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Amazon Shop: ½ Pint Jars, Agar, Autoclave

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Invisiblesporealchemy
ok


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Registered: 04/10/11
Posts: 155
Loc: mf mars my guy
Re: Easiest Liquid Culture for the incredibly lazy [Re: elasticaltiger]
    #25120376 - 04/07/18 11:49 AM (1 month, 17 days ago)

Quote:

elasticaltiger said:
Whenever a transfer piece bounces around on the agar I can see growth at every spot where the wedge hit the agar even though there was no visible mycelium on the spots.

I've been contamplating for a while meow whether it would be possible to clone a fruit just by rubbing a sterile swab on the inside. Never had the time to try it out though.




I've noticed this happening even tho i hate when a pin rolls around in a dish, but there are often multiple clean colonies.

And I'd bet that sterile swab would totally work and would like to try this also wow


--------------------
"Once your spawn jars are fully colonized, you can scratch your butt while you inoculate the coir if you want."
-RogerRabbit

( ͡° ͜ʖ ͡°)  anyone with an extra tamp or other sclerotia producer print laying around? ( ͡° ͜ʖ ͡°)


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Invisibleplurfekt
I like growing, nature and shit


Registered: 12/14/09
Posts: 1,741
Re: Easiest Liquid Culture for the incredibly lazy [Re: Pastywhyte]
    #25120726 - 04/07/18 02:41 PM (1 month, 17 days ago)

Quote:

Pastywhyte said:
Don't underestimate a cells ability to divide. I cloned a fruit once just by dragging a blade through the tissue, did not remove a visible amount of tissue. Then dragged that blade through agar and had growth.




I'm tryin it.

That's dope :thumbup:

BTW - thank you very much man; I'm now working with agar and have my first clean pasty plates colonizing; immediately went out and bought more mini rounds lol. :hug:


--------------------
"In all chaos there is a cosmos, In all disorder a secret order." ~Carl G. Jung M.D.

Obviously I'm an insane person and everything I post here is fictional, most likely a product of my over-active imagination. :shrug:

-Plurfekt

[gradient:#00ff00,#]Seeking medicinal/edible cultures, :pm: me for details.[/gradient]

:peace::heart::mushroom2::thumbup:


Edited by plurfekt (04/07/18 02:44 PM)


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OfflineDr. Freeman
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Registered: 03/27/18
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Re: Easiest Liquid Culture for the incredibly lazy [Re: plurfekt]
    #25136749 - 04/13/18 09:54 PM (1 month, 10 days ago)

Post edit:
I added the Grocery Store Agar Tek to my thread.
I think it would be a really good complement for any LC.


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OfflineGrimsweeper
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Re: Easiest Liquid Culture for the incredibly lazy [Re: Dr. Freeman]
    #25142099 - 04/15/18 11:04 PM (1 month, 8 days ago)

Doesn't appear that we can buy pre-filled sterile dextrose IV bags in the US. Just wondering your thoughts on filling an empty bag like this..

https://www.amazon.com/KangarooGravity-Feeding-Large-Bore-1000mL/dp/B00T5AFVDW/ref=sr_1_fkmr2_1?ie=UTF8&qid=1523846209&sr=8-1-fkmr2&keywords=iv+bag+autoclave

with a homemade LC then PC the whole thing.
Only thing I'm uncertain of is if there's an inoculation port on this bag or if you'd need something like this as well..

https://www.amazon.com/Baxter-IV-Set-Extra-Port/dp/B003VP2MR0/ref=pd_bxgy_328_img_2?_encoding=UTF8&pd_rd_i=B003VP2MR0&pd_rd_r=XS9SB7TVHHXTGPK64WFK&pd_rd_w=ymj9c&pd_rd_wg=s1jjW&psc=1&refRID=XS9SB7TVHHXTGPK64WFK


--------------------
When you clean a vacuum cleaner, you are a vacuum cleaner.
                                     


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OfflineDr. Freeman
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Re: Easiest Liquid Culture for the incredibly lazy [Re: Grimsweeper]
    #25143345 - 04/16/18 03:08 PM (1 month, 8 days ago)

I'm not sure the material of the bag would withstand the temperature of a PC.
And even if it did, a completely closed bag with boiling liquid doesn't seem like a good idea.
Probably the reason they use radiation to sterilize.

Post edit
I think making an IV bag defeats the purpose of buying pre-made.
Go with jars instead.


Edited by Dr. Freeman (04/16/18 03:11 PM)


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Amazon Shop: ½ Pint Jars, Agar, Autoclave

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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