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OfflineDr.Greenthumbs
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Registered: 03/27/18
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Last seen: 6 years, 8 months
BRF cake metabolites (bacillus) *need input*
    #25095722 - 03/27/18 07:50 PM (6 years, 9 months ago)

Wanted some second/third opinions as to whether or not I* should lower the temps on these jars, and let the mycelium continue fighting. How many of you guys have fruited no problem with metabolites this present? The mycelium seems to be a few days stalled, but is still taking over. Jars are at about day 35. Culprit seems to be small amounts of bacillus due to too much moisture.

The issue here was me trying to colonize larger sized jars, with old spores without reducing the moisture content.( refraining from naming vendor). The first sign of mycelial growth was not apparent until day 14 at temps of 79-81. I* have found a very reputable supplier since then. To avoid  the issue, I* would have had to decrease the amount of water  in the BRF recipe, and inoculate with more ccs from the syringe in order to increase colonization rate. Like I️ said, the jars are approaching day 40. Had I* not had such low quality spores, my jars would be fully colonized. Metabolites just becoming present as of a week ago. Small amounts of bacillus seems to be present/losing to the mycelium. Now colonizing at 78 to lower bacterial thriving conditions and keep the mycelium in the warmer(aggressive colonization) temps. Would taking down to 75 significantly slow down the mycel.?











Edited by Dr.Greenthumbs (03/27/18 08:02 PM)

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OfflineWhiskey Man
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Re: BRF cake metabolites (bacillus) *need input* [Re: Dr.Greenthumbs]
    #25096052 - 03/27/18 11:11 PM (6 years, 9 months ago)

Day 40? Put those on the backburner or tossem and get something else flowing.
Or just keepem at 78 if your heart is set on them


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Amateur drinksmith.
Professional drunk.
"This hobby isn't fun anymore. Its science! No fun allowed!" -me after eating my first grow.

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InvisibleZiran
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Re: BRF cake metabolites (bacillus) *need input* [Re: Whiskey Man]
    #25096073 - 03/27/18 11:26 PM (6 years, 9 months ago)

you need to start over and keep your temps down to 65-75


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OfflineDr.Greenthumbs
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Registered: 03/27/18
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Re: BRF cake metabolites (bacillus) *need input* [Re: Whiskey Man]
    #25096108 - 03/27/18 11:45 PM (6 years, 9 months ago)

They are currently on the backburner. At this rate they'll be done at day 50. Definitely not happy with the quality from that vendor.

Worst case ill break em up outside and let them do their thing or make tea w the good myc.

You wouldn't wait a week or so to see if it overcomes the bacillus?

Anyone else have experience with their brf cakes fighting bacillus? This is honestly my first time experiencing with contams that aren't due to sterilization issues. Like I said, the bacteria showed up at day 30, as I expected it would; once I noticed germination hadn't taken place the first week.

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OfflineDr.Greenthumbs
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Re: BRF cake metabolites (bacillus) *need input* [Re: Ziran]
    #25096116 - 03/27/18 11:51 PM (6 years, 9 months ago)

This isn't my first rodeo. I have never experienced such slowly germinating spores. Under 75 degrees for germination/colonization? I have never colonized under 78 degrees for substantial growth. Keep in mind, I am working with a warm climate species.

    I will put these two jars at 75 and see if the mycelium slows down too much to combat the bacteria.

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InvisibleZiran
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Re: BRF cake metabolites (bacillus) *need input* [Re: Dr.Greenthumbs]
    #25096153 - 03/28/18 12:23 AM (6 years, 9 months ago)

Quote:

Dr.Greenthumbs said:
This isn't my first rodeo. I have never experienced such slowly germinating spores. Under 75 degrees for germination/colonization? I have never colonized under 78 degrees for substantial growth. Keep in mind, I am working with a warm climate species.

    I will put these two jars at 75 and see if the mycelium slows down too much to combat the bacteria.




Quote:

Ziran Said:
Step 5: The Wait - Incubation

This is the longest part of the whole process. The wait. Incubation should take place at 68 to 75 degree's Fahrenheit (20 to 24 Celsius). Germination usually takes about a week and full colonization can range from anywhere from 2 to 4 weeks. I store mine on a shelf in a room, pretty straight forward. Here are some cakes in various stages of the process





https://www.shroomery.org/forums/showflat.php/Number/24179086/page/3


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.


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OfflineShroomarican
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Registered: 12/14/17
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Last seen: 6 years, 9 months
Re: BRF cake metabolites (bacillus) *need input* [Re: Ziran]
    #25096194 - 03/28/18 01:06 AM (6 years, 9 months ago)

You are stressing the mycellium out by raising the temps up and down, and 78 is unessary 72-75F tops. Even if it's a warm weather species, they're domesticated and don't need high temps up above 75F and mycelium also generate heat of 3degrees plus the ambient temperature so at 75 it will be more like 78F degrees. I noticed yellow like that when I tried to use heating pads,no good. They'd probably recover if you'd leave the temps be and keep it around 70-75Max. P.Staments wrote yrs ago that ideal colonizing temps for cubes was 86F! He now agrees that's way to high.i read that book he said that in before I started cultivating,and that set me off on the wrong track "oh,86degrees? I'll need to buy heating pads and space heaters and electric f#cking blankets!" LMAO But no, room temp works best, atop a higher shelf or bookcase,perhaps. It's warmer up top than bottom, and most contams are down the bottom 2ft or so in a house, mold spores ect... well,good luck and don't sweat it. LoL (like ur jars were)jk

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OfflineDr.Greenthumbs
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Registered: 03/27/18
Posts: 86
Last seen: 6 years, 8 months
Re: BRF cake metabolites (bacillus) *need input* [Re: Shroomarican]
    #25096281 - 03/28/18 02:35 AM (6 years, 9 months ago)

Thank you guys. Very helpful. I'll post pics in a week.

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OfflineDr.Greenthumbs
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Registered: 03/27/18
Posts: 86
Last seen: 6 years, 8 months
Re: BRF cake metabolites (bacillus) *need input* [Re: Dr.Greenthumbs]
    #25158790 - 04/22/18 09:23 PM (6 years, 8 months ago)

Tossed em a while back.

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