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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Re: At what point do you give up on a tub pinning/fruiting? [Re: JHOVA]
#25253901 - 06/07/18 10:22 AM (5 years, 8 months ago) |
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Quote:
LtLurker said: Trying to find a better picture, but does it look like Mycogone? Grows blobby and gets yellow spots like that.
https://www.shroomery.org/forums/showflat.php/Number/23252350#23252350 https://www.shroomery.org/forums/showflat.php/Number/23424292#23424292
Here's a good pic. From here

Think we have a winner. (Props to you for spotting that, I couldn't ID that shit)
It being a white mold would make sense as to why I didn't spot it on a petri; must have been hiding in a very uniform ring or something, weird.
One thing though; I've been giving this thing TONS of FAE and Moisture. It only just developed the yellow droplets and it's still fucking colonizing like normal mycelium. This is the weirdest shit I've seen in terms of a contam.
I disposed of a large portion of those plates and have all new tubs/LC's now, so I doubt my current plates have this issue (may be a good thing I tossed that petri out)
Still, it doesn't explain the first tub, which was made five days prior.
Maybe I got lazy with my inoculation the second time? I have to tell you, I highly doubt this happened during the LC Biopsy; so I'm assuming it was already on the plate from my first transfers, and I had no way of knowing being new to agar and white molds.
Still, it smells of healthy mushroom...
LOL
Like literally REEKS of mushroom.
Maybe a flowhood isn't a total waste of money after all.
Edited by plurfekt (06/07/18 10:39 AM)
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LtLurker
Lost Sailor



Registered: 01/03/18
Posts: 7,535
Loc: Borderlands
Last seen: 16 days, 13 hours
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Re: At what point do you give up on a tub pinning/fruiting? [Re: plurfekt] 1
#25254015 - 06/07/18 11:27 AM (5 years, 8 months ago) |
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I hope you can work it out and get back to clean growin. A couple people got this a few months back. They said the same thing about looking & smelling like mushies up until the blobs came, if I remember right. Sounds hard to spot.
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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Re: At what point do you give up on a tub pinning/fruiting? [Re: LtLurker]
#25254047 - 06/07/18 11:44 AM (5 years, 8 months ago) |
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Quote:
LtLurker said: I hope you can work it out and get back to clean growin. A couple people got this a few months back. They said the same thing about looking & smelling like mushies up until the blobs came, if I remember right. Sounds hard to spot.
That tub is currently soaking in a bleach solution.

Really wish I'd known sooner as I fanned and misted it for a couple fuckin' weeks in my fruiting room.
I looked and none of my current cultures came from that plate, but now I have to be extra wary of my other plates being clean.
Outcome: Need flowhood & pour agar for better visibility to spot things like this. I looked at my plates of promise under lights for a considerable amount of time without spotting anything, so obviously need to work on that.
This thread: https://www.shroomery.org/forums/showflat.php/Number/22020260
Is about isolating hidden contaminants on agar (got the link from the other link)
And I'll be putting that into practice heavily after this...
I left the other tub alive, waiting for it to do something. No pins in a week or so and it's dead. I don't want slow genetics and I have a shitload of spawn coming from new plates/different cultures.
I'm going to call this the Achilles Heel of Pasty Plates, which I have a great deal of respect for.
Lack of visibility making this kind of contamination nearly impossible to spot, then again, I doubt it would be easy on regular petri's either - but easier!
Edited by plurfekt (06/07/18 12:34 PM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: At what point do you give up on a tub pinning/fruiting? [Re: plurfekt] 1
#25254124 - 06/07/18 12:26 PM (5 years, 8 months ago) |
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Yeah glad someone said mycogone. I was over here like yeah 100% mycogone. Yeah it smells like shrooms and can even bruise blue if it's colonizing over cube myc. It's a tricky bitch to spot for sure and easily could have been riding along all along.
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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Re: At what point do you give up on a tub pinning/fruiting? [Re: Mad Season]
#25254140 - 06/07/18 12:37 PM (5 years, 8 months ago) |
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Quote:
Mad Season said: Yeah glad someone said mycogone. I was over here like yeah 100% mycogone. Yeah it smells like shrooms and can even bruise blue if it's colonizing over cube myc. It's a tricky bitch to spot for sure and easily could have been riding along all along.
Your thread is the precedent apparently :P
Must not be very common? I've been into myco 10 years, never seen anything like this and I've made plenty of mistakes in plenty of different locations.
Had to be my very first agar runs too...a bit salty over that, lmao. I had good flushes from the same MS PE syringe that made these petris up in the first place using nothing but sugar.
+1 to you for knowing your shit; this is the type of thing that only comes from experience.
OH FOR OTHERS SUSPECTING THIS TYPE OF CONTAMINATION: Immediately I noticed a difference in how it was colonizing. It appeared very aggressive but at the same time tomentose - at first. For a brief moment prior to the mycogone displaying itself it became highly rhizomorphic, perhaps a response to the contamination.
The "give away" I know now is that on the sides of your colonized sub, those bumps will display before they do on top during colonization. Maybe not every time, but I remember watching the sides of the tub thinking "what kind of crack is this culture on..." & then waiting for blobs to develop, which I've grown over a pound of.
Anyway, maybe that will help someone, maybe not.
Thanks for all the answers/help guys. My best input is really just don't fuck around with your sterile work even if you think things are all clean and good, slight mistakes can cause huge consequences. Luckily I didn't lose a penny in all of this.
New tubs and cultures inbound 
WE LIVE TO FIGHT ANOTHER DAY!
ALSO... MYCOGONE CAN BE ID'ED VIA MICROSCOPE.
SO I'M FUCKIN' GETTIN' ONE OF THOSE!

I find this very informational and decided to add it for people who run into mycogone contamination, also I killed the second tub just for safe measure - it hadn't fruited in a month anyway - I don't want slow cultures even on the off-chance. I still have to test remaining LC's, plates, etc for this contamination as it's basically impossible to see in a pasty plate to an untrained eye. Literally, they all look clean, and I feel like I'd need them in actual petri's under a microscope to know.
Mycogone. This fungal disease is transmitted primarily by contaminated casing. It infects the pins or mushroom initials as they start to form but symptoms do not appear for 10-14 days. As you say - by then it is too late. The best defense is to make sure the casing is well steamed prior to application to the beds. If necessary, treatment of the casing with 1% formalin can be used if this is legal where you are growing. Treatment of the casing with benomyl, carbendazim, prochloraz manganese or thiabendazole can also be effective. If this is a chronic problem on the farm, I suggest application of these fungicides as a preventative to the casing at every crop. There has been no reported resistance to the benzimadazole fungicides but rotation of these chemicals is always a good practice if possible. Many times Mycogone is seasonal so if you have a season of the year (typically drier) when Mycogone is not so prevalent you can suspend the fungicide treatments during those months. Sanitation and hygiene is of utmost importance and must be part of farm management year round. . If you find diseased mushrooms, weeping caps, distorted shapes, infected stipes - these mushrooms must be picked off by your best pickers wearing gloves before watering or harvest and disposed of off the farm. Watering is an important way the spores of this fungus are spread. Infected casing / compost should be isolated with heavy applications of salt or by pushing a plastic pot deep into the compost casing. Steaming after the crop has been harvested is very important to eliminate this threat from the farm. The spores can become air-borne so this must be done before cleanout.
Edited by plurfekt (06/10/18 06:34 AM)
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