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InvisibleMrRabbit-HMR
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Hydrating pre-STERALIZED straw
    #25161478 - 04/24/18 02:12 AM (6 years, 8 months ago)

I've recently been given a number of bales of pre-steralized chopped straw (used as horse bedding for fancy horses).

https://www.cavierabedding.co.uk/caviera_products/raviera-pro/

I know the best way to prepare straw is to pasteurize it, but this comes pre-steralized so I'm looking for the best way to hydrate it and spawn it.

I was thinking of creating a liquid inoculant from a number of grain jars, then mixing that into the soak water. Then after the straw has been soaked, I would mix in the grain.

Hopefully this would help increase the speed of colonization which I assume is key considering I wont have any beneficial bacteria to help fight my corner until colonization is complete?

Any tips or suggestions?

Thanks

Edited by MrRabbit-HMR (04/24/18 03:44 AM)

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OfflineP.Avgvst
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Re: Hydrating pre-STERALIZED straw [Re: MrRabbit-HMR]
    #25161740 - 04/24/18 07:09 AM (6 years, 8 months ago)

Maybe you could find just a handful of untreated straw and mix in. It would work as a “starter”. Then, maybe after a week or so incubating it, you could pasteurize everything and then inoculate the mycelium .

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InvisibleMrRabbit-HMR
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Re: Hydrating pre-STERALIZED straw [Re: P.Avgvst]
    #25161780 - 04/24/18 08:02 AM (6 years, 8 months ago)

Really? A part of me doesn't think that would work as the straw is already very dry...

I've never worked with steralized straw before, so I'm thinking the chances of contamination are high, yet I've read here that people have used it with success.

Anyone have any experience with steralized straw?

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Re: Hydrating pre-STERALIZED straw [Re: MrRabbit-HMR]
    #25161823 - 04/24/18 08:30 AM (6 years, 8 months ago)

Josex, pasty, eat and steevo know their straw. Maybe try them.


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InvisiblebodhisattaMDiscordReddit
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Re: Hydrating pre-STERALIZED straw [Re: JHOVA]
    #25161827 - 04/24/18 08:35 AM (6 years, 8 months ago)

I would get it wet and pasteurize it

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OfflinehamloafM
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Re: Hydrating pre-STERALIZED straw [Re: MrRabbit-HMR]
    #25161830 - 04/24/18 08:39 AM (6 years, 8 months ago)

Quote:

MrRabbit-HMR said:
Really? A part of me doesn't think that would work as the straw is already very dry...

I've never worked with steralized straw before, so I'm thinking the chances of contamination are high, yet I've read here that people have used it with success.

Anyone have any experience with steralized straw?



Do you have any pictures of said straw for an example of it's hydration level? 

If it's sterilzed, as long as your sterile conditions are good it won't become contaminated unless it touches open air.  You are correct over dry straw being a bigger vector for contamination in open air substrates utilizing pasteurized wheat straw.  This is because when straw dries out, and is rehydrated, any trich spores that land upon it will germinate faster than mushroom mycelium can recover and take over said rehydrated straw.


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Re: Hydrating pre-STERALIZED straw [Re: hamloaf]
    #25161841 - 04/24/18 08:47 AM (6 years, 8 months ago)

Sterilized straw is like a sterilized pallet. At some point it was heat treated. Its no longer sterile. Its not in impervious packaging

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Re: Hydrating pre-STERALIZED straw [Re: bodhisatta]
    #25161844 - 04/24/18 08:50 AM (6 years, 8 months ago)

Yea, I know all that.  Your suggestion is a good one though.


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InvisibleMrRabbit-HMR
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Re: Hydrating pre-STERALIZED straw [Re: hamloaf]
    #25161874 - 04/24/18 09:17 AM (6 years, 8 months ago)

I'm not sure I'm understanding why wetting it and pasteurizing it is going to help?

As it's sterilized already, all the beneficial bacteria has been killed along with the mold spores/trich spores etc, so what benefit is there to wetting it and pasteurizing it?

I'm probably missing something here, it just doesn't make sense to me.

The straw is currently around 10% hydrated.

Because of the amount I have, I was looking at growing some oysters on it also, probably in columns suspended in my fruiting room. Therefore it will be spawned in open air.

This is the straw:



It was my thinking that if I could find a way to speed up the mycelium colonization of the straw, perhaps with some kind of liquid inoculant mixed in whilst soaking the straw, might give the mycelium a good enough headstart to outrun any contaminants? < might be a terrible/pointless idea?

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Re: Hydrating pre-STERALIZED straw [Re: MrRabbit-HMR]
    #25161900 - 04/24/18 09:31 AM (6 years, 8 months ago)

As soon as you take it out of the bag and before you start to pasteurize it its not sterilized straw anymore.
Just pretend its normal straw lol

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Re: Hydrating pre-STERALIZED straw [Re: bodhisatta]
    #25161908 - 04/24/18 09:35 AM (6 years, 8 months ago)

When exposed to the air, it will be contaminated with fungus spores and (maybe?) with some good bacteria. So, if you let them grow and then pasteurize, you may end with a “normal” straw. No idea if it is a good suggestion or not, maybe someone could give a better idea.

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Re: Hydrating pre-STERALIZED straw [Re: P.Avgvst]
    #25161920 - 04/24/18 09:40 AM (6 years, 8 months ago)

Not much microbes live on stocks of wheat straw.  No where near as much as manure.  Bods suggestion of simply pasteurizing straw really is a good one.


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InvisibleMrRabbit-HMR
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Re: Hydrating pre-STERALIZED straw [Re: P.Avgvst]
    #25161936 - 04/24/18 09:45 AM (6 years, 8 months ago)

Quote:

bodhisatta said:
As soon as you take it out of the bag and before you start to pasteurize it its not sterilized straw anymore.
Just pretend its normal straw lol




Yeah, understood.

Is there any point pasteurizing it then in that case or would a cold soak suffice just to rehydrate?

Would you care to guess what the contam rate would be in percentage if it was just cold soaked? I could live with a <20% likelihood of contamination?

Finally, if a cold soak is OK, would there be any benefit in colonization time if I were to soak the straw in a weak liquid inoculant solution?

Quote:

P.Avgvst said:
When exposed to the air, it will be contaminated with fungus spores and (maybe?) with some good bacteria. So, if you let them grow and then pasteurize, you may end with a “normal” straw. No idea if it is a good suggestion or not, maybe someone could give a better idea.




Is that how it works? Does the good bacteria come from air-born sources? I always just assumed it came from the ground as the straw grew.

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Re: Hydrating pre-STERALIZED straw [Re: MrRabbit-HMR]
    #25161961 - 04/24/18 10:01 AM (6 years, 8 months ago)

soaking step is for mere hydration of wheat straw.  After being hydrated straw will still need a pasteurization bath to clean it, so myce will have a window of opportunity to colonize it without being competed with.


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Edited by hamloaf (04/24/18 10:09 AM)

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