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OfflineAmanitus
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This is not healthy mycelium, is it? with PICS
    #25157206 - 04/22/18 08:59 AM (6 years, 8 months ago)

Hello shroomerites.

I got these jars/tumblers that were inoculated 10 days ago and some of them have fine stringy mycelium growing and others have been growing a kind of "fog" that rapidly spread all over the place. In some cases went through the dry verm layer and also trying to go through the lid :eek:







Is this trichoderma?

I had a jar start growing proper mycelium and then went into "foggy" mode and some jars had just fog from the start.

How do you avoid the fog? Was my PC sterilization bad? Was it the syringe or were the jars "attacked" once they were resting in the shelves?

Thanks so much.

Edited by Amanitus (04/22/18 05:42 PM)

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Offlinemushhiehunter
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Re: This is not healthy mycelium, is it? with PICS [Re: Amanitus]
    #25157216 - 04/22/18 09:04 AM (6 years, 8 months ago)

That's either a very agressive mycelial growth or you will see it turn green very soon. I'm no expert on this but it seems fine to me.. the only startling thing is the speed (10 days to this stage is a bit suspicious)

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Invisibleelasticaltiger
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Re: This is not healthy mycelium, is it? with PICS [Re: mushhiehunter]
    #25157280 - 04/22/18 09:40 AM (6 years, 8 months ago)

What kind of filter do you have on the jars / glasses. Is there a hole in the foil on top?

First picture definitely doesnt look kosher.

2nd and 3rd also doesn't look good byt its hard to tell if it's from environmental factors or contamination. They might be okay.

You can avoid this kinda thing by using real mason jars with a dry verm layer and 4 holes in the lids for gas exchange.

Did you inject your spores on the sides or in the middle?


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OfflineKKVguitarist
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Re: This is not healthy mycelium, is it? with PICS [Re: elasticaltiger]
    #25157312 - 04/22/18 09:57 AM (6 years, 8 months ago)

Doesn't look good. Maybe birth in som plastic bags. Give fresh air 4 or 5 times a day and the second you see something wrong toss it.

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OfflineAmanitus
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Re: This is not healthy mycelium, is it? with PICS [Re: elasticaltiger]
    #25157799 - 04/22/18 01:53 PM (6 years, 8 months ago)

Quote:

elasticaltiger said:
What kind of filter do you have on the jars / glasses. Is there a hole in the foil on top?




The mason jar is a whole brown rice cake with no dry verm layer on top. The filter is micropore tape i covered the inoculation holes with. I have 5 of those wbr jars, only one has has that white fog thing.

The glass tumblers are verm/brf pf tek with a dry verm layer on top.

Quote:

You can avoid this kinda thing by using real mason jars with a dry verm layer and 4 holes in the lids for gas exchange.




I know. I just can't find mason jars in my country. The ones i have, i bought once i travelled to the US. That's why i'm using glass tumblers with a lid made of foil. I tried plastic pp5 containers but they suck ass. They get shitloads of condensation after i PC them.

Quote:

Did you inject your spores on the sides or in the middle?




I injected solution on the sides. That's why i think pics 2 and 3 are fucked  up as well. Because the white fog thingy did not show up only near inoculation points but all over the place. If it were mycelium it would grow near inoculation points, right?

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InvisibleZiran
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Re: This is not healthy mycelium, is it? with PICS [Re: Amanitus]
    #25157873 - 04/22/18 02:28 PM (6 years, 8 months ago)

Quote:

Amanitus said:
Quote:

elasticaltiger said:
What kind of filter do you have on the jars / glasses. Is there a hole in the foil on top?




The mason jar is a whole brown rice cake with no dry verm layer on top. The filter is micropore tape i covered the inoculation holes with. I have 5 of those wbr jars, only one has has that white fog thing.

The glass tumblers are verm/brf pf tek with a dry verm layer on top.

Quote:

You can avoid this kinda thing by using real mason jars with a dry verm layer and 4 holes in the lids for gas exchange.




