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InvisibleMethodicalMystical
Just a normal living being
I'm a teapot User Gallery


Registered: 07/16/17
Posts: 247
Loc: PNW
Re: Need Help? ASK THE TRIBE! [Re: TheMadHatter420]
    #25158776 - 04/22/18 09:10 PM (5 years, 10 months ago)

Just put a media bottle through the PC for my first agar plates (BRF agar from bod's tek; 15 PSI for 25 minutes). According to site research I should wait until the pressure drops to 0 before opening up, but I'm wondering about the temperature of the agar once it gets to that point. Would I be able to pour it then? No microwave available to heat it up, so I'm mainly wondering if it will remain hot long enough to pour into ~20 plates.


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I know I'm a fool, but that's why I keep trying to learn.
:peace::heart::mushroom2:

I also have some 3-year Cambodian prints to get rid of. DM me if interested

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Offlineverum subsequentis
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Registered: 03/22/16
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Re: Need Help? ASK THE TRIBE! [Re: MethodicalMystical]
    #25158814 - 04/22/18 09:37 PM (5 years, 10 months ago)

It'll still be way too hot. No worries. If you don't have a way to monitor the temp just put when it doesn't burn your hand to hold. It'll still feel quite warm but not painful.  140 ish degrees.

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InvisibleMethodicalMystical
Just a normal living being
I'm a teapot User Gallery


Registered: 07/16/17
Posts: 247
Loc: PNW
Re: Need Help? ASK THE TRIBE! [Re: verum subsequentis]
    #25158830 - 04/22/18 09:49 PM (5 years, 10 months ago)

I have some thermometers laying around, so I was thinking of using that and a hot water bath/the PC water to check on things. Would overnight (~18 hours off the burner) be an okay amount of time?


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I know I'm a fool, but that's why I keep trying to learn.
:peace::heart::mushroom2:

I also have some 3-year Cambodian prints to get rid of. DM me if interested

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Offlineverum subsequentis
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Re: Need Help? ASK THE TRIBE! [Re: MethodicalMystical]
    #25158850 - 04/22/18 10:00 PM (5 years, 10 months ago)

No. It'll harden after a couple hours. Depends on the PC. My all American is a tank and keeps the agar hot enough for a couple hours but the average PC will lose heat quicker. Monitoring the PC water temp is a great idea. Take it out in time to pour at around 140. If I only have a few plates to pour I start around 120

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Offlinesangreen
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Registered: 03/31/18
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Re: Need Help? ASK THE TRIBE! [Re: verum subsequentis]
    #25158919 - 04/22/18 10:50 PM (5 years, 10 months ago)

I spawned 2 PF cakes to coir. There's kind of a funky smell, and I think it might just be coir+mushies, but the other coir that I prepped at the same time smells different than the coir I spawned to. I guess the only way to know is to watch and smell, right?

Anyways, what are telltale early signs of bulk contam? I'm 99% sure the cakes weren't contaminated.


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I figured it out, what life is about
It crawled into my brain and it's starting to sprout

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InvisibleYesum
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Re: Need Help? ASK THE TRIBE! [Re: sangreen]
    #25158959 - 04/22/18 11:35 PM (5 years, 10 months ago)

Coir usually wont go green on its own. Usually. If you heat treated it well enough it wont.  And if yoy heat treated it well enough and used clean spawn/cakes. Your good.


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InvisibleBigSurMoon
the deathless ones
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Registered: 03/21/18
Posts: 876
Loc: Chicken coop
Re: Need Help? ASK THE TRIBE! [Re: Yesum]
    #25159164 - 04/23/18 02:43 AM (5 years, 10 months ago)

Is it that honey lc doesnt play nice with brf cakes? I knocked up like thirty jars two weeks, and one week ago as well. I did at least six agar plates and they all turned out fine. But the jars nothing. Anyone have experience with this. Either way im gonna go to a different lc,li. Probably try use the honey lc on some experimenting.
Thanks much


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My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.

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InvisibleYesum
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Re: Need Help? ASK THE TRIBE! [Re: BigSurMoon]
    #25159170 - 04/23/18 02:48 AM (5 years, 10 months ago)

Yes. I have the same kind of luck with LCs. Why i dont use them. Liquids are cool. But not necessary


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Edited by Yesum (04/23/18 06:51 AM)

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InvisibleBigSurMoon
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Re: Need Help? ASK THE TRIBE! [Re: Yesum]
    #25159179 - 04/23/18 02:59 AM (5 years, 10 months ago)

Yeah i made them right away thinking they would establish future inocs. I have graduated since to spore printing. Would the hlc work on maybe grains or seeds, in an outdoor grow? Im itching to try some outdoor adventures.
Thanks, tribe rules!

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InvisibleYesum
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Re: Need Help? ASK THE TRIBE! [Re: BigSurMoon]
    #25159188 - 04/23/18 03:10 AM (5 years, 10 months ago)

I make LI or just drop wedges. Then g2g. No clue with the outdoor stuff. Someone else might have a answer for ya


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InvisibleBigSurMoon
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Registered: 03/21/18
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Re: Need Help? ASK THE TRIBE! [Re: Yesum]
    #25159194 - 04/23/18 03:19 AM (5 years, 10 months ago)

Yeah im still in noob phase... i like the cakes. I thought the hlc would be great for cakes,oh well. Im the kind of person who has to try it all at least once.
Thanks much, im impressed how fast you guys answer. Thats really cool,


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.

