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OfflineGott
Stranger
Registered: 02/26/18
Posts: 17
Last seen: 4 years, 11 months
Best method to store spores/culture for about 2 years
    #25145318 - 04/17/18 10:21 AM (6 years, 8 months ago)

Hey guys,

I'm kind of an intermittent mushroom-user, so I can get by quite a while with the results of my grows. Since I regularly have a lot of trouble with non-viable spore prints after about 1,5 to 2 years of storage I just wanted to ask what's the BEST way to keep 'something to grow mushrooms from' alive over a period of about 2 years without having viability problems and that can be used in the pf-tek? 

Thanks in advance!


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"There is no god, no meaning and no free will. Enjoy the rest of your miserable existence, frivolous automatons." - N.A.

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OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 8 days, 8 hours
Re: Best method to store spores/culture for about 2 years [Re: Gott]
    #25145329 - 04/17/18 10:31 AM (6 years, 8 months ago)

Slants are an option. Agar in a testube/jar inoculated with mycelium. RR used to put a little bit of wood in it to elongate the life further.

As for spores, syringes have a longer life than prints. Your unviable prints could be revitalized by making them into a sryinge.


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

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Offlinewushroow
Male

Registered: 02/03/17
Posts: 369
Last seen: 6 years, 7 months
Re: Best method to store spores/culture for about 2 years [Re: The Mycologist]
    #25145618 - 04/17/18 01:02 PM (6 years, 8 months ago)



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:tripmolecule:  :thataintright:  :tripmolecule:

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OfflineNeutrino
Maple tree
I'm a teapot User Gallery
Registered: 04/14/18
Posts: 53
Last seen: 6 years, 8 months
Re: Best method to store spores/culture for about 2 years [Re: wushroow]
    #25145631 - 04/17/18 01:07 PM (6 years, 8 months ago)

Make a print inside a sterilized mason jar. Make sure all moisture is gone from the jar and seal. Store at room temp in the dark. People have seen things come to life after 5+ years. Spores can last a very very long time and potentially survive quite harsh conditions.


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Tandem bicycles are designed for two riders. Do it together.

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OfflineMadoxx
Aspiring Mycologist


Registered: 05/19/13
Posts: 111
Last seen: 3 years, 8 months
Re: Best method to store spores/culture for about 2 years [Re: wushroow]
    #25145639 - 04/17/18 01:10 PM (6 years, 8 months ago)

:whathesaid: Especially when you introduce that piece of wood into the slant, if not you drastically reduce the viability of it to about 6 months.


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