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OfflineSally Hatchet
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Registered: 12/22/17
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Methods of stretching out a MS syringe?
    #25149368 - 04/18/18 08:32 PM (6 years, 8 months ago)

I'll do agar when I have a bit more space, and a bit more moolah, plus I'm still pretty inexperienced, I've grown successfully but there is clearly a lot I have left to learn.

I was thinking.. I have a MS syringe with clumps of visible spores, and I only have roughly 3ml left. I don't have any prints left, but I was wondering if I were to flame sterilize the needle (along with sterilizing/sanitizing everything else I'll be using), and open a new bottle of room temperature distilled water, could I open it inside a SAB and pull water from the bottle into the syringe basically refilling it?
Or would this be a shit catastrophe?


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OfflineShineonyoucrazy
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Re: Methods of stretching out a MS syringe? [Re: Sally Hatchet]
    #25149382 - 04/18/18 08:36 PM (6 years, 8 months ago)

Why not shoot some pf jars, get a shit ton of prints off a couple cakes, then make as many syringes or new prints as you'd like.


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Keep your feet on the ground and your head in the clouds :mushroom2:

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OfflineSally Hatchet
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Re: Methods of stretching out a MS syringe? [Re: Shineonyoucrazy]
    #25149399 - 04/18/18 08:41 PM (6 years, 8 months ago)

Quote:

Shineonyoucrazy said:
Why not shoot some pf jars, get a shit ton of prints off a couple cakes, then make as many syringes or new prints as you'd like.




I mean yeah I could do that, but I'd rather not wait that long on 3 jars. I just thought this might be a simple way to get my time and efforts worth out of it this way lol, and potentially an easier way then doing it from spore prints.

just an idea I guess.


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Invisiblescrantonstrangler
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Re: Methods of stretching out a MS syringe? [Re: Sally Hatchet]
    #25149415 - 04/18/18 08:44 PM (6 years, 8 months ago)

you will prob end up wasting the 3ml you have. thats more than enough for karo lc.

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OfflineMcGrimm
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Re: Methods of stretching out a MS syringe? [Re: scrantonstrangler]
    #25149430 - 04/18/18 08:47 PM (6 years, 8 months ago)

:whathesaid:


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In Peace or Pieces we shall move forward.

Grimm

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OfflineEnkidu
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Re: Methods of stretching out a MS syringe? [Re: Sally Hatchet]
    #25149443 - 04/18/18 08:51 PM (6 years, 8 months ago)

Agar is pretty easy. you basically need 2 ingredients, petri dish, container to pour from.

Steam sterilize ingredients in container, let cool to pour temp, pour.

Here's a 6 year old tek you can try

https://www.shroomery.org/forums/showflat.php/Number/15432426


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Within You , Without You


:mushroom2::levitate::mushroom2:

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OfflineEnkidu
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Re: Methods of stretching out a MS syringe? [Re: Enkidu]
    #25149445 - 04/18/18 08:52 PM (6 years, 8 months ago)

When I say steam sterilize I mean pressure cook.... Sorry...


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Within You , Without You


:mushroom2::levitate::mushroom2:

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OfflineShineonyoucrazy
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Re: Methods of stretching out a MS syringe? [Re: scrantonstrangler]
    #25149458 - 04/18/18 08:57 PM (6 years, 8 months ago)

Quote:

scrantonstrangler said:
you will prob end up wasting the 3ml you have. thats more than enough for karo lc.



You think syringe to lc would be worth it? I'd be worried it'd go to shit. That's the main reason I suggested pf since they are petty hard to mess up.  I haven't really messed with lc, so I can't speak from experience wether or not it'd be a viable option.


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Keep your feet on the ground and your head in the clouds :mushroom2:

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InvisibleZero Nowhere
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Re: Methods of stretching out a MS syringe? [Re: Enkidu]
    #25149464 - 04/18/18 08:58 PM (6 years, 8 months ago)

Sterilize some water and fill your syrenge back up.  Use sab, wipe everything with alcohol and flame the needle.

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OfflineSally Hatchet
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Re: Methods of stretching out a MS syringe? [Re: Enkidu]
    #25149466 - 04/18/18 08:58 PM (6 years, 8 months ago)

Quote:

Enkidu said:
When I say steam sterilize I mean pressure cook.... Sorry...




Yeah I don’t have a pressure cooker, takes up a lot of space for my shack of a home.. I’m exaggerating of course but I am super limited... I’m considering trying out a LC tek if I can find one I like.

Either way I’m still considering trying out this MS refill idea when my flush starts to come in and I nab a few more prints.


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OfflineSally Hatchet
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Re: Methods of stretching out a MS syringe? [Re: Sally Hatchet]
    #25149477 - 04/18/18 09:01 PM (6 years, 8 months ago)

Quote:

Shineonyoucrazy said:
Quote:

scrantonstrangler said:
you will prob end up wasting the 3ml you have. thats more than enough for karo lc.



You think syringe to lc would be worth it? I'd be worried it'd go to shit. That's the main reason I suggested pf since they are petty hard to mess up.  I haven't really messed with lc, so I can't speak from experience wether or not it'd be a viable option.



