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Invisiblemindblowing
Stranger Danger

Registered: 12/26/15
Posts: 238
Did i stuff up my agar no pour plates? (condensation)
    #25146416 - 04/17/18 07:07 PM (6 years, 8 months ago)

I followed "Pastywhyte's Easy Agar Tek"

Here is my every step, I only made 250ML enough for 10 plates, 5g agar agar, 5g malt extract liquid i stirred it up pour into the plates and PC'ed. I was reading about problems with boiling over i thought that was on the come-up going from pastywhyte's tek, But i read more around the forums while PC'ing found out it's about when it's cooling down apprently.

Pastywhyte's Easy Agar Tek, Pretty much says you have to watch on the come-up not the come-down process of pc'ing. I'm not sure what info is correct sounds like after more research it's the coming down process depressurizing down to fast.

Anyway i found these little tubs at my local shops, I drilled a 1/4 hole i put a piece of folded paper towl (is this where i had the problem? it was cheap shit papertowl i was nervous about it to begin with just how thin it is) So anyway i folded up a piece of papertowl and wrapped the containers with foil..

As i was just following the tek i brang my pc up extremely slowly on low-medium heat probably took a few hours, I got it to 15PSI later on, and PC for probably 30-40 @ 15PSI, Then i turned the PC of and left it on the hot plate overnight and naturally just let it cool down for about 10hours then i put my agar plates in a pretty dark/room temp spot in a draw, Now i just went to inoculate these agar plates i was taking them to my SAB and had a quick peak at the bottom i could see tons of consideration, So i put them in my SAB took of the tinfoil and the paper towl in the SAB and all the papertowl was stuck to the micropore tape i put on there (2 layers) it's pretty damp quiet a lot of consideration got into my agar plates... I was just researching and it says consideration is pretty bad stunts the growth of mycelium, So i carefully one by one upwrapped my containers and took the lid of and tipped out the water, There was not a TON but there is defiantly more consideration then there should be!

Now i thought i'd write what i did incase i'm doing something wrong..
My guess is as i didn't follow the tek exactly my containers are different shape, My paper towl was absolute thin and shit is this why i have got excess moisture? or is it something else? Is it honestly worth even trying these plates, Letting them dry out for a while in the SAB? I have them sitting in my SAB unwrapped with soggyish micropore taping covering the holes still, I have put gladwrap over the arm holes hopefully to stop contaminations, as micropore tape won't stop containments will it? is there any chance these plates could dry out?

Every thing I've pc'ed has got a bit of moisture in it, My grains i did where pretty good but there was like 10 or so grains on top absolutely drenched, I have not taken the tinfoil off but i could imagine my polyfill is drenched and it dripped on a few grains that's why. Am i doing something wrong?

I don't really like the idea of non pour, I think i'd rather do hot agar and poor it but if these plates have a chance of drying up a bit i'll give them a go, I need a bit of guidance here guys :frown:


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Invisiblemindblowing
Stranger Danger

Registered: 12/26/15
Posts: 238
Re: Did i stuff up my agar no pour plates? (condensation) [Re: mindblowing]
    #25146444 - 04/17/18 07:15 PM (6 years, 8 months ago)



Here is a picture, im trying to hard to research ive been researching on and off for 1-2 years just only had time to do it now, this hobby is all means not easy, i just got to figure out what im doing wrong and improve.


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OfflineDr. Freeman
An agent of Order
Male


Registered: 03/27/18
Posts: 351
Loc: Midgard
Last seen: 1 year, 6 months
Re: Did i stuff up my agar no pour plates? (condensation) [Re: mindblowing]
    #25146447 - 04/17/18 07:17 PM (6 years, 8 months ago)


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Invisiblemindblowing
Stranger Danger

Registered: 12/26/15
Posts: 238
Re: Did i stuff up my agar no pour plates? (condensation) [Re: Dr. Freeman]
    #25146456 - 04/17/18 07:21 PM (6 years, 8 months ago)

Quote:

Dr. Freeman said:
This might help a bit




Why didn't i see that 3 days ago

that looks very informational thankyou.


