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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
would this work?
    #2518456 - 04/02/04 11:28 AM (12 years, 8 months ago)

Could I 'sterilize' a syringe by sucking up some very weak h2o2 solution, leaving a few mins and then triple rinsing with sterile water...?

In Dr Rush's new book, he is using weak peroxided agar plates for germinating spores (I think the theory is that a) the h2o2 content is REAL low and b) the spores are dropped on in 'clumps' so that the massive amount of spores generating overcomes the h2o2)

I have a feeling that the funky neon syringes I got from the 100 Yen shop might not survive anything heat related...


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Shrooms aren't everyone's cup of tea... (Some folks just eat 'em)


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InvisibleJohn
ssdp.org

Registered: 08/08/03
Posts: 7,026
Loc: Vancouver, B.C.
Re: would this work? [Re: sakura]
    #2518591 - 04/02/04 12:33 PM (12 years, 8 months ago)

peroxide won't sterlize it just stops spores from germinating hence use it on pre sterlized agar, contam spores won't germinate but mush spores will becuase there's so many.

suck a little water in the syringe and put it in the microwave for like 2mins it should survive, take the needle off, if you can't do that use bleach, but wash it out a lot with sterlized (boiled water) :stoned:


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There's a thin line between sanity and insanity... and I just snorted it.


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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
Re: would this work? [Re: John]
    #2520028 - 04/02/04 09:51 PM (12 years, 8 months ago)

Hmmm... Might have to go the bleach route (The needles can't be removed)

Here's a pic of them.


--------------------
Shrooms aren't everyone's cup of tea... (Some folks just eat 'em)


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Invisibledobinky
infoseeker
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Registered: 01/30/03
Posts: 2,436
Loc: Wiki, Kentucky
Re: would this work? [Re: sakura]
    #2520031 - 04/02/04 09:52 PM (12 years, 8 months ago)

afoaf always just gets boiling water...sucks it up 4 or 5 times and then fills it once more and voila...sterile syringes


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Today?s Pig is Tomorrow?s Bacon


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Invisiblesimplemachine
Manfly
Male User Gallery

Registered: 09/14/03
Posts: 1,981
Re: would this work? [Re: sakura]
    #2520056 - 04/02/04 10:12 PM (12 years, 8 months ago)

^^^ thats what I reccomend, too. suck up some rubbing alcohol first if your not convinced. after 2-3 rinses (suck in and squirt out after sitting for 10 min) it should be pretty darn clean.


sM


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Invisibledobinky
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Registered: 01/30/03
Posts: 2,436
Loc: Wiki, Kentucky
Re: would this work? [Re: simplemachine]
    #2520058 - 04/02/04 10:13 PM (12 years, 8 months ago)

and dont squirt it back into the main pan..


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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