:whathesaid:


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Offlinemushroomnate
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Re: Methods of stretching out a MS syringe? [Re: Shineonyoucrazy]
    #25149498 - 04/18/18 09:05 PM (6 years, 8 months ago)

I regularly get 28-32 jars from a single syringe,so 3ml could get me roughly 8 jars.Plenty of jars to get spores from.I just put a tiny drop per inoculation hole,just under .5ml per jar.It may take a little longer to colonize,but it does stretch the MS syringe.I've never understood the 10-12 jars per syringe.:shrug:Seems like way too much spore solution.


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BRF to bulk/Cakes as spawn

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OfflineEnkidu
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Re: Methods of stretching out a MS syringe? [Re: Sally Hatchet]
    #25149499 - 04/18/18 09:05 PM (6 years, 8 months ago)

You could definitely try the print to syringe.

I personally never tried because I was worried it would be cotamed then I would use it to nocc up and my wait would be in vain without me realizing :/

If you want to get a lot of mushrooms I personally would be trying to save up for a pressure cooker and agar supplies. And somr quart jsrs. But I definitely understand. I did pf Tek a couple times over the course of 2 years or so getting a couple o's each time or so before move ng to bulk


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Within You , Without You


:mushroom2::levitate::mushroom2:

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OfflineEnkidu
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Re: Methods of stretching out a MS syringe? [Re: Enkidu]
    #25149502 - 04/18/18 09:06 PM (6 years, 8 months ago)

Or I guess do lc like they suggest :shrug: never done lc myself :shrug:


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Within You , Without You


:mushroom2::levitate::mushroom2:

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OfflineEnkidu
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Re: Methods of stretching out a MS syringe? [Re: mushroomnate]
    #25149511 - 04/18/18 09:08 PM (6 years, 8 months ago)

Quote:

mushroomnate said:
I regularly get 28-32 jars from a single syringe,so 3ml could get me roughly 8 jars.Plenty of jars to get spores from.I just put a tiny drop per inoculation hole,just under .5ml per jar.It may take a little longer to colonize,but it does stretch the MS syringe.I've never understood the 10-12 jars per syringe.:shrug:Seems like way too much spore solution.




I've done quite a few pf jars with one syringe as well. Literally just try to shoot the absolute smallest amount possible and I can't say I noticed longer germ time either compared to shooting more


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Within You , Without You


:mushroom2::levitate::mushroom2:

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OfflineShineonyoucrazy
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Re: Methods of stretching out a MS syringe? [Re: mushroomnate]
    #25149516 - 04/18/18 09:10 PM (6 years, 8 months ago)

Quote:

mushroomnate said:
I regularly get 28-32 jars from a single syringe,so 3ml could get me roughly 8 jars.Plenty of jars to get spores from.I just put a tiny drop per inoculation hole,just under .5ml per jar.It may take a little longer to colonize,but it does stretch the MS syringe.I've never understood the 10-12 jars per syringe.:shrug:Seems like way too much spore solution.



:whathesaid: you could defiantly get 8-10 jars with that without the worries of major contam.


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Keep your feet on the ground and your head in the clouds :mushroom2:

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OfflineSally Hatchet
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Re: Methods of stretching out a MS syringe? [Re: Enkidu]
    #25149520 - 04/18/18 09:11 PM (6 years, 8 months ago)

Quote:

Enkidu said:
You could definitely try the print to syringe.

I personally never tried because I was worried it would be cotamed then I would use it to nocc up and my wait would be in vain without me realizing :/

If you want to get a lot of mushrooms I personally would be trying to save up for a pressure cooker and agar supplies. And somr quart jsrs. But I definitely understand. I did pf Tek a couple times over the course of 2 years or so getting a couple o's each time or so before move ng to bulk




You never made a MS syringe? That’s interesting, so far I’ve only done PF, and crumbled to shoeboxes. Seems to put out more then if it were a cake alone, plus- and the best part- takes up very little space. Lol


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OfflineEnkidu
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Re: Methods of stretching out a MS syringe? [Re: Sally Hatchet]
    #25149523 - 04/18/18 09:11 PM (6 years, 8 months ago)

I've never done print to syringe is what I meant


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Within You , Without You


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OfflineEnkidu
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Re: Methods of stretching out a MS syringe? [Re: Enkidu]
    #25149526 - 04/18/18 09:13 PM (6 years, 8 months ago)

That's cool you crumble to shoebox's.

You take print to syringe's..?


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Within You , Without You


:mushroom2::levitate::mushroom2:

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Invisiblescrantonstrangler
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Re: Methods of stretching out a MS syringe? [Re: Sally Hatchet]
    #25149527 - 04/18/18 09:13 PM (6 years, 8 months ago)

well you dont need pc for lc and it seems like a lot of effort to make 1 or 2 pf jars which also may not make it. I would get 2 quart jars and 1.5 ml each.

I would get a pc and do ms to agar and g2g in sab or flow hood. but I also make lc with left over spores...


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