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OfflineSome Guys Fungi
Your Highness
Male


Registered: 04/17/18
Posts: 7
Last seen: 6 years, 8 months
Re: Did i stuff up my agar no pour plates? (condensation) [Re: mindblowing]
    #25146579 - 04/17/18 08:15 PM (6 years, 8 months ago)

Hey man, good on you starting agar, iv'e never done pasty's tek so I can't say much about the tek itself or how much condensation is "normal" in a pasty plate, but i'm sure standing water on the plates is not normal. Plates do sort of dry up a little on there own from sitting but not much at all. They're definitely a ripe breeding ground for bacteria when they have a bit of standing water on the surface of the agar. The PC sitting all night on the hotplate is totally fine for your cool down it doesn't need to cool down any slower, as long as there's enough thermal mass (lots of water in PC) depressurizing too fast won't be an issue doing it like that. It's hard to say exactly what the issue is, only 2 things I could think of.

1. Were the containers sitting on anything in the PC to keep them elevated?

2. Is there any agar on the inside of the lids in your dishes?


side note: properly hydrated and dried grain jars will always be a little moist, as long as there isn't any pooling water in there you should be aight!

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Invisiblemindblowing
Stranger Danger

Registered: 12/26/15
Posts: 238
Re: Did i stuff up my agar no pour plates? (condensation) [Re: Some Guys Fungi]
    #25146663 - 04/17/18 08:42 PM (6 years, 8 months ago)

Quote:

Some Guys Fungi said:
Hey man, good on you starting agar, iv'e never done pasty's tek so I can't say much about the tek itself or how much condensation is "normal" in a pasty plate, but i'm sure standing water on the plates is not normal. Plates do sort of dry up a little on there own from sitting but not much at all. They're definitely a ripe breeding ground for bacteria when they have a bit of standing water on the surface of the agar. The PC sitting all night on the hotplate is totally fine for your cool down it doesn't need to cool down any slower, as long as there's enough thermal mass (lots of water in PC) depressurizing too fast won't be an issue doing it like that. It's hard to say exactly what the issue is, only 2 things I could think of.

1. Were the containers sitting on anything in the PC to keep them elevated?

2. Is there any agar on the inside of the lids in your dishes?


side note: properly hydrated and dried grain jars will always be a little moist, as long as there isn't any pooling water in there you should be aight!




Thanks for you're reply! Well TBH i know that's another mistake, I use less water then normal 2.6quart just a little less then the normal 3~ish

Then like you said i read you actually want a lot more water then usual "thermal mass" So i did stuff up there aswell

Yes the containers where fully evaluated away from the water, I used a few canning jars then i used my flat canning rack came with my presto and stacked all my agar plates above that.

I read what Dr.Freeman linked before https://www.shroomery.org/forums/showflat.php?Cat=0&Number=25137693&page=0&vc=1 that was an awesome write up, You sure it wouldn't of been from leaving it overnight? His tek says you don't have to leave it overnight, While i was pc'ing a family member of mine was questioning why i was leaving the agar plates in there all night as it's natural when it cools downs overnight the plates are going to absorb the moisture etc.

I appercaite you're reply, Just looked when i registered it's been 3 years I've been hovering this forum & soaking in information, turns out when you do it physically theres always something to learn, I'm pretty serious about mushroom cultivation it excites me, I want to take this pretty large scale but overtime, I think i'll move onto pouring agar in plates next, But at the same time i want to figure what happened, You sure it's not from leaving the plates in overnight? It makes sense to me overnight they soak in the moisture. Maybe in you're have a large thermal mass it might not soak in the moisture overnight?

Hmmm, thank you


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Edited by mindblowing (04/17/18 08:45 PM)

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OfflineDr. Freeman
An agent of Order
Male


Registered: 03/27/18
Posts: 351
Loc: Midgard
Last seen: 1 year, 6 months
Re: Did i stuff up my agar no pour plates? (condensation) [Re: mindblowing]
    #25146739 - 04/17/18 09:11 PM (6 years, 8 months ago)

This is a place for a lot of people to express their opinions and one must learn who to listen to.
When deciding whose advice to follow check:
  • Number of post. The guy who made the Tek has over 4000
  • Years on this forum.
  • The number of General Ratings I only got 6x(5/5) Josex has over 60.
  • The "Trusted Cultivator" tag is also a big deal, always listen to what they have to say.


Don't leave your agar on the PC overnight.

No disrespect intended to anyone, I only started growing two yeas ago and still have much to learn,.

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Invisiblemindblowing
Stranger Danger

Registered: 12/26/15
Posts: 238
Re: Did i stuff up my agar no pour plates? (condensation) [Re: Dr. Freeman]
    #25146789 - 04/17/18 09:30 PM (6 years, 8 months ago)

Quote:

Dr. Freeman said:
This is a place for a lot of people to express their opinions and one must learn who to listen to.
When deciding whose advice to follow check:
  • Number of post. The guy who made the Tek has over 4000
  • Years on this forum.
  • The number of General Ratings I only got 6x(5/5) Josex has over 60.
  • The "Trusted Cultivator" tag is also a big deal, always listen to what they have to say.


