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Offlinemushhiehunter
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Homemade spore syringes question
    #25150240 - 04/19/18 08:38 AM (6 years, 8 months ago)

Hey guys, so I made about 30 spore syringes about a year ago and I stored them in a box at room temperature. From what I can tell, the spores have germinated, does it mean that the syringes are useless? I used just sterilized tap water, no nutes...

- Why I am asking: I would think that spores can survive for a looong time, kinda like endospores, but once they germinate, they need nutes to survive - is this logic flawed or not?

And ofc I know what to do, put them on agar and see, but I'm just wondering about this one on theoretical level.

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InvisibleTangich


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Re: Homemade spore syringes question [Re: mushhiehunter]
    #25150250 - 04/19/18 08:50 AM (6 years, 8 months ago)

I had one half-syringe with a germinated clump of spores/mycelium left in the fridge for 4 years, inoculated a half pint of grains just to see if anything is still alive in there, it was fully colonized in 5 days.
So yeah, on theoretical level definitely worth a try.

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Offlinemushhiehunter
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Re: Homemade spore syringes question [Re: Tangich]
    #25150278 - 04/19/18 09:04 AM (6 years, 8 months ago)

Quote:

Tangich said:
I had one half-syringe with a germinated clump of spores/mycelium left in the fridge for 4 years, inoculated a half pint of grains just to see if anything is still alive in there, it was fully colonized in 5 days.
So yeah, on theoretical level definitely worth a try.




Thanks man! That makes me optimistic, could someone still explain this subject to me a bit? I'm eager for mycoknowledge :smile:
Quote:

Tangich said:
I had one half-syringe with a germinated clump of spores/mycelium left in the fridge for 4 years, inoculated a half pint of grains just to see if anything is still alive in there, it was fully colonized in 5 days.
So yeah, on theoretical level definitely worth a try.





That's great news! Because I found one with PE spores (btw they are from 2004) and they still germinated in the syringe... if anything grows out of it... :yolo:

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OfflineMadoxx
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Re: Homemade spore syringes question [Re: mushhiehunter] * 1
    #25151291 - 04/19/18 05:14 PM (6 years, 8 months ago)

Okay it's late in my part of the world but i'll give it my best shot at a didactic explanation, hold on tight.

In the basidiomycetes, the class of fungi to which our beloved psilocybes belong to the vegetative or 'normal' living growing etc mycelium type is dikaryotic. Which means it has two nuclei in every cell (pretty cooool). A spore contains one single nucleus or set of genetic information. When this spore germinates it creates mycelium, but monokaryotic mycelium, which only has one nucleus, in the hopes of finding another hyphae (strand of mycelium) from another germinated spore which is compatible, so they can fuse together and grow as this dikaryotic mycelium. If it takes a long time without finding another compatible spore this mycelium eventually perishes as it is not adapted to proliferate for a long time on its own. This being said a spore syringe with no nutes is no place for anything to grow happy. I hope you where asking for something like that. Also your logic is not at all flawed.

What will probably happen with your syringe is that those spores that have germinated are gone but SOME will have somehow resisted the temptation and those are your best bet at getting a viable grow, with less inoc points but almost always enough.

Distilled water in the syringe will always help to avoid germination. I hope you where asking for something like that. Ask some more theoretical doubts if you have any, actually awesome to find people wanting to know stuff just because.

:goodday:


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OfflineCHOOS
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Re: Homemade spore syringes question [Re: Madoxx]
    #25151300 - 04/19/18 05:19 PM (6 years, 8 months ago)

Quote:

Madoxx said:
Distilled water in the syringe will always help to avoid germination.
:goodday:




This.

Regular water has minerals which makes the germinate but as long as it's clean it's fine.

You can suck up some air through a flame so it's clean air and sheke the syringe if you want to break up clumps.


I've found that spores stored in water colonize way faster then a freshly made syringe. Even when not germinated.

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OfflineLogicaL ChaosM
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Re: Homemade spore syringes question [Re: mushhiehunter]
    #25151525 - 04/19/18 06:41 PM (6 years, 8 months ago)

How do spores germinate in just water?

Seems impossible.

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OfflineMadoxx
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Re: Homemade spore syringes question [Re: LogicaL Chaos] * 2
    #25152385 - 04/20/18 02:49 AM (6 years, 8 months ago)

If the water is not distilled (deionized) the spore will come into contact with these ions and minerals and possibly micro amounts of organic matter in tap water. These will be detected by receptor proteins on the surface of the spore and set off the chemical reactions which induce germination.

In the early stages, or to create the monokaryotic mycelium the spore is pretty self sufficient for a small time. The only thing the spore really needs is an aqueous medium with the minimal things in it to start, metabolizing the environment around it for energy is not so important in the first few steps.


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OfflineLogicaL ChaosM
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Re: Homemade spore syringes question [Re: Madoxx]
    #25152419 - 04/20/18 03:54 AM (6 years, 8 months ago)

Unbelievable!

:mindblown:

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