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Offlinesteakfry
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Is there mycelium in there, is it transferrable?
    #25149007 - 04/18/18 06:53 PM (6 years, 8 months ago)

Hey, made some pasty plates and I used very bad sterile technique lol, but is this plate contaminated completely, or am I able to transfer the clean parts (if there are any) to another plate?

pics:


first grow ok.....


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  -Riff Raff

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OfflineEnkidu
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Re: Is there mycelium in there, is it transferrable? [Re: steakfry]
    #25149018 - 04/18/18 06:55 PM (6 years, 8 months ago)

:shocking:


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OfflineButcherBrown
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149031 - 04/18/18 06:58 PM (6 years, 8 months ago)

I would start from scratch.


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Offlinesteakfry
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Re: Is there mycelium in there, is it transferrable? [Re: ButcherBrown]
    #25149042 - 04/18/18 07:01 PM (6 years, 8 months ago)

lol yeah i was planning on it, that infected mycelium tho?


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I really hate to ball on ya'll
  -Riff Raff

https://soundcloud.com/steakfry/

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OfflineEnkidu
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Re: Is there mycelium in there, is it transferrable? [Re: steakfry]
    #25149063 - 04/18/18 07:07 PM (6 years, 8 months ago)

Yeah bro it should look more like this



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OfflineEnkidu
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149067 - 04/18/18 07:08 PM (6 years, 8 months ago)

Did you maybe fuck up the pour? Or it looked clean and clear before you transferred ?


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OfflinePhilosophymajor199
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149071 - 04/18/18 07:09 PM (6 years, 8 months ago)

It’s fugged

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Offlinesteakfry
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149437 - 04/18/18 08:49 PM (6 years, 8 months ago)

That was inoculated with a syringe, cooked em at 10-11 psi for an hour, used a SAB and got all contams \: I used the wrong kind of tape tho ahaha, it was like silicon removal tape lmao.
Those two actually didn't go thru the PC and looked the best lol


--------------------
I really hate to ball on ya'll
  -Riff Raff

https://soundcloud.com/steakfry/

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Offlinesteakfry
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149446 - 04/18/18 08:52 PM (6 years, 8 months ago)

Any idea as to how it broke in the middle too?


--------------------
I really hate to ball on ya'll
  -Riff Raff

https://soundcloud.com/steakfry/

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OfflineEnkidu
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Re: Is there mycelium in there, is it transferrable? [Re: steakfry]
    #25149453 - 04/18/18 08:54 PM (6 years, 8 months ago)

I'm actually not familiar with his Tek.

Those contamination rates make me wonder if something is off with your process.

Every one had a contamination..?


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OfflineEnkidu
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149455 - 04/18/18 08:56 PM (6 years, 8 months ago)

I use Petri dishes and pour the agar into the petri dish in an sab myself


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Offlinesteakfry
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149544 - 04/18/18 09:20 PM (6 years, 8 months ago)

Yeah I used the easy agar tek, but used square ones instead, but that seems like its not a problem. I PC'd them for 1 hour in an electric one that only goes to like 10-11 psi, also I opened them up several times lol.


--------------------
I really hate to ball on ya'll
  -Riff Raff

https://soundcloud.com/steakfry/

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OfflineEnkidu
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Re: Is there mycelium in there, is it transferrable? [Re: steakfry]
    #25149559 - 04/18/18 09:26 PM (6 years, 8 months ago)

Lol damn man

Glad you're not taking it all too hard

Dilligence my friend, you will succeed


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Within You , Without You


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Offlinesteakfry
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149569 - 04/18/18 09:29 PM (6 years, 8 months ago)

Yeah did I just not use good technique lol


--------------------
I really hate to ball on ya'll
  -Riff Raff

https://soundcloud.com/steakfry/

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OfflineEnkidu
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Re: Is there mycelium in there, is it transferrable? [Re: steakfry]
    #25149581 - 04/18/18 09:34 PM (6 years, 8 months ago)

I have no idea.

Either you didn't sterilize the plate I guess ? Or introduced a contamination.

I'm not sure what pressure they need to reach for how long. I thought I did mine for like 60 min at 15psi but idr, been a while since I've done plates and I won't need to for a while. I would have to look it up.

Possibly checking on it didn't allow the right pressure for the correct duration, I'm sure that could be a problem.

I would recommend using a SAB if you don't use one


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Offlinesteakfry
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149599 - 04/18/18 09:41 PM (6 years, 8 months ago)

Nah like opening them as they colonized, I used a SAB and cleaned out a bathroom, idk if you are supposed to vent with am electric one, gettin the stove top one soon so I'll probably have much better success lol


--------------------
I really hate to ball on ya'll
  -Riff Raff

https://soundcloud.com/steakfry/

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OfflineEnkidu
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149610 - 04/18/18 09:46 PM (6 years, 8 months ago)

His Tek says40-45 min I didn't see what psi.

Opening while colonizing? Yeah bro that's a definite no go :lol:

Vent with electric one?

You'll probably have much better luck if you don't open it until youre in your sab ready to use it


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Offlinesteakfry
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149631 - 04/18/18 10:02 PM (6 years, 8 months ago)

yeah theres like a sealing and venting latch, i put it on the venting setting while it got up to pressure, then cooked for an hour at like 10 psi, his tek was 15 psi for 40-45 min, so i compensated pressure for time.
Don't open the agar plates till your ready to inoculate with them you mean right?
and yeah I'm just gonna tape em shut next time lol, also gonna have a 23 qt presto stove top, so I'm sure it'll work much better lol


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I really hate to ball on ya'll
  -Riff Raff

https://soundcloud.com/steakfry/

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OfflineEnkidu
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Re: Is there mycelium in there, is it transferrable? [Re: steakfry]
    #25149637 - 04/18/18 10:06 PM (6 years, 8 months ago)

Yeah exactly, don't open it til you use it, I agree, you'll have much better luck next time.

Good luck bro


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Invisibleelasticaltiger
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Re: Is there mycelium in there, is it transferrable? [Re: Enkidu]
    #25149768 - 04/18/18 11:51 PM (6 years, 8 months ago)

Please submit these images to timethyl for his "how it shouldn't look" thread.


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First time growing cakes? DON'T make a Shotgun Fruiting Chamber

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No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

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Pastywhite's Easy Agar Tek (PastyPlates)

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