Don't leave your agar on the PC overnight.

No disrespect intended to anyone, I only started growing two yeas ago and still have much to learn,.




extremely well said, I appercaite you're help.

Yeah it's the reason my agar stuffed over, i left it overnight. Dr.Freeman if you have enough experince would you say i should even bother trying to inncoulate these?


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OfflineDr. Freeman
An agent of Order
Male


Registered: 03/27/18
Posts: 351
Loc: Midgard
Last seen: 1 year, 6 months
Re: Did i stuff up my agar no pour plates? (condensation) [Re: mindblowing]
    #25147311 - 04/18/18 04:57 AM (6 years, 8 months ago)

They should be good to go.
Give em a try.

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OfflineSome Guys Fungi
Your Highness
Male


Registered: 04/17/18
Posts: 7
Last seen: 6 years, 8 months
Re: Did i stuff up my agar no pour plates? (condensation) [Re: Dr. Freeman]
    #25147701 - 04/18/18 10:07 AM (6 years, 8 months ago)

Quote:

Dr. Freeman said:
This is a place for a lot of people to express their opinions and one must learn who to listen to.
When deciding whose advice to follow check:
  • Number of post. The guy who made the Tek has over 4000
  • Years on this forum.
  • The number of General Ratings I only got 6x(5/5) Josex has over 60.
  • The "Trusted Cultivator" tag is also a big deal, always listen to what they have to say.


Don't leave your agar on the PC overnight.

No disrespect intended to anyone, I only started growing two yeas ago and still have much to learn,.




After reading Josex's guide I'd say don't leave them in there overnight, although I definitely have and not had insane condensation on the lids.
I'd agree in general the people who have posted more and been on the site longer are definitely people you should be listening too, especially trusted cultivators, although their word isn't gospel and they are wrong sometimes. Like I said, Iv'e never done pasty's tek, Iv'e done no pour agar's but not his tek specifically. I understand you're just looking out for this guy and Josex is definitely a voice of reason on this site, but don't disregard everything someone says based on the fact that they don't have many posts or haven't had an account on here for long. Having enough thermal mass while PCing your agar IS important, Iv'e had a mess in my PC a few times because of this specifically, I'm running an AA41 and the smaller the PC the faster the cool down. I'm a noobie to posting on this site but Iv'e been doing this for 6+ years at this point and I'm fairly well versed when it comes to basic mycological practices. Same though, no disrespect and I appreciate you linking the Josex guide, definitely learned something about no pours. When I started agar Pasty's tek wasn't around and neither was Josex. I only do hot pours with a flow hood and have for a while now so I'm not up on the new no pour methods.

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OfflineDr. Freeman
An agent of Order
Male


Registered: 03/27/18
Posts: 351
Loc: Midgard
Last seen: 1 year, 6 months
Re: Did i stuff up my agar no pour plates? (condensation) [Re: Some Guys Fungi]
    #25147805 - 04/18/18 10:58 AM (6 years, 8 months ago)

Quote:

Some Guys Fungi said:
don't disregard everything someone says based on the fact that they don't have many posts or haven't had an account on here for long.



I agree, those are just some general parameters to help out new guys on their search for knowledge in a vast sea of opinions.
But those are the only parameters we can use to guess without actually knowing the person.
You could be a professor in mycology at Harvard University but since we can't tell that, we go for what little we do know.
And yes, no one's word should be taken as if Moses with the 10 commandments.
You've been growing a few years longer than I so you know a lot of this is trial and error over science.
I simply suggested following the directions of someone I know for making a lot of experimenting vs someone I don't know.
No disrespect intended toward your person, I simply made a safe bet with someone I trust.

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OfflineSome Guys Fungi
Your Highness
Male


Registered: 04/17/18
Posts: 7
Last seen: 6 years, 8 months
Re: Did i stuff up my agar no pour plates? (condensation) [Re: Dr. Freeman]
    #25147828 - 04/18/18 11:10 AM (6 years, 8 months ago)

No worries my man, no disrespect taken just giving my 2 cents. I totally understand where you're coming from. Trial and error is a bitch but it's the only way to gain true experience! I always say you can read and ask all you want but until you get out there and do it it's all bs. lol.

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