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 MegaSuperLionheart
Tiger-blooded
Registered: 06/06/18
Posts: 69
Last seen: 2 years, 4 months
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 Preparing for first-time inoculation! 
#25256260 - 06/08/18 05:00 PM (2 years, 7 months ago) |
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Hey, everyone. I already introduced myself over in The Pub, but if you didn't see it... HELLO! My name is Lion.  I really dig this place so far, but let's just get right to the point...
Set up my BRF jars earlier, and they are almost done cooking now! I'll continue to document the journey here, and I hope you guys get to experience your grower's virginity again, vicariously through me! 
Question; I had a bunch of leftover BRF mix. I threw it in a freezer bag, and (obviously) into the freezer. Is it reusable this way? I can't see why not, except for maybe some water loss? I don't know. Has anyone done this before? I have plenty of vermiculite and flour left, so not a big deal if it's a no-go.
 
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MegaSuperLionheart
Tiger-blooded
Registered: 06/06/18
Posts: 69
Last seen: 2 years, 4 months
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Hmm. Thinking about it now, it seems I may have erred. I don't know how I'm going to get the syringe to penetrate that top layer of dry vermiculite, and into the BRF mix. I really don't think it will reach from the holes in the lid. Is this a legitimate concern?
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LtLurker
Lost Sailor
Registered: 01/03/18
Posts: 6,087
Loc: Borderlands 
Last seen: 2 hours, 15 minutes
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Hi and welcome. I've had some low like that before, you should be able to get some liquid to run into the brf mix. Normally you want to fill up to where the threads start.
For saving your mix, IDK. I always just toss the little extra after a make because it'll get moldy quick. Never tried to freeze it though.
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MegaSuperLionheart
Tiger-blooded
Registered: 06/06/18
Posts: 69
Last seen: 2 years, 4 months
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 Re: Preparing for first-time inoculation! [Re: LtLurker]
#25256568 - 06/08/18 07:16 PM (2 years, 7 months ago) |
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That's what I'm hoping for. My assumption is the mycelium will find the sustenance of the BRF, and adjust accordingly. Then again, this is a blind assumption.
We'll see how it plays out. Thanks for the reassurance!
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MegaSuperLionheart
Tiger-blooded
Registered: 06/06/18
Posts: 69
Last seen: 2 years, 4 months
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Hello, everyone. I just want to make an eventless update on my situation. It's been three days, and of course, no activity yet. It's my understanding that it could be 7-14 days before I see any signs of colonization. So, I'm really not worried yet, but I am having some doubts.
This goes back to the concerns I had a few days ago, earlier in this thread. Although I did reach some of the actual BRF mix with my inoculations, they were mostly in the dry vermiculite layer on top. Will this negatively impact
the grow? Will mycelium begin colonization in dry vermiculite? I'm sure if it does, then it will make its way to the BRF mix.
I know I'm probably just being impatient and worrisome, and that I should just observe and see how it plays out. However, I can't help it. lol I have BIG plans for these mushrooms.
Does anyone have experience with this?
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LtLurker
Lost Sailor
Registered: 01/03/18
Posts: 6,087
Loc: Borderlands
Last seen: 2 hours, 15 minutes
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Be patient. Can take a few days to see signs of germination. Every where you saw liquid run when you injected should have spores in it.
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 Ziran
The Hero of Time
Registered: 02/03/16
Posts: 5,954
Loc: Temple of Time
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Re: Preparing for first-time inoculation! [Re: LtLurker]
#25261778 - 06/11/18 05:10 PM (2 years, 7 months ago) |
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Quote:
Ziran said:
And into the jar the needle goes. Try to angle the needle a bit, so when you slide it in it goes up against the glass in between the verm layer and the glass. I use about a drop or two in each inoculation hole
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Song Of Healing
 Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.
 
