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Offlinesteakfry
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Registered: 04/05/18
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Contams in me pasty plates, where did I go wrong?
    #25143381 - 04/16/18 01:27 PM (6 years, 8 months ago)

Made 8 pasty plates, but they are being contaminated helluh.
Where do most contams come from?
I used a SAB with an ISO soaked towel underneath to catch contams falling in SAB, also disinfected a small downstairs bathroom twice, wiped inside of SAB with ISO and disinfected, flame sterilized the spore syringe needle, even showering and putting on clean clothes, sanitized my gloves and even my face mask.
I PC'd the pasty plates loosely wrapped in tin-foil for 1 hour using an Instant Pot 6 qt, used the pressure cooker function which goes up to 15 psi, then cooks at about 10-11 psi, I compensated the pressure with more time in the PC.

Some theories:
-I did open the plates a couple times, but I saw contams when I did, so they got there before I did.
-I did not seal the pasty plates with tape,
-there was those bathroom vents like close to right above where I was inoculating.
-small bathroom, contams have less places to go, so there was a higher chance of them ending up in my SAB maybe?
-Micropore tape? Heard mixed stuff about it.
-Condensation inside plates? Also have heard mixed things, my impression is that it doesn't matter till it's excessive.
-Let em go in my bathroom, very exposed to open air as I leave my windows open a lot.

***Best and most promising ones weren't even PC'd, just threw a SAB on my bed and shot em up real quick, although a couple days until I am sure that they are healthy, although I doubt it.

Help improving my technique is appreciated  :takingnotes:  :takingnotes:




thas the bad stuff lol


--------------------
I really hate to ball on ya'll
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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
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Re: Contams in me pasty plates, where did I go wrong? [Re: steakfry]
    #25143621 - 04/16/18 03:19 PM (6 years, 8 months ago)

Technique doesn't happen overnight. Practice makes perfect. All of us work around a phenomenal amount of contamination just fine. that's the entire purpose of sterile technique.

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