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OfflinePsilocyBen17
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is weak myc on agar always a nute problem?
    #25144223 - 04/16/18 09:51 PM (3 years, 26 days ago)

Hey All,

Getting back into the hobby after almost a year and so far I'm disappointed in how its going. a few weeks ago I transfered some spores to agar, only couple plates germinated. I made transfers from those, but my myc growth looks weak and very tomentose. Part of the problem is I forgot to colour my agar, so its impossible to see (duh) but aside from that my plan is to up the nute content for the next batch of plates, probably using grain soak or potato water.

My question is this: will low nute content always cause whispy growth? Could the agar agar powder itself be causing a problem? I bought it on ebay, the package is entirely korean. It seems firmer then usual....but could there be a sugar or something messing up the myc? Maybe I should toss it and go with good ol Golden Telephone?

Thx guys, appreciate it. got the heebiejeebies now (fuck do they ever suck) but ill be rollin out tubs in my new lab in no timeeeee!


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OfflinePsilocyBen17
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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
    #25144232 - 04/16/18 09:55 PM (3 years, 26 days ago)

btw my agar recipe was 20 g  agar to  20g potato flakes and a tablespoon of honey. I left the flakes chunky but will grind them up next time.


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OfflineLtLurker
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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
    #25144253 - 04/16/18 10:08 PM (3 years, 26 days ago)

20:20: how much water?

I'm usin 1g flakes:1.5g agar:100ml. A few times I boiled out too much water and the agar was hard. Myc grew slow and tomentose on it. Perhaps you're making your plates too stiff?


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OfflinePsilocyBen17
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Re: is weak myc on agar always a nute problem? [Re: LtLurker]
    #25144282 - 04/16/18 10:18 PM (3 years, 26 days ago)

I go off the ratio of 20g agar, 20g flakes, 1 litre of water. so for this batch I did 5g to 250ml. it was nice and soft but the growth still sucks. I never mix up my agar before pcing. I just put it in a wine bottle, swish it around, and pc it. Maybe i should start boiling n mixing thoroughly.


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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
    #25144288 - 04/16/18 10:21 PM (3 years, 26 days ago)

I'm sorry to bother you, but I see your on line and was wondering if you could answer a question I posted in my journal?  Thanks


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OfflineLtLurker
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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
    #25144296 - 04/16/18 10:25 PM (3 years, 26 days ago)

Your ratios sound right to me. The preferred way I seen is pouring hot water into a container with the nutes and agar and shake it up well before pc/pour.

Boiling works but I don't always get the agar melted evenly and have to stir it for like 15 minutes.

Either way, maybe your ingredients aren't getting mixed well enough based on your description?


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InvisibleMycolorado
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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
    #25144315 - 04/16/18 10:32 PM (3 years, 26 days ago)

Hey Ben!  Telephone brand blows.  It’s bulletproof at 2%....back it down to 1.25% for a stiffness you’re more accustomed to but don’t go lower.  Get new agar, I just decided to give it a second chance as I was out my go to eden brand and it really inhibits normal looking growth leading to a slow moving almost clear culture.  Like this:



I made xfers yesterday onto my regular agar and it’s already white and bouncing back.

Telephone brand =  :cuteshit:



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OfflinePsilocyBen17
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Re: is weak myc on agar always a nute problem? [Re: Mycolorado]
    #25144338 - 04/16/18 10:41 PM (3 years, 26 days ago)

hey Myc. I've used Golden Telephone in the past reliably, this time I am using some korean stuff (dont even know the brand cuz the package isn't english) and I'm having troubles. good to know your two cents on agar though.

How do you go about mixing yours? I'm really hoping a good mix and some grain soak water helps me out here. Its no fun getting back into something and not knowing if your doing it wrong :frown:


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InvisibleMycolorado
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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
    #25144360 - 04/16/18 10:53 PM (3 years, 26 days ago)

Ha, sorry, I totally misread the end of your post but still stand by my statement.  I’ve gone through several packs of it with a variety of cultures and never had good luck with it.  Anyway, I just add dry ingredients to the media bottle, top up to final volume with room temp tap and sterilize.  I like light malt extract or dog food at 2% for my nutrient sources.    I also like Stamets pda recipe.  Light malt extract is the easiest to use and best all around nute source for agar media and LC in my opinion.


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OfflinePsilocyBen17
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Re: is weak myc on agar always a nute problem? [Re: Mycolorado]
    #25144420 - 04/16/18 11:22 PM (3 years, 26 days ago)

when you say 2% do you mean 2% to the volume of water? can you explain the calculation?


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OfflineLtLurker
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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
    #25144431 - 04/16/18 11:30 PM (3 years, 26 days ago)

2% would be 2g per 100ml of water

1ml=1g i guess?


