Never success with Monotubs - maybe bacteria contamination? - Mushroom Cultivation

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apshroom
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Registered: 10/27/17
Posts: 9
Last seen: 5 years, 9 months

Never success with Monotubs - maybe bacteria contamination?

#25197020 - 05/11/18 05:58 AM (5 years, 11 months ago)

Hello dear Shroomery,
have been reading here very often and intensive. Have also build a flowhood after the input from here and doing some layman like mycology for some months.

I tried several Monotubs, but all without success - should be around 10 to 12 fails...
I think i first didn't really realize or understand the procedure, so here a quick summary how i do:

A spore syringe with Ko Samui was the beginning

Inoculated via port to BRF cakes and had some mushrooms

Transferred the mycelium from the inner side to agar agar plates, first i bought them, now i make them with malt, the growth is very fast and seems to be clean and homogen always. Have made maybe 50 transfers in the flowhood, always without problems of contamination. Please see pics

Then is use organic rye and verm for filling my jars after the Roger Rabbit video. I use 0,5 liter jars with 2 holes and tyvek fixated with 1 micropore above it. Rye is soaking for minimum 24 hours after washing. Then i add some gypsum in the soaking water. cooking it up for 10 mins and let all the steam go out. testing the field capacity on toilet paper

Then sterilization of the jars by pressure cooking and for some hot air autoclave from hospital

Agar transfer in the flowhood after cooling down over night - disinfection of scalpel and tweezers with flame after every step. That was on 8 of March

in the winter i had put them in my incubator, small fridge with a thermostat + ceramic heat bulb in 26-28 degrees celsius. They incubated in 2-4 weeks. Now in the summer i use just a black plastic box on top of the shelf and they incubate in the same time, maybe faster...

Then i want to grow bulk:

Bucket Tek with a small cooling box with coco coir bricks and verm and hot water + one monotub ( first try ) with pasteurized horse poo. I used organic horse poo pellets, watered them a little and pasteurized them via Roger Rabbit video way

So there were 3 smaller tubs (30liter) and one bigger for the poo(60liter)

Monotubs have 4 holes, 2 on level of substrate and 2 up on the smaller sides

Taped all holes with 2 layers micropore and duct tape

Lined the inside with disinfected aluminum foil, nice in the corners and sprayed everything out with alcohol - let it dry

Then layer after layer substrate and bulk - all clean with gloves

No i casing layer with verm or stuff like that, i tried this and oyster gritt earlier but also without success and i think i realized that cubensis dont need top layer
But i put a cling film under the lid, because my boxes are not complete air tight

Put them on my shelf and wait... that was on 26 of March and 28 of March on the bigger poo tub

Colonized not too fast but on 14 of April i removed the duct tape on the first tubs, without one i kept closed for about a week more. This one contaminated on the surface 2 weeks later...

So now im waiting since about one month for something in my tubs, but nothing happens...
No contaminations but also no pinning, there are nice drops on the inside of the boxes and some on the substrate, it looks just nice white to me. I moved them to a window faced north and opened it a little bit for fresh air exchange

Have i started fresh air exchange to early or is maybe my spawn contaminated?
It does smell like fungi when i opened the jars but also some sweet taste. Is that the problem?
In the actual jars i made 31 of March there are some jars that dont fully colonize, but most are developing fine i hope...

Is there some big mistake in my procedure? I just ask, because my first attempts to monotubs had some fundamental mistakes in timing and fresh air exchange.

Maybe someone can help me

Alex

Monotub #1

Monotub #2

Monotub #3 Horse poo

Extras:

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