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OfflineFungiCurious
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First agar contaminate?
    #25140696 - 04/15/18 10:18 AM (6 years, 8 months ago)

The growth here looks different than my other jars. You can see the healthy mycelium with some powdery white growth next to it. I'm thinking it's a contamination of some kind or just late forming mycelium. I'm hoping it's a contamination as I've been wanting to see the difference with my own eyes. The other 13 plates I made were contam free, which was kind of a bummer (I know I'm weird), but I was looking forward to the experience of cleaning up a contaminated specimen.

Thoughts?


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OfflineMycosperiment
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Re: First agar contaminate? [Re: FungiCurious]
    #25140795 - 04/15/18 11:13 AM (6 years, 8 months ago)

The powdery white is probably some kind of contamination or at least not something you want.  I'd say take some pieces of the good sectors and move them to fresh media.  If you want some practice, take some small pieces from the sectors at the 3 o'clock position and try not to get the powdery growth.

The rest looks great.  The part at the 11 o'clock position doesn't look as nice but still looks ok to me.  If the powdery growth starts changing color, it would be best not to try cleaning up the good parts especially if you have other good plates but you should be ok doing it unless it starts producing spores.

Be careful, working with agar can be very addictive.  Have fun.

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Offlinekanemush
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Re: First agar contaminate? [Re: Mycosperiment]
    #25140858 - 04/15/18 11:44 AM (6 years, 8 months ago)

contamination is usually easy to spot as the white dot will turn blue or green you can't miss it.

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Offlinebaddagger
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Re: First agar contaminate? [Re: kanemush]
    #25140866 - 04/15/18 11:48 AM (6 years, 8 months ago)

If your going to try to take a sample from that to get a clean culture going, try to grab from the 6 o'clock area, that area looks more constant growth wise and has some distance from that powdery spot.

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OfflineFungiCurious
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Re: First agar contaminate? [Re: baddagger]
    #25141629 - 04/15/18 05:34 PM (6 years, 8 months ago)

Quote:

Mycosperiment said:
The powdery white is probably some kind of contamination or at least not something you want.  I'd say take some pieces of the good sectors and move them to fresh media.  If you want some practice, take some small pieces from the sectors at the 3 o'clock position and try not to get the powdery growth.

The rest looks great.  The part at the 11 o'clock position doesn't look as nice but still looks ok to me.  If the powdery growth starts changing color, it would be best not to try cleaning up the good parts especially if you have other good plates but you should be ok doing it unless it starts producing spores.

Be careful, working with agar can be very addictive.  Have fun.



Quote:

kanemush said:
contamination is usually easy to spot as the white dot will turn blue or green you can't miss it.



Quote:

baddagger said:
If your going to try to take a sample from that to get a clean culture going, try to grab from the 6 o'clock area, that area looks more constant growth wise and has some distance from that powdery spot.




Thank you all for the replies. The quick response is much appreciated!!
I ended up transferring from the 3-4 and 6-7 o'clock positions as well as a chunk from the 1-2 o'clock position as well just for fun. :smile:

I agree with the addictive part, lol! I can't wait to get some actual petri dishes. I'm in a bit of an experimental state and needed something to do while waiting for my grain to colonize, I have two jars of just ms, and 6 with a ms/agar combo just for fun. I really want to get into cloning, but while also like to pay around with creating a master slant of very aggressive myc.

I've spent the last few months pouring every free hour into research on this site before even deciding on which ms syringe I wanted. While, having a successful first run following another persons tek using their experience is ideal for most, I'm not in this hobby to profit, definitely not in a hurry to harvest, and feel the best way to learn is to experiment and experience it for yourself.

Anyways, done with my rant, and thank you all once again! :smile:

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