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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
    #25144434 - 04/16/18 11:31 PM (3 years, 26 days ago)

Yep.  A ml of water weighs a gram.  So for both agar and nutes the typical amount used is 2% of the total volume/weight.


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OfflinePsilocyBen17
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Re: is weak myc on agar always a nute problem? [Re: Mycolorado]
    #25144437 - 04/16/18 11:33 PM (3 years, 26 days ago)

got it thx myc.


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OfflineMooseShroom
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Re: is weak myc on agar always a nute problem? [Re: Mycolorado]
    #25144523 - 04/17/18 12:15 AM (3 years, 26 days ago)

Quote:

Mycolorado said:
Hey Ben!  Telephone brand blows.  It’s bulletproof at 2%....back it down to 1.25% for a stiffness you’re more accustomed to but don’t go lower.  Get new agar, I just decided to give it a second chance as I was out my go to eden brand and it really inhibits normal looking growth leading to a slow moving almost clear culture.  Like this:



I made xfers yesterday onto my regular agar and it’s already white and bouncing back.

Telephone brand =  :cuteshit:







I thought I was the only one who was having issues with it haha. I ran out of my usual and the only stuff I could find around here was telephone. I've been seeing really slow clear growth with it. Any brand recommendations?

Edit: Just saw you mentioned Eden brand. I'll take a look into that for my next batch.
Double Edit: Giving NOW brand a shot.


Edited by MooseShroom (04/17/18 02:05 PM)


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Re: is weak myc on agar always a nute problem? [Re: MooseShroom] * 1
    #25144562 - 04/17/18 01:07 AM (3 years, 26 days ago)

"Now" brand is a decent grocery store agar, as well.


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OfflineRomeoPapa
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Re: is weak myc on agar always a nute problem? [Re: hamloaf] * 1
    #25144637 - 04/17/18 02:27 AM (3 years, 26 days ago)

That's a bold statement to make saying telephone agar is shit and not worth using. I know you don't do pasty plates like I do, but I've had great success with telephone brand agar.

300ml water, 3 grams light dry malt extract from the home brew shop, and 3 grams of that shitty agar. Stir that shit up in a sauce pan on the stove for a bit and then pour into my mini rounds. This amount does 12 to 18 plates. However many i have on hand at the time. Cover and PC for 25.

That being said, I'm always down to try different brands of things. If it doesn't work out it goes in the garden usually, so nothing is lost!

Edit. I put my money where my mouth is. Eden agar was $12 per oz on eBay. Not gonna happen. Now agar was $8 for a 2 oz jar. I bought the now brand. We will see how it goes! Cheers everyone.


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Edited by RomeoPapa (04/17/18 02:35 AM)


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OfflineMooseShroom
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Re: is weak myc on agar always a nute problem? [Re: RomeoPapa]
    #25144644 - 04/17/18 02:38 AM (3 years, 26 days ago)

Quote:

RomeoPapa said:
That's a bold statement to make saying telephone agar is shit and not worth using. I know you don't do pasty plates like I do, but I've had great success with telephone brand agar.

300ml water, 3 grams light dry malt extract from the home brew shop, and 3 grams of that shitty agar. Stir that shit up in a sauce pan on the stove for a bit and then pour into my mini rounds. This amount does 12 to 18 plates. However many i have on hand at the time. Cover and PC for 25.

That being said, I'm always down to try different brands of things. If it doesn't work out it goes in the garden usually, so nothing is lost!




What led to the 1%/1% recipe? I've noticed that telephone brand is really stiff, so that could be the difference I've needed in the recipe compared to other brands.


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OfflineRomeoPapa
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Re: is weak myc on agar always a nute problem? [Re: MooseShroom]
    #25144719 - 04/17/18 03:53 AM (3 years, 26 days ago)

Nothing really. It's just easy to remember and I get good growth with it.



That's from the 12th and personally I think that's good growth. I don't consider myself a noob, but I am a total amature. And if my weights are off a little I don't sweat it.


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OfflinePsilocyBen17
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Re: is weak myc on agar always a nute problem? [Re: RomeoPapa]
    #25145093 - 04/17/18 10:45 AM (3 years, 26 days ago)

im going to do some experimenting and will update this thread as I do.

I’m also suprised to hear golden telephone has bad results. i used it with pasty plates....maybe the boil/mix step is important?

in my next 3 agar runs ill try

1. upping nutes with same mix (grain water)
2. golden telephone mix with same ratios
3. light malt extract


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Re: is weak myc on agar always a nute problem? [Re: PsilocyBen17]
    #25145101 - 04/17/18 10:49 AM (3 years, 26 days ago)

Good Luck Man. Hope it goes well. Mix thoroughly :smile:


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