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baddagger
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Registered: 03/04/12
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Do this look contaminated?
#25155057 - 04/21/18 10:47 AM (6 years, 8 months ago) |
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Was wondering if this looks contaminated? Noticed that have some stuff on the top of liquid and not sure if its just myc growing or bacteria? Also reason I'm concerned is that out of 4 LC this is the only one that has the specs on top, however thisis b+ where the other 3 are for stones so don't know if this LC has the specs because it's b+
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Philosophymajor199
Up and coming



Registered: 10/27/17
Posts: 198
Loc: The shit factory
Last seen: 6 years, 8 months
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Re: Do this look contaminated? [Re: baddagger]
#25155062 - 04/21/18 10:49 AM (6 years, 8 months ago) |
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Can’t really tell isolate it on agar then make your cut bud.
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Whiskey Man
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Registered: 01/06/18
Posts: 1,002
Loc: Funky Town, Electric Ave.
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What was your inoculation method and when?
I have little experience in LCs but wouldnt trust that.
-------------------- Amateur drinksmith.
Professional drunk.
"This hobby isn't fun anymore. Its science! No fun allowed!" -me after eating my first grow.
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baddagger
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Last seen: 5 months, 6 days
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Re: Do this look contaminated? [Re: Whiskey Man]
#25155097 - 04/21/18 11:06 AM (6 years, 8 months ago) |
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This was made with agar. Started with spore syringe. Did a total of three transfers where third agar stage went into liquid culture.
On reason I'm concerned is that I did draw out 3 syringes about a 7-10 days ago to test on test jars/bags they look fine, however seeing that growth start a couple days ago has me concerned
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Philosophymajor199
Up and coming



Registered: 10/27/17
Posts: 198
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Last seen: 6 years, 8 months
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Re: Do this look contaminated? [Re: baddagger]
#25155105 - 04/21/18 11:12 AM (6 years, 8 months ago) |
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Should be fine just watch it
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Whiskey Man
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Registered: 01/06/18
Posts: 1,002
Loc: Funky Town, Electric Ave.
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Well. Introducing the syringes to the culture could've contammed it. Keep tabs on it unless someone more knowledgeable says otherwise.
-------------------- Amateur drinksmith.
Professional drunk.
"This hobby isn't fun anymore. Its science! No fun allowed!" -me after eating my first grow.
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baddagger
Stranger

Registered: 03/04/12
Posts: 242
Last seen: 5 months, 6 days
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Re: Do this look contaminated? [Re: Whiskey Man]
#25155143 - 04/21/18 11:29 AM (6 years, 8 months ago) |
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The syringes I bought were all human sterile grade
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Whiskey Man
FUNctional Alcoholic



Registered: 01/06/18
Posts: 1,002
Loc: Funky Town, Electric Ave.
Last seen: 5 years, 6 months
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Re: Do this look contaminated? [Re: baddagger]
#25155166 - 04/21/18 11:38 AM (6 years, 8 months ago) |
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You stirring it every day/other day?
Could be mycelium forming a top layer for all I know.
-------------------- Amateur drinksmith.
Professional drunk.
"This hobby isn't fun anymore. Its science! No fun allowed!" -me after eating my first grow.
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baddagger
Stranger

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Last seen: 5 months, 6 days
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Re: Do this look contaminated? [Re: Whiskey Man]
#25155261 - 04/21/18 12:20 PM (6 years, 8 months ago) |
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I'll mix it up once every couple days, the LC is about a month old.
Just thinking on a side note is it possible to pull out a little health growth from one of my sealed grow bags and use that to nock up a new liquid culture?
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Whiskey Man
FUNctional Alcoholic



Registered: 01/06/18
Posts: 1,002
Loc: Funky Town, Electric Ave.
Last seen: 5 years, 6 months
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Re: Do this look contaminated? [Re: baddagger]
#25155286 - 04/21/18 12:29 PM (6 years, 8 months ago) |
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I would only do that if the bag was ready to be birthed.
-------------------- Amateur drinksmith.
Professional drunk.
"This hobby isn't fun anymore. Its science! No fun allowed!" -me after eating my first grow.
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baddagger
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Last seen: 5 months, 6 days
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Re: Do this look contaminated? [Re: Whiskey Man]
#25155343 - 04/21/18 12:54 PM (6 years, 8 months ago) |
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My idea was to just insert a clean needle threw the self healing port and just pull in a piece that's the thickness of a 18g and then pull it back into the syringe so I can then swap out the needle for a fresh sterial one and then inject it into a new fresh pressure cooked lc.
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Whiskey Man
FUNctional Alcoholic



Registered: 01/06/18
Posts: 1,002
Loc: Funky Town, Electric Ave.
Last seen: 5 years, 6 months
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Re: Do this look contaminated? [Re: baddagger]
#25155360 - 04/21/18 01:02 PM (6 years, 8 months ago) |
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In theory thatd work. Id rather do josex biopsy method myself. Just seems easier and cleaner.
-------------------- Amateur drinksmith.
Professional drunk.
"This hobby isn't fun anymore. Its science! No fun allowed!" -me after eating my first grow.
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