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OfflineMlp275
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Idea for making a LC syringe from prints... Will this work?
    #25168002 - 04/26/18 09:59 PM (6 years, 8 months ago)

I have a few prints on the way and I wanted to make liquid cultures from them. Rather than wasting a jar when I only need enough LC for a couple rye bags, I was wondering if this idea would work...

Follow a standard spore syringe procedure

Except replace the sterile water with sterilized solution of 1tsp honey/100ml.

This way the LC will grow directly in the syringe, eliminating the need for a jar.

Will this work?

Edited by Mlp275 (04/26/18 10:00 PM)

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OfflineGrimsweeper
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Re: Idea for making a LC syringe from prints... Will this work? [Re: Mlp275]
    #25168010 - 04/26/18 10:03 PM (6 years, 8 months ago)

Spores are dirty by nature. The consensus is to go spore to agar, clean agar to LC.


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Invisibleelasticaltiger
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Re: Idea for making a LC syringe from prints... Will this work? [Re: Grimsweeper] * 1
    #25168046 - 04/26/18 10:22 PM (6 years, 8 months ago)

Don't make lc from spores :facepalm:

If you do this you should make the print from an invitro grow and print it directly in a sterilized glass jar. Then you can just squirt some sterile water in, swish it around suck up the solution into your lc syringe.

So if you're using an lc syringe you should save 5ml of space for 5ml of water you're going to use for swishing the spores around.


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