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Clumpy spore syringes......whats the deal??
    #2522558 -

I just received a bunch of syringes from a place that I have never done business with, initials "E.T."; only one of the syringes seemed to be perfectly distributed sporewise, the rest had one single clump of spores in each syringe. These single clumped syringes even after 5-10 minutes of vigorous shaking still had smaller clumps of spores as well- I mean, the shaking definitly helped but there were still some small clumps that I just had to go ahead and shoot into the cakes.... The spores were Creepers and Golden Teachers...I have read contradictory reports on this, but I would like to hear something from actual growers. Thanks......


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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2522571 -

I have another question I would like to ask the experts..... After innoculating each site using cakes, how important is wiping the syringe down with alcohol? I have my suspicions with this, like what if tiny fibers from the paper towel go into the jars? Also, how dangerous is it for the needle to not be totally dry before innoculating the next site? Thanks a lot guys...


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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2522606 -

Just about every grow guide will mention shaking your syringes violently to break up the spores inside. The ones i recieved from sporesource didnt have any clumps, but i still shook them for about 2 minutes(until my arm was tired). I've even read about one guy who made hit own syringe shaker. just keep shaking until broken up. If the syringes were sitting for a long time, then the clumps are normal. oh ya...when you're shaking, make sure to suck up a bit of air into the syringe, so there's an air bubble inside, to help break up the spores.


I think it's very important to wipe the syringe after each innoculation. You dont want to cross-contaminate your jars. in other words...if your PC or boiling didnt kill all of the contamination in one of your jars, then not wiping the syringe down will transfer the contams to the next jar, and so on.

I dont think you'll have to worry about little fibers from the paper towel causing a problem. the towel had alcohol on it, so contams should be killed at that point. by the way...i used a cotton ball, not a paper towel.

i didn't wait for the syringe to be totally dry before innoculating the next site. I just innoculated about 3 days ago, placed my jars in an incubator, and am now seeing mycelium growing in all of my B+ jars. Nothing in the Equadorian strains is visible yet though.


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(and no, that's not me in the avatar)
Yahoo! Pacific Northwest Mycology Group

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Re: Clumpy spore syringes......whats the deal?? [Re: mycoguy]
    #2522685 -

What about taking a lighter to the needle in between jars? The alcohol gets most of the black residue left from the lighter, but can any remaining residue hurt my cakes?


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Re: Clumpy spore syringes......whats the deal?? [Re: mycoguy]
    #2522693 -

Quote:
mycoguy said:oh ya...when you're shaking, make sure to suck up a bit of air into the syringe, so there's an air bubble inside, to help break up the spores.





If you do this though you want to make sure you suck the air into the syringe while holding the tip in a lighter flame,drawing the air though the flame.Otherwise you may very well be drawing contamination right into your syringe before you even start.The rest of what Mycoguy saidm though is right on. :thumbup:

and nope that little bit of carbon build upp wont hurt anything,because when you wipe it with alchahol,its steralized.

Edited by snatchcakes (04/03/04 07:04 PM)

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Re: Clumpy spore syringes......whats the deal?? [Re: snatchcakes]
    #2522723 -

Thanks to both of you.......

hey, this is kind of off-topic but, I am using a heating pad to keep my cakes warm, (sunbeam king size), and I wasn't sure of which setting to leave it on, low-med-high, I switched it to high like a day after innoculation, went to check on it and the jars were hot man, I quickly lowered the setting...They weren't hot enough to burn the under side of my wrist, but they were pretty hot....what do you guys think of this? whats the likelyhood of damaging the spores? Dniceoner ~~~~~~~~~~


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Edited by DNICEONER (04/03/04 07:09 PM)

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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2522741 -

turn it on low and place a towel between the heating pad and the jars.Ideal temp is 86 degrees.Lower than that and they are fine they just take longer,higher than 86 and you will kill the mycelluim or the spores.Do a search on heating pads.There have been several threads recently about them.

if the jars are warm to the touch,its too hot!

