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OfflineMycillyum
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Reanimation of dry tissue culture.
    #25216858 - 05/20/18 05:53 PM (2 years, 11 months ago)

So first off I am as new as they come in relation to cultivating mushrooms.
To rectify this I have been on these forums for months reading in the background coming up on knowledge and processes. I took the advices of TC's and others providing good information. So I immediately started with agar watched all of RR's videos and practice very good sterile techniques. Moving forward I am doing everthing I can to improve my abilities. This may seem a bit out of my reach but I am doing everything success or fail to gain practical usable knowledge. A few weeks back I heard the idea of bringing mycelium "back from the dead" from dried tissue. So a buddy of mine brought over some Blue Meanies to eat and I had the idea to try and get the culture going again. I took some dried stem pieces and placed them on MEA. I waited, expecting contamination it was just an experiment. Then what I think was mycelium started to grow. I remember RR stated in his experience it took 4-6 weeks to see growth from dried tissue. So here are some pics and maybe the community can weigh in on what they think.



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InvisiblebodhisattaM
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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25216884 - 05/20/18 06:08 PM (2 years, 11 months ago)

the last two plates are the only ones I would use. they actually look good. not only promising but good


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OfflineMycillyum
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Re: Reanimation of dry tissue culture. [Re: bodhisatta]
    #25216912 - 05/20/18 06:22 PM (2 years, 11 months ago)

Damn! One of the first posts and Bodhi chimes in! I am actually using your easy AF teks in my procedures right now. Thats great man, the contaminated plates are the first ones I dropped stems onto, the last two are the plates I transferred away from contamination. I'm just unsure if the cuture is re animated because it seems really fast based on what I've read so far. It looks like it is so far though.


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InvisibleHumble Newcomer
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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25216913 - 05/20/18 06:23 PM (2 years, 11 months ago)

Do you know if these were freshly dried?


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InvisiblebodhisattaM
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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25216918 - 05/20/18 06:24 PM (2 years, 11 months ago)

those last two plates are clearly mycelium and you definitely got it working.
I'm actually really surprised but glad you did it.


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OfflineMycillyum
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Re: Reanimation of dry tissue culture. [Re: Humble Newcomer]
    #25216921 - 05/20/18 06:25 PM (2 years, 11 months ago)

I reckon within the past few weeks or so.


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OfflineDr_Smegma
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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25216970 - 05/20/18 06:44 PM (2 years, 11 months ago)

This is super cool. I'm also interested in how long these have been dried for, a few days? weeks? or longer?

I have always heard you can get spores from dried out fruits but I didn't know you could clone them as well.


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OfflineMycillyum
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Re: Reanimation of dry tissue culture. [Re: bodhisatta]
    #25216998 - 05/20/18 06:58 PM (2 years, 11 months ago)

Man so am I! I was expecting failure right away, but I figured for the sake of experimenting and learning something new I had plates ready so why not?


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OfflineThedenthead
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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25217002 - 05/20/18 07:00 PM (2 years, 11 months ago)

Makes me wanna try it too.


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OfflineMycillyum
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Re: Reanimation of dry tissue culture. [Re: Thedenthead]
    #25217034 - 05/20/18 07:16 PM (2 years, 11 months ago)

Just do it! All you have to lose is a few dishes. I hope to put the mycelium to grain soon and grow it out.


Edited by Mycillyum (05/20/18 07:17 PM)


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OfflineMycillyum
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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25246960 - 06/03/18 10:24 PM (2 years, 10 months ago)

Here is that BM culture thats been transferred.


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Offlineverum subsequentis
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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25246992 - 06/03/18 10:50 PM (2 years, 10 months ago)

i've done this before with success too. good job. i absolutely agree with bodhi on the last two being the keepers but i really like the last one. i think the second to last has something else growing in it. I'd make a couple of transfers out of the last one and then put the best looking ones to grain.


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Re: Reanimation of dry tissue culture. [Re: verum subsequentis]
    #25247004 - 06/03/18 11:05 PM (2 years, 10 months ago)

Yeah I wonder how long the dried specimens would be viable for after drying.  I wonder if someone who bought a bag on the street could make up agar plates and just put a little in there.  Cool experiement, great idea.


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Offlineverum subsequentis
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Re: Reanimation of dry tissue culture. [Re: naturalistic123]
    #25247037 - 06/03/18 11:24 PM (2 years, 10 months ago)

mine were dried upon harvesting and then stored in a mason jar for a year. they came back to life on agar.


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Re: Reanimation of dry tissue culture. [Re: verum subsequentis]
    #25247045 - 06/03/18 11:29 PM (2 years, 10 months ago)

That's fucking nuts.  Magic mushrooms.


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Re: Reanimation of dry tissue culture. [Re: verum subsequentis]
    #25247115 - 06/04/18 12:18 AM (2 years, 10 months ago)

Yea that's what I did. It grew some funky shit as expected. Transfered away looks clean now.


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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25249936 - 06/05/18 01:38 PM (2 years, 10 months ago)

Transferred BM pin coming back to life also took the area the pin came from. BM and PESH for the next grow while cloning the best Burma has to offer this go round.


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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25249966 - 06/05/18 01:57 PM (2 years, 10 months ago)

Love this.


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Re: Reanimation of dry tissue culture. [Re: elasticaltiger]
    #25250016 - 06/05/18 02:43 PM (2 years, 10 months ago)

:grin: its been fun this very short while I've been practicing this hobby.


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Invisibleamy1193
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Re: Reanimation of dry tissue culture. [Re: Mycillyum]
    #25250027 - 06/05/18 02:50 PM (2 years, 10 months ago)

Very cool


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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