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thelanzii

Registered: 11/13/12
Posts: 5,435
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AA+ Agar>Grain Shoebox Grow Along
#25145757 - 04/17/18 02:06 PM (6 years, 8 months ago) |
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This is my first time doing agar. I have grown in the past with ms>grain and have had ok success
I just inoculated 6 plates and here was my experience
I will post pics when the plates have more growth
the plate style was a kerr wide mouth half pint with a plastic lid un modified
i had a few difficulties but overall i think it went well
I found it really hard to break up the spore clumps in the syringe, i tried very hard not to suck air back up into the syringe and was barely able to break the clumps up, I imagine there is a technique to this but couldn't find much in the search results
another thing i found difficult was opening up the lids from the alcohol coated jars
i used an alcohol coated towel as grip which was still difficult to open them but got the job done. the towel hit the floor of the sab a few times which i wasn't thrilled about
i plan on doing pasty plates in the future but i already have these jars poured and have seen other people successful with them around here
tips and suggestions much appreciated
really hoping for a successful grow this time around

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mr_dude
Wanderer


Registered: 03/02/16
Posts: 93
Loc: Mordor
Last seen: 4 years, 9 months
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Re: AA+ Agar>Grain Shoebox Grow Along [Re: thelanzii] 1
#25145777 - 04/17/18 02:17 PM (6 years, 8 months ago) |
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i too struggled with agar work in a sab. just curious though, why jars and not petris? feels like they are trickier to work with. ive never personally had problems with clumping syringes, i just shake the shit out of it. sometimes the plunger gets harder to push but with a slight more pressure it comes out fine. i wish u the best man
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thelanzii

Registered: 11/13/12
Posts: 5,435
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Re: AA+ Agar>Grain Shoebox Grow Along [Re: mr_dude]
#25145807 - 04/17/18 02:41 PM (6 years, 8 months ago) |
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i didnt want to pour and liked that i could reuse the jars
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: AA+ Agar>Grain Shoebox Grow Along [Re: thelanzii]
#25145875 - 04/17/18 03:21 PM (6 years, 8 months ago) |
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I ise those exact jars and love them. Super visibility compared to glad minis.
Leave the lids barely screwed on when pcing. Then when the pressure drops to zero remove every agar jar. Get a roll of saran wrap and buy a pvc cutter from home depot. Cut a 1.5 -2.0 inch section and wrap the edge twice. If not mold has formed on unopened plates. If you notice in 1 or 2 days there is excessive moisture open them in a sab on their side flick the ecxess water on the towel or sab floor gently.
Josex has a nice video demonstratig good transfer technique in his no pour tek. Try emulatizng that so you can feel confident you wont drop or fuck up a much sought after clean culture.
To minimize excess moisture from SS squirt some solution on a freshly charred xacto knife once to cool it and twice to have it drip. What remians as surface tensioj can be hsed to make a gentle slice down the middle without a fuckin mess everywhere.
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Edited by JHOVA (04/17/18 03:24 PM)
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thelanzii

Registered: 11/13/12
Posts: 5,435
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Re: AA+ Agar>Grain Shoebox Grow Along [Re: JHOVA]
#25147279 - 04/18/18 04:08 AM (6 years, 8 months ago) |
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so you never screw back on the tops after pcing?
why do you keep the lids so loose? i had them about half an inch loosened
i was planning on getting them slightly loosened before i put them in my sab
not sure what you mean about mold
i looked up how to shake a syringe and found that people suck air back in through a flame and then are able to shake better which is a nice tip
i picked up 16 pasty plates today and plan on working with them as well
might just make some pf-tek jars out of these to ensure i have some more spores if things go south
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: AA+ Agar>Grain Shoebox Grow Along [Re: thelanzii]
#25147393 - 04/18/18 06:26 AM (6 years, 8 months ago) |
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You keep the plastic lids loose then tighten them directly after the pc cycle and apply cling wrap. Otherwise ots impossiboe to open them with a vaccum seal in the sab.
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thelanzii

Registered: 11/13/12
Posts: 5,435
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Re: AA+ Agar>Grain Shoebox Grow Along [Re: JHOVA]
#25147658 - 04/18/18 09:52 AM (6 years, 8 months ago) |
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inoculated 6 more plates today
plan on transferring to 14 pasty plates and then tiger dropping
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thelanzii

Registered: 11/13/12
Posts: 5,435
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Re: AA+ Agar>Grain Shoebox Grow Along [Re: thelanzii]
#25162992 - 04/24/18 06:23 PM (6 years, 8 months ago) |
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whats this growth look like
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