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OfflineTorbina
psiloenjoyer
I'm a teapot User Gallery


Registered: 12/10/14
Posts: 9
Last seen: 2 years, 10 months
Speculations on agar's resulting mycelium - Contamination?
    #25148298 - 04/18/18 02:25 PM (6 years, 8 months ago)

Hey guys, glad to post here, sadly my feelings toward my resulting petri plates isnt so positive. I need help in confirming the identification of some plates ive inoculated from Psilocybe cubensis Spore prints.
Ive divided the results into three groups:
Uncertain

Bad

Worse


Of course, i need help regarding the first pictures, but the rest can serve as an honest exposition of my poor results. Anyways thanks for taking the time to check my post.


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Edited by Torbina (04/18/18 02:31 PM)

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OfflineDr. Freeman
An agent of Order
Male


Registered: 03/27/18
Posts: 351
Loc: Midgard
Last seen: 1 year, 6 months
Re: Speculations on agar's resulting mycelium - Contamination? [Re: Torbina]
    #25148418 - 04/18/18 03:24 PM (6 years, 8 months ago)

The "Bad" and the "Worse" look beyond redemption.
Take them to the back of your house and shoot them.
Dump the corpses on the river.
Regarding the uncertain, some look pretty fine.
Still, only to be sure 100%, you could do some transfers using needle biopsy.
In case of contamination, use "The Biopsy Method: No Mercy for Bacteria."

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Invisiblexnirvanax
Frank's biological son

Registered: 05/16/13
Posts: 135
Re: Speculations on agar's resulting mycelium - Contamination? [Re: Dr. Freeman]
    #25148450 - 04/18/18 03:42 PM (6 years, 8 months ago)

:whathesaid:

I would take a couple transfers out of dishes in the 2nd and 3rd pictures.

Check out Stro's Cleaning and Isolating on Agar


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Inspired by FrankHorrigan

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OfflineDr. Freeman
An agent of Order
Male


Registered: 03/27/18
Posts: 351
Loc: Midgard
Last seen: 1 year, 6 months
Re: Speculations on agar's resulting mycelium - Contamination? [Re: xnirvanax]
    #25148514 - 04/18/18 04:06 PM (6 years, 8 months ago)

Stro's Cleaning and Isolating on Agar is awesome.
The only thing missing is needle biopsy
The images below are taken from this post.
Quote:

pacmanbreed said:






I really like the idea of using small needle instead of a comparatively huge scalpel. Makes a lot of difference in the microscopic world of the agar plate.

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OfflineTorbina
psiloenjoyer
I'm a teapot User Gallery


Registered: 12/10/14
Posts: 9
Last seen: 2 years, 10 months
Re: Speculations on agar's resulting mycelium - Contamination? [Re: Dr. Freeman]
    #25148781 - 04/18/18 05:43 PM (6 years, 8 months ago)

Wow thats some incredible method, thank you so much for the direction. Ill be updating with results using the brf pucks.

Quote:

xnirvanax said:
:whathesaid:

I would take a couple transfers out of dishes in the 2nd and 3rd pictures.

Check out Stro's Cleaning and Isolating on Agar



also thanks for the recommendation. I still dont understand whats going on with the big fluffy ones, they grew way faster than the others and seem dense as fuck. Im aware of Stro, his videos and tek's are great.


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