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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 526
Last seen: 4 months, 4 days
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Ziploc twist and seal PP5 containers are not air/water tight!!!
#25163740 - 04/25/18 04:27 AM (6 years, 8 months ago) |
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So I had a very bacterial outbreak when i switched to the PP5 ziploc containers (unmodified) I was very upset and tried to figure out how everything went so wrong. I never had any failure rates with the age old glass jars with tyvek covered lids. So as I was washing all my ziploc containers I decided to see how much of a deal these babys actually have I filled one up with water twisted the lid on and turned upside down thinking that I would have to squeeze the bottle a bit to get water to pour out but nope just tipping it over water poured out of these bottles! So this must be why I got so much bacterial contams my damn bottles are not fully sealed units and with no filtration they allowed anything to come in.
Now when I tried this with a new ziploc bottle it was sealed water tight. So I can only assume once these babies get PC'ed they must shrink (bottle) or expand (lid) thus no longer making them air water tight sealed.
Questions:
Should I just say screw these bottles and go back to the glass jars?
Could I use cling plastic wrap around the lids like how one would wrap up a agar dish and would this plastic wrap prevent contams from getting inside?
Or should I cover the bottle with tyvek then place lid on top. It totally works (lid screws down nice and tight) but this would then have the grain inside bottle make contact with tyvek when shaking not sure if this contact would moisten the tyvek and cause contams to get through
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ShroomyToons
Be Calm and Grow On



Registered: 01/16/17 
Posts: 3,867
Loc: Rebel Flag
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Re: Ziploc twist and seal PP5 containers are not air/water tight!!! [Re: more4u2c]
#25163751 - 04/25/18 04:41 AM (6 years, 8 months ago) |
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I'd probably just go back to the glass. But if the tyvek allows you to tighten the lid sufficiently, that could work, too.
It's good you found what was going on. Gets very frustrating when lids are problematic.
--------------------
 
"Never miss a good chance to shut up"
Best advice ever...in this hobby
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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 526
Last seen: 4 months, 4 days
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Re: Ziploc twist and seal PP5 containers are not air/water tight!!! [Re: ShroomyToons]
#25163782 - 04/25/18 05:14 AM (6 years, 8 months ago) |
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I just love these PP5 platic bottles I really badly want them to work lol. The small ones are perfect for agar work and the fact that they are all plastic it makes them light weight, stackable so when not being used they take up little space being plastic they dont rust up like the glass jar lids. So many positives over the glass jars but the one negative that truly matters is making these babies contam resistant (puts on thinking cap)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Ziploc twist and seal PP5 containers are not air/water tight!!! [Re: more4u2c]
#25164048 - 04/25/18 08:34 AM (6 years, 8 months ago) |
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Find another plastic bottle that will work. Search up polypropylene bottles.
But this is why I've never been for unmodified lids. You need a filter.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Ziploc twist and seal PP5 containers are not air/water tight!!! [Re: 36fuckin5]
#25164089 - 04/25/18 08:57 AM (6 years, 8 months ago) |
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Tupperware is for leftovers imo
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