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InvisibleYesum
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Registered: 11/05/12
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Re: Need Help? ASK THE TRIBE! [Re: BigSurMoon]
    #25159195 - 04/23/18 03:27 AM (5 years, 10 months ago)

Wait. Did u mean a GLC.  IF i know my grain jar is clean. I.E. made from a clean plate. I have then used that grain jar to make a blended GLC of sorts. Some call them slurries. All the same really. And yeah that does work. Its probably a little more advanced technique but its a neat way to stretch a donor jar.


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InvisibleBigSurMoon
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Re: Need Help? ASK THE TRIBE! [Re: Yesum]
    #25159197 - 04/23/18 03:35 AM (5 years, 10 months ago)

No, i was reading some of violets teks with grass seed. And wondering if the hlc would be a good inoc. Im not really interested in doing bulk, if i can use up this hlc on something productive. I have three pints of the shit. Im gonna probably just dump it on some substrate outdoors and see what happens.
Thanks much

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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: Need Help? ASK THE TRIBE! [Re: BigSurMoon]
    #25159332 - 04/23/18 06:38 AM (5 years, 10 months ago)

Quote:

BigSurMoon said:
Is it that honey lc doesnt play nice with brf cakes? I knocked up like thirty jars two weeks, and one week ago as well. I did at least six agar plates and they all turned out fine. But the jars nothing. Anyone have experience with this. Either way im gonna go to a different lc,li. Probably try use the honey lc on some experimenting.
Thanks much





LC is an advanced tek that is easily fucked up. Working with LC as a side project, so you can learn, is fine but don't rely on it when your new, it will just burn you as LC contaminates EASILY!!! How did you inoculate said LC????


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InvisibleTheMadHatter420
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Registered: 10/12/16
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Re: Need Help? ASK THE TRIBE! [Re: BigSurMoon]
    #25159337 - 04/23/18 06:42 AM (5 years, 10 months ago)

Quote:

BigSurMoon said:
No, i was reading some of violets teks with grass seed. And wondering if the hlc would be a good inoc. Im not really interested in doing bulk, if i can use up this hlc on something productive. I have three pints of the shit. Im gonna probably just dump it on some substrate outdoors and see what happens.
Thanks much





That most likely wont work, as it is VERY unsuccessful for wood lovers. It is better to transfer something colonized.

The down side to LC is that a single bacteria cell will reproduce fast as fuck and take over the LC. They contaminate SUPER easy and if you started from spore, well they are 99% fucked and better off dumping them. I been back at this for 1.5 years and LC still is tough for me to get clean all the time.


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OfflineCrispykoot
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Re: Need Help? ASK THE TRIBE! [Re: TheMadHatter420]
    #25159342 - 04/23/18 06:45 AM (5 years, 10 months ago)

Helpful tips with LC if you have the sterile work down is to use eats SFD tek and use parafilm for very little to no contams.


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:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:

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InvisibleTheMadHatter420
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Registered: 10/12/16
Posts: 12,941
Re: Need Help? ASK THE TRIBE! [Re: Crispykoot]
    #25159347 - 04/23/18 06:48 AM (5 years, 10 months ago)

I started covering my jars with a clean, NEW, shower cap. Sandwich bags could be used also. This helps keep dust and things away from the lids. I like to pour mine, so I like my lids clean.


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InvisibleBigSurMoon
the deathless ones
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Registered: 03/21/18
Posts: 876
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Re: Need Help? ASK THE TRIBE! [Re: TheMadHatter420]
    #25159469 - 04/23/18 08:37 AM (5 years, 10 months ago)

Yeah with ms syringe, at first i was ginna rely on it, but ive now successfully made prints, and ms syringe. Wich have done well si far(check my journal if interested). The lc has grown well on agar with no contams. As of yet, and the transfers from those look good as well. So i think my lc is ok(i can send pics later) but like i was saying its been a nogo in pf tek. I just dont want to waste them. Im curious if theres a specific reason it wont take in brf, but does well on agar?
Thanks much


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.

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InvisibleBigSurMoon
the deathless ones
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Registered: 03/21/18
Posts: 876
Loc: Chicken coop
Re: Need Help? ASK THE TRIBE! [Re: BigSurMoon]
    #25159477 - 04/23/18 08:44 AM (5 years, 10 months ago)

It probably is contaminated, but that baffles me why all the agar plates show no sign of contams, i littered the plates with drops too, when i get home ill post pics of lc, and plates and transfers.
Thanks for helping

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InvisibleTheMadHatter420
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Registered: 10/12/16
Posts: 12,941
Re: Need Help? ASK THE TRIBE! [Re: BigSurMoon]
    #25159520 - 04/23/18 09:24 AM (5 years, 10 months ago)

No contams that you can see, BUT not all bacteria is easily visible on agar, some hide in among the mycelium. There is no way the LC is 100% clean. NOW that being said, I am NOT saying it doesn't have a use. Just use it on something that will be an unbroken colony, like cakes. IF you are NOT going to break apart the mycelium colony you can get away with a bit of contamination. IF you shake the jar or break up the colony, you spread the contamination at the same time the mycelium is weakened from being broke apart. This is why cakes are more contam resistant than say grains.


Quote:

BigSurMoon said:
It probably is contaminated, but that baffles me why all the agar plates show no sign of contams, i littered the plates with drops too, when i get home ill post pics of lc, and plates and transfers.
Thanks for helping





That right there is why. You are supposed to put only a drop or 2. Putting a bunch causes a jumbled growth mess that will hide the bacteria. I do not intend to offend, but I think you need to work on the agar skills some 1st. LI would be a better option than LC, as it doesn't give contams a growth window like LC.


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