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MegaSuperLionheart
Tiger-blooded
Registered: 06/06/18
Posts: 69
Last seen: 2 years, 4 months
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Re: Preparing for first-time inoculation! [Re: Ziran]
#25261794 - 06/11/18 05:18 PM (2 years, 7 months ago) |
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Great! That's the technique I used. I suppose I'm just worried about it getting hung up on the dry vermiculite. Haha, I realize I am being quite impatient. It's just very exciting. xD
Thank you, gentlemen. Cheers!
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Cjmckay
Registered: 04/09/18
Posts: 378
Last seen: 3 months, 29 days
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I could see mine flow down the glass and to the bottom. I'm pretty heavy handed with the syringe though. I didn't blend the rice so well this time, kinda chunky.
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MegaSuperLionheart
Tiger-blooded
Registered: 06/06/18
Posts: 69
Last seen: 2 years, 4 months
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Re: Preparing for first-time inoculation! [Re: Cjmckay]
#25262224 - 06/11/18 08:50 PM (2 years, 7 months ago) |
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Not me, unfortunately. T_T
I split one syringe (10 ml) between 6 jars, 4 inoculations each. Some ran down. Most didn't. :/
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Cjmckay
Registered: 04/09/18
Posts: 378
Last seen: 3 months, 29 days
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Quote:
MegaSuperLionheart said:
Not me, unfortunately. T_T
I split one syringe (10 ml) between 6 jars, 4 inoculations each. Some ran down. Most didn't. :/
That should be more than enough juice to get them rolling.
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MegaSuperLionheart
Tiger-blooded
Registered: 06/06/18
Posts: 69
Last seen: 2 years, 4 months
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Re: Preparing for first-time inoculation! [Re: Cjmckay]
#25262278 - 06/11/18 09:13 PM (2 years, 7 months ago) |
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I hope so!
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Cjmckay
Registered: 04/09/18
Posts: 378
Last seen: 3 months, 29 days
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Getting fuzzy where the stream ran down.
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Thatguy52
Stranger
Registered: 06/02/18
Posts: 3
Last seen: 2 years, 4 months
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Re: Preparing for first-time inoculation! [Re: Cjmckay]
#25268147 - 06/14/18 05:33 PM (2 years, 7 months ago) |
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Beginner myself... How long was it from inoculation until you saw some fuzz grow?
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Nighted
Ghost
Registered: 11/10/01
Posts: 698
Loc: Funkytown
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Quote:
Question; I had a bunch of leftover BRF mix. I threw it in a freezer bag, and (obviously) into the freezer. Is it reusable this way? I can't see why not, except for maybe some water loss? I don't know. Has anyone done this before? I have plenty of vermiculite and flour left, so not a big deal if it's a no-go.
Yes, it will be fine in the freezer.
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Freedom defined is freedom denied.
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Nighted
Ghost
Registered: 11/10/01
Posts: 698
Loc: Funkytown
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Re: Preparing for first-time inoculation! [Re: Cjmckay]
#25268179 - 06/14/18 05:44 PM (2 years, 7 months ago) |
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Quote:
I didn't blend the rice so well this time, kinda chunky.
It will be fine the way it looks now. Don't be concerned about it at all. 
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Freedom defined is freedom denied.
Nighted is better than Google. Please take time to rate 5/5. Thanks!
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Cjmckay
Registered: 04/09/18
Posts: 378
Last seen: 3 months, 29 days
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Re: Preparing for first-time inoculation! [Re: Thatguy52]
#25268288 - 06/14/18 06:30 PM (2 years, 7 months ago) |
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That was about 4 days. Seems to be going a lot faster than my first round though the temps were just starting to warm up then and now it's pretty warm.. These cakes are a lot less packed too. Some of the rice is still chunky. I made these with my own print/syringe.
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Cjmckay
Registered: 04/09/18
Posts: 378
Last seen: 3 months, 29 days
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Re: Preparing for first-time inoculation! [Re: Nighted]
#25268303 - 06/14/18 06:36 PM (2 years, 7 months ago) |
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Yeah these seem to really be moving quick. A lot more porous than the fine flour cakes.
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MegaSuperLionheart
Tiger-blooded
Registered: 06/06/18
Posts: 69
Last seen: 2 years, 4 months
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Re: Preparing for first-time inoculation! [Re: Cjmckay]
#25268308 - 06/14/18 06:38 PM (2 years, 7 months ago) |
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UPDATE: It's been six days now, and... BAM! First signs of mycelium growth!
Of the six jars, only one remains unchanged. The rest are all showing activity now! I apologize for the shitty camera angles, but I really didn't wanna pick them up or disturb them, so... These three show the most.
  
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Opticz
Registered: 06/07/18
Posts: 199
Loc: Matrix
Last seen: 2 years, 5 months
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I pick mine up all the time and look all around them lol
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