tell me exactly how hot the agar was going into the dish, your ambient temperature, how high the stacks were, how much volume you poured in to each dish. well shit I still couldn't tell you a time. just check them

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Invisiblehermanium


Registered: 06/20/10
Posts: 102
Re: Agar Cooling time before MS inoc? [Re: bodhisatta]
    #25270852 - 06/15/18 07:43 PM (6 years, 7 months ago)

How do I check without opening them? Tilt them? Should I be concerned the surface temperature will kill the spores?

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OfflineLegend21
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Re: Agar Cooling time before MS inoc? [Re: hermanium]
    #25270858 - 06/15/18 07:46 PM (6 years, 7 months ago)

Quote:

hermanium said:
How do I check without opening them? Tilt them? Should I be concerned the surface temperature will kill the spores?



You should be able to tell just by holding them how warm they are. I’ve only done agar plates a few times but usually for me about an hour or so after they’re cool.

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Invisiblehermanium


Registered: 06/20/10
Posts: 102
Re: Agar Cooling time before MS inoc? [Re: Legend21]
    #25270887 - 06/15/18 08:01 PM (6 years, 7 months ago)

Quote:

Legend21 said:
Quote:

hermanium said:
How do I check without opening them? Tilt them? Should I be concerned the surface temperature will kill the spores?



You should be able to tell just by holding them how warm they are. I’ve only done agar plates a few times but usually for me about an hour or so after they’re cool.




Ok thanks. I will inseminate, I mean inoculate my dishes. :smile:

Edited by hermanium (06/15/18 08:02 PM)

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OfflineNoc
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Re: Agar Cooling time before MS inoc? [Re: bodhisatta]
    #26104973 - 07/12/19 09:06 PM (5 years, 6 months ago)

What temperature are we looking for? Room temp? Dish is still warm?

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InvisibleMateja
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Re: Agar Cooling time before MS inoc? [Re: Noc]
    #26105049 - 07/12/19 09:46 PM (5 years, 6 months ago)

When I pour thin and stack 10 high they are solidified in about 20min, but say 30 to be sure. Also depends a little i guess on the stiffness on the agar. I've been pouring very loose agar lately about 0.7%


And I pour at 45-46C

Quote:

hermanium said:
How long does Agar in a dish usually take to cool



Depends mostly on the temp it's being poured at, thickness of the Puck and if they are stacked. Sometimes if you pour 4-5 stacks at once it will keep the Temps up for a bit longer inside the SAB.


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Edited by Mateja (07/12/19 09:51 PM)

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OfflineSingularFusion
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Re: Agar Cooling time before MS inoc? [Re: Mateja]
    #26105157 - 07/12/19 10:48 PM (5 years, 6 months ago)

yep an hour after pouring I start working on transfers, I also pour around 47°C on the temp gun. I just judge they should not feel warm to my touch when I begin work and then all will be well basically

not very scientific I know, but serving me alright so far

all the best with it

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OfflineSFS96
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Re: Agar Cooling time before MS inoc? [Re: SingularFusion]
    #26105193 - 07/12/19 11:23 PM (5 years, 6 months ago)

I pour at 120f then wait 30 min and start my work:thumbup:


--------------------
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OfflineNoc
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Re: Agar Cooling time before MS inoc? [Re: SFS96]
    #26106035 - 07/13/19 01:05 PM (5 years, 6 months ago)

Quote:

SFS96 said:
I pour at 120f then wait 30 min and start my work:thumbup:




Thanks 30-60 min seems to be the consensus.  Thanks!

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