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OfflinegamminEYE
Eeeya
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Registered: 05/29/16
Posts: 92
Last seen: 1 year, 10 months
Aged spore print questions
    #25139960 - 04/15/18 12:19 AM (5 years, 10 months ago)

I made some spore prints 5 months ago. Placed them on al foil, glass over the top. Cut out squares (of entire print) and wrapped them in folded alfoil. I stored these in the top of my cupboard.

3 days ago I took a scrape to go to agar. I've had no growth on the plate. I used a sterile syringe to add water on top of the spores morning and night.

Still nothing

This morning (12hours ago) I scraped another entire print and mixed it into a syringe with 1ml water (like spore syringe). In about an hour I planned on putting some drops on some other plates.

Are the prints dead? Am I doing something wrong?

I've gotten fresh prints to grow, this is my first time using a stored print.

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Offlinemisterswisher
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Registered: 04/07/18
Posts: 266
Last seen: 5 years, 1 month
Re: Aged spore print questions [Re: gamminEYE]
    #25139989 - 04/15/18 12:41 AM (5 years, 10 months ago)

5 months shouldn't be too long they should still be useful.

What temperature do you think it got up there?
idk maybe just give them a lil longer

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OfflinegamminEYE
Eeeya
Male

Registered: 05/29/16
Posts: 92
Last seen: 1 year, 10 months
Re: Aged spore print questions [Re: misterswisher]
    #25140005 - 04/15/18 12:51 AM (5 years, 10 months ago)

Probably a bit warmer than I'd like. I chose that cupboard because it was in the middle of the house and stayed the coolest. Temps got up to 46C during the peak of summer...

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