I know. I just can't find mason jars in my country. The ones i have, i bought once i travelled to the US. That's why i'm using glass tumblers with a lid made of foil. I tried plastic pp5 containers but they suck ass. They get shitloads of condensation after i PC them.

Quote:

Did you inject your spores on the sides or in the middle?




I injected solution on the sides. That's why i think pics 2 and 3 are fucked  up as well. Because the white fog thingy did not show up only near inoculation points but all over the place. If it were mycelium it would grow near inoculation points, right?




pftek doesnt need MP tape. Just a dry verm layer. if you dont wanna use a dry verm layer you need a filter (sfd, tyvek, poly, MP tape) on the lid during the PC cycle, and a SHIP (self healing injection port)


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OfflinehamloafM
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Re: This is not healthy mycelium, is it? with PICS [Re: Ziran]
    #25157919 - 04/22/18 02:56 PM (6 years, 8 months ago)

With a pressure cooker, SAB, and grain jar lids you could go completely unmodded on the lids, and open jars to inoculate inside of sterile conditions.


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InvisibleZiran
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Re: This is not healthy mycelium, is it? with PICS [Re: hamloaf]
    #25157942 - 04/22/18 03:04 PM (6 years, 8 months ago)

Quote:

hamloaf said:
With a pressure cooker, SAB, and grain jar lids you could go completely unmodded on the lids, and open jars to inoculate inside of sterile conditions.




this will work too.


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.


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OfflineAmanitus
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Re: This is not healthy mycelium, is it? with PICS [Re: Ziran]
    #25158283 - 04/22/18 05:29 PM (6 years, 8 months ago)

Quote:

Ziran said:
pftek doesnt need MP tape. Just a dry verm layer. if you dont wanna use a dry verm layer you need a filter (sfd, tyvek, poly, MP tape) on the lid during the PC cycle, and a SHIP (self healing injection port)




Two of those 3 "infested" jars actually have a dry verm layer.

Edited by Amanitus (04/22/18 05:30 PM)

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Offlinehongomagico
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Re: This is not healthy mycelium, is it? with PICS [Re: Amanitus]
    #25158406 - 04/22/18 06:37 PM (6 years, 8 months ago)

that growths is spotty is suspicious too. only spots should grow from inoculation sites not from everywhere,,

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OfflineAmanitus
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Re: This is not healthy mycelium, is it? with PICS [Re: hongomagico]
    #25161986 - 04/24/18 10:15 AM (6 years, 8 months ago)

You guys have made me think and i have a new question now.

If i have PF TEK jars that have no dry verm layer but have micropore tape over the inoculation holes, is that enough for a filter and GE?

Or is just a layer of micropore tape over 4 holes a weak filter for airborne attacks AND a weak form of GE?

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Offlinemushhiehunter
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Re: This is not healthy mycelium, is it? with PICS [Re: Amanitus]
    #25162126 - 04/24/18 11:17 AM (6 years, 8 months ago)

Quote:

Amanitus said:
You guys have made me think and i have a new question now.

If i have PF TEK jars that have no dry verm layer but have micropore tape over the inoculation holes, is that enough for a filter and GE?

Or is just a layer of micropore tape over 4 holes a weak filter for airborne attacks AND a weak form of GE?





micropore is not good enough, I think you found the culprit yourself :smile:

The dry verm layer serves as a really good filter in pf tek (unless disturbed).

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OfflineAmanitus
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Re: This is not healthy mycelium, is it? with PICS [Re: mushhiehunter]
    #25162512 - 04/24/18 02:11 PM (6 years, 8 months ago)

Quote:

mushhiehunter said:
Quote:

Amanitus said:
You guys have made me think and i have a new question now.

If i have PF TEK jars that have no dry verm layer but have micropore tape over the inoculation holes, is that enough for a filter and GE?

Or is just a layer of micropore tape over 4 holes a weak filter for airborne attacks AND a weak form of GE?





micropore is not good enough, I think you found the culprit yourself :smile:

The dry verm layer serves as a really good filter in pf tek (unless disturbed).




What is fucking with my mind is the fact that both jars with the dry verm layer and jars without it got infested with that white yogurt thing.

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