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Re: Clumpy spore syringes......whats the deal?? [Re: snatchcakes]
    #2522781 -

dammmmmn, they were more than warm.......are they dead or what? its day 3 and I can't see any mycellium really....


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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2522854 -

well no not nescarly dead,and sometimes it may take a week or so before you see anything,just lower the heat and be patient.They are probably going to take a little longer using a heating pad anyway so just try and relax.take a thermometer and place it soome water thats 86 degrees,its not hot at all.
probably you caught it in time,but i recomend you invest in a tub in tub incubator.theres a thread now where you can make one for less than $20.It makes all the differance in the world and takes alot of the worrying away.

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Re: Clumpy spore syringes......whats the deal?? [Re: snatchcakes]
    #2522870 -

I've done this a couple of times before, this is just the first time I've used a heating pad, and also the first time I have used pre-sterilized jars that I purchased online from E.T....... Now that I think of it, when inocculating my jars they seemed kind of dry, like when pulling the needle out, the cake rose with it......What do you think about this? I don't remember that happening when I prepared my own......


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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2523093 -

DNICEONER:

i had the same situation with major spore clumping in a syringe. the supplier assured me the spores were still good, and they were. the clumping effect may happen when spores are scraped off the print paper, and some paper tissue is scraped off with it into the solution.

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Re: Clumpy spore syringes......whats the deal?? [Re: taibensis]
    #2523130 -

You know, in one of the syringes attatched to a spore clump was this whitish mass, I don't even know how to describe it other than like what a little piece of mycellium would look like inside the plunger of the syringe immersed in water and all....... Has anyone seen this? Is that even possible?


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Edited by DNICEONER (04/03/04 09:19 PM)

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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2523195 -

yes some of the spores probably started to germainate inside the syringe.Not a problem,infact may help to speed up colonization time some.

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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2523657 -

Tape it to a vibrator and turn it on...it works...


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WARNING chronicshroom will rip you off! Don't trade with him! I sent him 20 spore syringes and he never sent me anything.

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Re: Clumpy spore syringes......whats the deal?? [Re: kb73]
    #2526176 -

Yeah I don't have a vibrator bro and neither does my woman, but I can't knock you for your sexual orientation. Do you....


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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2526193 -

Hardy har har...it works....you ain't the first person to ask this, and it ain't the first time myself or someone else has suggested the same thing...


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WARNING chronicshroom will rip you off! Don't trade with him! I sent him 20 spore syringes and he never sent me anything.

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Re: Clumpy spore syringes......whats the deal?? [Re: snatchcakes]
    #2526310 -

snatchcakes said:
turn it on low and place a towel between the heating pad and the jars.Ideal temp is 86 degrees.Lower than that and they are fine they just take longer,higher than 86 and you will kill the mycelluim or the spores.Do a search on heating pads.There have been several threads recently about them.
if the jars are warm to the touch,its too hot!


woah woah woah.  cubensis thermal death occurs at like 106 degreed F.  the mycelium just wont grow as fast at 87 degrees as it would 86.  the same goes for 85 and 86.

peace  :spliff:


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Farewell FRSE!  We had such good times together.

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Re: Clumpy spore syringes......whats the deal?? [Re: acidhead1279]
    #2526318 -

Your right about thermal death man, let me ask you something Acid, what, in your opinion, would 106 degree feel like on a PF jar.....would it burn the underside of your wrist? can you compare it to something?


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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2526344 -

honestly no experience with that. but personaly i would say that yout jars PROBABLY didnt get that hot, i could be wrong of coarse. :crazy:

my suggestion would be to get a box and put your jars inside that.  then set your pad underneath it or wrap an electric blanket around it.


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Farewell FRSE!  We had such good times together.

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Re: Clumpy spore syringes......whats the deal?? [Re: DNICEONER]
    #2526356 -

DNICEONER said:
I just received a bunch of syringes from a place that I have never done business with, initials "E.T.


http://www.shroomery.org/vendors.php

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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