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OfflineGrizzle
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Faint Mycelia On Agar
    #25145882 - 04/17/18 05:24 PM (3 years, 20 days ago)

So ever sine discovering a wild Ps Allenii patch in my yard, I've been climbing the learning curve of cultivation in the interest of promoting these powerful specimens. Up until I discovered these, I had only taken cubes of unknown origins, and the Allenii experience is awesome!

In addition to scattering myceliated wood chips around the yard, I've been attempting both cardboard/egg carton growth from myc chips and agar with little to show for it thus far. I'm still learning and having a good time.

I'm hoping to get some advice on my agar work. I'm working from a funky spore print and also tiny bits of mycelia.

Question 1:  All the myc drops onto agar immediately went bacterial, but this one below surprised me with what looks to be mycelia growing out from under the bacteria when backlit. It's totally flat on the agar, but is very faint, thin, and greyish. Is this mold, mushroom, or something else?



Question 2:  My spores on agar have gone nowhere and it's been 3-4 weeks. Could Allenii really be this slow? Is there anything I could be doing wrong other than possibly overcooking the agar in the PC?

Agar is half premixed MEA and half agar agar. I diluted the mix due to some very thick, non-rhizomorphic growth in a previous attempt that turned out to probably be mold. I pressure cooked for about 40 minutes due to accidentally going below 15psi for a sec.

Your thoughts are welcome and appreciated!


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OfflineMrSturgill
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Re: Faint Mycelia On Agar [Re: Grizzle]
    #25145977 - 04/17/18 06:05 PM (3 years, 20 days ago)

:smoker:


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InvisibleetuHad4
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Re: Faint Mycelia On Agar [Re: MrSturgill]
    #25146019 - 04/17/18 06:29 PM (3 years, 20 days ago)

from the top view it looks bacterial in the center.
from the bottom view it looks bacterial in the center and "POSSIBLE" Mycelia in a circular  formation covering 4/5ths of the plate. IMO. I would try a transfer. Or give us a Better close in top view.


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OfflineMrSturgill
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Re: Faint Mycelia On Agar [Re: etuHad4]
    #25146057 - 04/17/18 06:51 PM (3 years, 20 days ago)

yeah man, definitely looks like some kind of bacteria in the center, transfer as soon as possible, transfer from 11, 9 , and 7 at least and keep us updated. Looks too thin to be mycelium but I'm really not familiar with this variety so I'm not sure what the mycelium would look like on agar, I know cubensis would be unmistakable. It's always kinda hard to tell from pics, just use your best judgement and keep going, I would love to see you succeed in cultivating these.


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OfflineGrizzle
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Re: Faint Mycelia On Agar [Re: MrSturgill]
    #25146094 - 04/17/18 07:12 PM (3 years, 20 days ago)

Yeah the blob is definitely bacteria. That was the only thing visible for a few days and then the possible mycelia started showing up around the sides of the blob.

I'll transfer and hope for the best, thanks for the location tips!

Any thoughts on the stagnant spore drop?


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OfflineAma420
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Re: Faint Mycelia On Agar [Re: Grizzle]
    #25146155 - 04/17/18 07:40 PM (3 years, 20 days ago)

Recipe tweeking may be at fault. Use recipes that are proven to work, and dont overcook


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Re: Faint Mycelia On Agar [Re: Ama420]
    #25146881 - 04/18/18 12:10 AM (3 years, 20 days ago)

Fair enough! I'll track down an ideal Allenii/woodlover agar recipe and make it myself. Hoping for some nice white strandy results.


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Invisiblehamloaf
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Re: Faint Mycelia On Agar [Re: Grizzle]
    #25146890 - 04/18/18 12:15 AM (3 years, 20 days ago)

Standard malt extract agar recipe works great for woodloving, psychoactive species of mushrooms.


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