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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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Bubbles in agar
#25144309 - 04/16/18 08:29 PM (6 years, 8 months ago) |
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Got some bubbles in some places I poured the majority of them.
It's not contamination it's just air bubbles that didn't pop or whatever for whatever reason.
I tried pouring slow and fast same thing happened.
Any way to avoid this? Makes it kinda a bitch to see 
BTW this isn't one or 2 bubbles were talking an 8th of my dish is bubbles.
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (04/16/18 08:30 PM)
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
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I find if I swirl too hard I get bubbles but they usually dissipate if left to rest for a sec or two. Pouring cooler also helps. You could also pop them on the plate with the lip of the media bottle but I’d try to avoid having to do that.
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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I do swirl pretty hard the bottom tends to solidify before I can finish pouring them all. I try to go for the lowest temp.

I couldn't and wouldn't pop those with the bottle lol.
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
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PsilyCameraGuy
Stranger

Registered: 01/10/18
Posts: 95
Last seen: 4 years, 10 months
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Same thing happened to my last pour, I just recently switched to MEA and I assumed I’d poured it to hot/fast (I was kind of in a rush).
So far it doesn’t seem to be affecting the transfers negatively, but I’m keeping a close eye on them.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
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Hmm...seems excessive. Maybe pour a tad warmer or quicker if possible so as to not have to agitate so hard at the end due to it setting.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 2 years, 7 months
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pour a little hotter and don't swirl it so hard. if you do, wait for a minute before pouring.
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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thanks guys
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,243
Loc: Right Here
Last seen: 3 hours, 12 minutes
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I've found an IR thermometer really helps alot. Not sure how accurate it is, but you can get the feel after a pour or two.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 2 years, 7 months
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Re: Bubbles in agar [Re: Asura]
#25149675 - 04/18/18 10:32 PM (6 years, 8 months ago) |
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i bought one and used it for a while. i still use it when i can find it but really, you don't have to be too precise. using it and testing shit out i found that anywhere between 150 and 110 will work just fine. even lower really but it will start to gel up.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,243
Loc: Right Here
Last seen: 3 hours, 12 minutes
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I agree. I've poured everywhere between 120 and 140 and as far as condensation goes I haven't noticed much of a difference. Condensation happens.
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