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Offlinesteakfry
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is my spore syringe safe?
    #25150727 - 04/19/18 01:36 PM (6 years, 8 months ago)

So I was just curious if after using the syringe a bit, then putting the cap back on after use would make it likely to contaminate. I it ok to reuse spore syringes after use? Btw doin some agar work wid it.


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Offlineverum subsequentis
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Re: is my spore syringe safe? [Re: steakfry]
    #25150749 - 04/19/18 01:46 PM (6 years, 8 months ago)

if you did your work in a sab or in front of a flow hood you can be reasonably sure that your syringe is still good IF you didn't do anything dumb while using it. like touching the needle to the wall/floor or sucking anything back into it. i like to put a little iso into the cap, let it sit for a second, flame my needle, dump out is and insert needle while still hot but not glowing hot. then i like to wrap the cap/needle base connection point with a couple wraps of cling or parafilm (whichever i'm using at the moment).

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Invisible36fuckin5
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Re: is my spore syringe safe? [Re: verum subsequentis]
    #25150781 - 04/19/18 01:58 PM (6 years, 8 months ago)

Quote:

verum subsequentis said:
if you did your work in a sab or in front of a flow hood you can be reasonably sure that your syringe is still good IF you didn't do anything dumb while using it. like touching the needle to the wall/floor or sucking anything back into it. i like to put a little iso into the cap, let it sit for a second, flame my needle, dump out is and insert needle while still hot but not glowing hot. then i like to wrap the cap/needle base connection point with a couple wraps of cling or parafilm (whichever i'm using at the moment).




That's way overkill. Buy a syringe cap (they tend to come with spore syringes) and just use that.


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bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Offlinesteakfry
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Re: is my spore syringe safe? [Re: 36fuckin5]
    #25150789 - 04/19/18 02:02 PM (6 years, 8 months ago)

ok it was exposed for a couple mins with a needle outside my SAB, but doing agar usually comes with contams and you gotta transfer a couple times right?


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  -Riff Raff

https://soundcloud.com/steakfry/

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Offlineverum subsequentis
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Re: is my spore syringe safe? [Re: steakfry]
    #25150813 - 04/19/18 02:12 PM (6 years, 8 months ago)

it takes almost no time and keeps my syringes nice and safe. sometimes i shoot for "way overkill". specially when putting them to cold storage.

spores to agar will often involve contams but not always. it is always a good idea to transfer at least once and let it grow out a bit to see if it's clean. if not, transfer again and so on.

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Offlinesteakfry
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Re: is my spore syringe safe? [Re: verum subsequentis]
    #25151124 - 04/19/18 04:15 PM (6 years, 8 months ago)

Right.... I left the needle on it in open air for like 2-3 min, how bad is that?


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  -Riff Raff

https://soundcloud.com/steakfry/

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InvisiblebodhisattaMDiscordReddit
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Re: is my spore syringe safe? [Re: steakfry]
    #25151165 - 04/19/18 04:26 PM (6 years, 8 months ago)

No, spore syringes are not very safe. They've got very sharp needles. Parental supervision required.

I put the needle cap back on. The plug type cap gets tossed after the needle goes on

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Offlinesteakfry
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Re: is my spore syringe safe? [Re: bodhisatta]
    #25151180 - 04/19/18 04:32 PM (6 years, 8 months ago)

Lol I'm wondering how safe from contams it is having been exposed for a few minutes in the open air woth the needle left on, I just want to know that ahaha.

I do admit I did poke one of my arteries and almost died once using one of those things man, scary stuff.

My buddy poked his own eye on accident and died from mushrooms growing in his brain.

Ain't no joke


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I really hate to ball on ya'll
  -Riff Raff

https://soundcloud.com/steakfry/

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OfflineMadoxx
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Re: is my spore syringe safe? [Re: steakfry]
    #25151210 - 04/19/18 04:43 PM (6 years, 8 months ago)

Pretty safe as long as you empty slightly and flame sterilize, contams are only going to get into the needle if you suck back up. Needle hole pretty small for anything much to happen around there.


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