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Offlineshroomysnake
Snek

Registered: 09/23/17
Posts: 37
Last seen: 10 months, 16 days
Agar Ooze
    #25149812 - 04/19/18 12:38 AM (6 years, 8 months ago)

Hi folks,

I have tried quite a few different agar dishes now and I seem to be consistently getting some type of yellow/green ooze.  Even the dish that has grown out quite a bit seems to have that yellow ooze following the mycelium in its tracks.  Also for some reason my mycelium growth doesn't seem to be as white as other petrie dishes I've seen around here.  Can anyone tell me whats going on here?


Edited by shroomysnake (04/19/18 12:40 AM)

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OfflinehamloafM
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Re: Agar Ooze [Re: shroomysnake]
    #25149836 - 04/19/18 01:05 AM (6 years, 8 months ago)

Bad sterilie procedure.  Keep practicing, man.  Care to explain your sterile technique?


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Offlinemisterswisher
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Registered: 04/07/18
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Last seen: 1 month, 9 days
Re: Agar Ooze [Re: hamloaf] * 1
    #25150131 - 04/19/18 06:56 AM (6 years, 8 months ago)

it looks like you fucking grew ebola on a petri dish.

WTF did you do bruh?

Let me guess you did all your agar work in open air?

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Offlineshroomysnake
Snek

Registered: 09/23/17
Posts: 37
Last seen: 10 months, 16 days
Re: Agar Ooze [Re: misterswisher]
    #25150436 - 04/19/18 10:42 AM (6 years, 8 months ago)

Quote:

hamloaf said:
Bad sterilie procedure.  Keep practicing, man.  Care to explain your sterile technique?




SAB, flame sterilization, biopsy from center of stem for the first one, second one is just a pin (going off RR advise that the pin will have fast growing mycelium that will outgrow any contam). 


Quote:

misterswisher said:
it looks like you fucking grew ebola on a petri dish.

WTF did you do bruh?

Let me guess you did all your agar work in open air?




Quality response, keep it classy.

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Offlineshroomysnake
Snek

Registered: 09/23/17
Posts: 37
Last seen: 10 months, 16 days
Re: Agar Ooze [Re: shroomysnake]
    #25160245 - 04/23/18 03:46 PM (6 years, 8 months ago)

After doing another round of plates I think I have identified my issue.  Sometimes the outer tissue is quite moist and acts quite sponge like, squishing it at all will cause fluid transfer which probably carries with it a lot of bacteria.

Recent biopsy:


In the future I am going to try and take biopsies from the inner most areas that are dryish. Was doing inner tissue before, but it was not dry enough.

Edited by shroomysnake (04/23/18 03:54 PM)

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OfflineP.Avgvst
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Registered: 03/13/16
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Last seen: 2 months, 25 days
Re: Agar Ooze [Re: shroomysnake]
    #25160619 - 04/23/18 06:39 PM (6 years, 8 months ago)

Is it liquid? Or is it more waxy? Anyway, 99% sure its bacteria. You can also do a surface sterilization if the piece is big enough, and if urgent growth is not a must. And if the piece is not too “spongy” to absorb too much liquid and killing the inner cells too.

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Offlinemycoflow
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Registered: 02/26/18
Posts: 60
Last seen: 4 years, 6 months
Re: Agar Ooze [Re: shroomysnake]
    #25160945 - 04/23/18 08:46 PM (6 years, 8 months ago)

Quote:

In the future I am going to try and take biopsies from the inner most areas that are dryish. Was doing inner tissue before, but it was not dry enough.




I take a stem, rub it down with 70% ISO Alcohol, split it down the middle lengthwise and take a sample from the middle of the stem with a scalpel.  Flame sterilization, SAB, etc.  Haven't had a contam yet.

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InvisibleInocuole
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Re: Agar Ooze [Re: mycoflow] * 1
    #25161019 - 04/23/18 09:18 PM (6 years, 8 months ago)

If you're worrying about moisture you're in bacteria denial.


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InvisibleMrRabbit-HMR
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Re: Agar Ooze [Re: mycoflow]
    #25161485 - 04/24/18 02:22 AM (6 years, 8 months ago)

Quote:

mycoflow said:
Quote:

In the future I am going to try and take biopsies from the inner most areas that are dryish. Was doing inner tissue before, but it was not dry enough.




I take a stem, rub it down with 70% ISO Alcohol, split it down the middle lengthwise and take a sample from the middle of the stem with a scalpel.  Flame sterilization, SAB, etc.  Haven't had a contam yet.




Just to clarify, you should TEAR the mushroom down the middle not cut it with a scapel, if you use a scapel, you run the risk of pushing contams from the outside into the cleaner core of the mushroom.

I use iodine also for cleaning up mushrooms prior to taking samples. It's a better sanitizer and gives a better visual on whats been properly sanitized.

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Offlinemycoflow
Stranger
Registered: 02/26/18
Posts: 60
Last seen: 4 years, 6 months
Re: Agar Ooze [Re: MrRabbit-HMR]
    #25164879 - 04/25/18 04:15 PM (6 years, 8 months ago)

Quote:

MrRabbit-HMR said:
Quote:

mycoflow said:
Quote:

In the future I am going to try and take biopsies from the inner most areas that are dryish. Was doing inner tissue before, but it was not dry enough.




I take a stem, rub it down with 70% ISO Alcohol, split it down the middle lengthwise and take a sample from the middle of the stem with a scalpel.  Flame sterilization, SAB, etc.  Haven't had a contam yet.




Just to clarify, you should TEAR the mushroom down the middle not cut it with a scapel, if you use a scapel, you run the risk of pushing contams from the outside into the cleaner core of the mushroom.

I use iodine also for cleaning up mushrooms prior to taking samples. It's a better sanitizer and gives a better visual on whats been properly sanitized.




Good call!  "split" was a little vague on my part - tear, don't cut.

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