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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlinerockpup
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Registered: 03/02/18
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Thicker Verm layer on top?
    #25116725 - 04/05/18 07:16 PM (5 years, 10 months ago)

I think I need to use more verm layer on top of my cakes?
I been using about 1"- I do not want to go more as the needle may not reach the depth it needs to be.Some thing is infecting my jars-I am not sure if something is coming in contact with my needle before I inject.

I do not wipe, I just flame with a alchol burner, maybe my burner is leaving dirty residue?The needles pre-packed sterile- Should  I wipe then flame then wipe? I figure wiping after is against the tek rules.
It does seem to be coming from the injection sites.

I am trying again saturday or friday-the jars been sitting 4-5 days-in sanitized clean plastic bin.I hope there is no growth, if so I know it not my injecting.


--------------------
SKATE OR DIE!
RIP 1989-2001-The best of time's
I miss molly.

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InvisiblebodhisattaMDiscordReddit
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Re: Thicker Verm layer on top? [Re: rockpup]
    #25119160 - 04/06/18 05:52 PM (5 years, 10 months ago)

Its in your syringe then.

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OfflineDayLaborer
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Re: Thicker Verm layer on top? [Re: bodhisatta]
    #25119822 - 04/06/18 11:49 PM (5 years, 10 months ago)

Do you wipe the inside lip of the jar before adding the dry verm layer?

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Offlinerockpup
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Re: Thicker Verm layer on top? [Re: DayLaborer]
    #25120946 - 04/07/18 02:08 PM (5 years, 10 months ago)

Yeah I wipe man! From back to front, don't want no stuff in my V jj.No I did wipe-made sure dry.I used a canning funnel also-I liked it-worked well, less clean up.

OK I did 11 more-No signs of infection at all before injection(day6).I went crazy sterile this time-Full suit/booties/gloves/hairnet/hair covering/-3m chemical respirator-Showered right before-Cleaned everything with lysol and Cavicide.

Made a air spray with tea tree oil/witch hazel and distilled water-sprayed clean air room down-I only supply air into room with hepa filtered air.which is off during work.Plus the insane fumes from cleaning should keep everything safe.

OTHER than a flow hood I could not get any more sterile.


--------------------
SKATE OR DIE!
RIP 1989-2001-The best of time's
I miss molly.

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OfflineDayLaborer
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Posts: 113
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Re: Thicker Verm layer on top? [Re: rockpup]
    #25121903 - 04/07/18 09:08 PM (5 years, 10 months ago)

Its typical to get a few that are contaminated each run.

Sounds like you should be good this time around though.

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InvisiblebodhisattaMDiscordReddit
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Re: Thicker Verm layer on top? [Re: rockpup]
    #25122440 - 04/08/18 06:10 AM (5 years, 10 months ago)

Quote:

rockpup said:
Yeah I wipe man! From back to front, don't want no stuff in my V jj.No I did wipe-made sure dry.I used a canning funnel also-I liked it-worked well, less clean up.

OK I did 11 more-No signs of infection at all before injection(day6).I went crazy sterile this time-Full suit/booties/gloves/hairnet/hair covering/-3m chemical respirator-Showered right before-Cleaned everything with lysol and Cavicide.

Made a air spray with tea tree oil/witch hazel and distilled water-sprayed clean air room down-I only supply air into room with hepa filtered air.which is off during work.Plus the insane fumes from cleaning should keep everything safe.

OTHER than a flow hood I could not get any more sterile.



Did you use a still air box?
Your tea tree spray is absolutely useless.
Filtering air into a room is also useless, as soon as you walk in its no longer filtered air in there humans make millions of particles a minute.

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Offlinerockpup
Old skater punk
I'm a teapot User Gallery

Registered: 03/02/18
Posts: 312
Loc: Everywhere or anywhere
Last seen: 4 years, 4 months
Re: Thicker Verm layer on top? [Re: bodhisatta]
    #25185138 - 05/05/18 12:21 AM (5 years, 9 months ago)

Been using a still air box and a rack under now-I like the idea of keeping up off a larger area for contamantion to land.Had to take the rack out to do my grain jars.


--------------------
SKATE OR DIE!
RIP 1989-2001-The best of time's
I miss molly.

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