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Some Guys Fungi
Your Highness



Registered: 04/17/18
Posts: 7
Last seen: 6 years, 8 months
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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
#25145133 - 04/17/18 09:06 AM (6 years, 8 months ago) |
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Hey ya'll first post, on here! I use MEA, a 1/2 gallon mason jar, 1500ml of cold tap water, 45g light malt, 30g agar from Stamets site.(Bought a lb of that shit iv'e done well over 500 dishes and got a LOT left.) As for mixing, I use a hand blender in the mason jar, get's it nice and even in a couple seconds even with cold water. I then pour about 500ml's each into 3 long neck beer bottle's i got from a local brewery with a plastic lid that I drilled a hole in and melted a filter disc to, it has a rubber seal and metal latch, it vents out the sides of the cap where the metal latch goes through it so I don't need to throw foil on them when PC'ing. The total recipe does about 60 dishes. I used to use agar crystal shit from the health food store where I'm at but it was more expensive than Stamets even with shipping, but even with Stamets Agar I don't notice much of a quality difference I still get little black bits in my agar that are barely visible, maybe it's because of the malt i'm not sure, but it doesn't seem to affect anything as far as sterility goes so who cares.
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Baby_Hitler
Errorist




Registered: 03/06/02
Posts: 27,978
Loc: To the limit!
Last seen: 55 minutes, 2 seconds
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Re: is weak myc on agar always a nute problem? [Re: Mycolorado]
#25147086 - 04/18/18 12:23 AM (6 years, 8 months ago) |
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Quote:
Mycolorado said: Hey Ben! Telephone brand blows. It’s bulletproof at 2%....back it down to 1.25% for a stiffness you’re more accustomed to but don’t go lower. Get new agar, I just decided to give it a second chance as I was out my go to eden brand and it really inhibits normal looking growth leading to a slow moving almost clear culture. Like this:

I made xfers yesterday onto my regular agar and it’s already white and bouncing back.
Telephone brand =

WTF??
I've never heard this before. Telephone has got to be the most common brand of agar used here. I use it, and have never seen this problem.
-------------------- Morality is just aesthetics, meatbags.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
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Re: is weak myc on agar always a nute problem? [Re: Baby_Hitler]
#25147440 - 04/18/18 07:10 AM (6 years, 8 months ago) |
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IDK....maybe I got a bad lot but I’ve used a few packs with all the same results. I started playing with % as well as it’s extremely hard at 2%. The gel factor would be a bonus as you could use less...and I’ve had decent results at 1.25% as far as hardness goes but then the agar breaks very easily which makes removing the xfer wedge tedious at times. I hate paying the eden price and there’s no break online but it is fantastic. I’ll give now brand a shot as it’s half the price of eden. Just my
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Baby_Hitler
Errorist




Registered: 03/06/02
Posts: 27,978
Loc: To the limit!
Last seen: 55 minutes, 2 seconds
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Re: is weak myc on agar always a nute problem? [Re: Mycolorado]
#25147591 - 04/18/18 09:10 AM (6 years, 8 months ago) |
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I'd try some Now agar. It's like twice as much as Telephone, but if it's a lot better it might be worth it. There's no way Eden could be worth $10/oz unless it were literally all you could get.
-------------------- Morality is just aesthetics, meatbags.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
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Re: is weak myc on agar always a nute problem? [Re: Baby_Hitler] 1
#25147628 - 04/18/18 09:35 AM (6 years, 8 months ago) |
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Yeah, looks like $.35/g is about the average going rate for lab grade agar agar, though I have found some for half that. On the flip side of things, you might try the eden brand and wonder how you used the shitty ass telephone brand for so long. I don't pour a whole lot of plates so it's not really breaking the bank so to speak but it's always nice to save a couple bucks.
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MooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 11 months, 19 days
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Re: is weak myc on agar always a nute problem? [Re: Mycolorado] 1
#25147682 - 04/18/18 09:59 AM (6 years, 8 months ago) |
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Quote:
Mycolorado said: Yeah, looks like $.35/g is about the average going rate for lab grade agar agar, though I have found some for half that. On the flip side of things, you might try the eden brand and wonder how you used the shitty ass telephone brand for so long. I don't pour a whole lot of plates so it's not really breaking the bank so to speak but it's always nice to save a couple bucks. 
I found NOW brand from a local chain store that was about the same cost at telephone brand. Gonna give that a shot today. If it doesn't show many signs of improvement, I found Eden brand a nearby health food shop. May make a small batch of all 3 to try and compare growth of a clone for science.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
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Re: is weak myc on agar always a nute problem? [Re: MooseShroom] 1
#25147684 - 04/18/18 10:00 AM (6 years, 8 months ago) |
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Please do...I'd be happy to see other results/comparisons. Thanks, MooseShroom!
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 2 years, 28 days
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Re: is weak myc on agar always a nute problem? [Re: Mycolorado]
#25160718 - 04/23/18 07:18 PM (6 years, 8 months ago) |
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hey guys,
Heres an update on my agar situation. Looks like im not lacking nutes after all. My dishes are exhibiting healthy but slow growth. The reason things are going so slowly is because its only 68F or so down in the basement, a little on the cold side until it starts warming up here in Canada. I also havent grown in a while so I think im just being a little impatient.
The dishes previous to this looked real bad, the myc was so thin and transparent that it was hardly visible, but i attribute this to the first batch being on the firm side with spores you always want a softer plate.
Agar can be frustrating because of all the factors that go into it....plus the unknowns (never knew golden telephone was crappy for some) thanks for the help!
Edited by PsilocyBen17 (04/23/18 07:20 PM)
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