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InvisibleJosex
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Josex' Guide to No-pour Agar. * 31
    #25137693 - 04/14/18 08:36 AM (3 years, 7 months ago)

No-pours are Badass


Almost everybody starts off using no-pours, but it can get old after a while once you have some experience and your workload increases.
The people who after a while decide to switch to disposable petris usually do that for reasons such as:
  • To step up their agar game/increase their workload. 
  • To avoid wasting time on a tedious no-pour prep.
  • For ergonomics.
  • For easy storage and to make the most out of the space available.
  • To avoid wasting time on cleaning reusable containers.
  • For better visibility and to reduce/get rid of condensation.

This guide can't do anything about the last two, no-pours are no-pours after all, they have their pros and their cons.
But this guide will help you if you're in need of increasing your workload and doing a less painful prep.
I'll be showing you the containers I use for this, which are extremely easy to handle and make storage and clasification a breeze.

This guide is also meant for beginners, especially so. I'm sure you'll find here a couple of useful tricks and tips. :wink:

Note: the prep outlined in this guide can be done with any type of container suitable for agar, not only with the ones featured in this guide.



The Ideal Container for No-Pour Agar




Things to look for when choosing containers for no-pour:
  • Ideally, they have be round.
  • They have to be PP5 plastic, cup and lid.
  • Sturdy plastic that allows decent visibility.
  • Snap-on lid that closes fairly tight while allowing easy removal. Ideally the lid must open with an audible nice crack and the same crack should be heard when you close the lid. For that you need relatively thick and rigid plastic.
  • Simple design, avoid unnecessary grooves and marks.
  • Ideally, the bottom has to be flat.
  • Cup and lid have to be clear.
  • 4oz (100ml) max. capacity.
  • They have to be cheap, so you can buy them in bulk.
  • They have to be stackable.
  • They have to be ergonomic and easy to handle.

Things to avoid:
  • Bulky or heavy containers, it's all about those ergonomics.
  • Flimsy or thin plastic.
  • Glass: glass containers are not as easy to handle as plastic containers. Handling glass with alcohol-soaked gloves can be a slippery business. Also, you just can't fit as many in the PC.
  • Screw-tops: again, for ergonomic reasons.
  • Raised bottoms and unnecessary marks and grooves.
  • Hinged snap-on lids.
  • Lids that aren't clear.
  • Short sides.

The lists above are not set in stone, but have their basis on personal experience after having tried a variety of different containers.
I prefer to use what overall makes my life easier; prep, culture work, clasification, storage and cleaning.

A little disclaimer, though: failling to follow the above guidelines could potentially lead to contams or other issues along the line, as the great majority of containers on the market simply aren't good for this. 



FINDING THE RIGHT CONTAINERS

These are the containers I use, which meet all the requirements mentioned above. They are durable and quite thick and sturdy, while allowing decent visibility.
Some pics, although in real life visibility is better:



Finding appropriate deli containers for agar on the internet can be a bit of a challenge, but the struggle is totally worth it if you manage to find the right ones.
The following is a list of things you should do and take into account before purchasing:
  • You will need to contact the vendor and ask them if both cup and lid are PP5. If you don't specify they can tell you their containers are PP5 when in fact only the cup is and shit happens...

  • Very often, vendors don't specify in the item's description that their containers are polypropylene (PP5) even if they are, but if the description says something like "dishwasher, microwave and freezer safe" there's a good chance those containers are indeed PP5.
  • Sometimes, the pictures of the containers the vendor has on display don't correspond to reality. You need to bug the fucker and ask them for some real pics of the container and even ask them for a pic of the inside of the cup to ensure the bottom is flat. They'll usually acquiesce to this request if you make them swallow a little white lie like, "I'm the owner of a fast food restaurant and need thousands of these containers for my sauces...". You'll make 'em your bitch. :hehehe:
  • Again, you need sturdy plastic and a simple design.

    And avoid:

  • Whatever you do, avoid the temptation of getting what can seem super good deals on plastic containers. Even good quality containers should be cheap, but trust me, super cheap can cost you dearly.
  • Buy them in bulk (500-1000 containers). It'll be cheaper this way and you'll have containers for life. There's a pretty good chance you'll regret just getting like 100. A very lame thing to do.
  • When the box of containers you ordered arrives, carefully open the box without damaging anything, carefully open a bag of cups and another bag of lids and make sure your overall impression is good and corresponds with the descriptions of the ideal container you've seen here.
    If you find something iffy you can just return the box in its original state, which is a prerequisite in most return policies.


Modifying the Containers


For this tek, we're going to be making a hole through the side of the containers, not through the lid. There is a few advantages to doing this, but the most important one is that it allows you get away with no wrapping the containers in foil or using paper towels to protect the filter during the pc cycle. More about this later.

A small hole will help in keeping the plates fresh for longer without drying out. There's absolutely no reason to make a big hole.

The only real purpose of adding a filter is to prevent the plastic containers from warping during the pc cycle, and for that a tiny hole will do. 
The filter is not really intended to enable gas exchange, since there's more than enough air in a container to support healthy growth even if there was no filter.

You can use whatever to make the holes; a small drill bit, a hot nail, a punch, a small screwdriver...
I personally like to melt a slit through the side using a small flat-head screwdriver, instead of drilling.



Simply take a torch and flame the tip of the screwdriver. Flame the screwdriver just enough to allow you to make the hole with ease. You can make several holes before you have to flame again.
Use only the tip of the screwdriver to make the hole.

Flaming red hot can make a mess of melted plastic around the edges of the hole. This can even happen in a lesser degree without flaming red hot, but you can easily scratch off the melted plastic around the hole with your fingernail.

This is the result.



Now cover the holes with 2 or 3 layers of MP tape. I personally like to add 3 layers of tape.



The combination of a small hole and several layers of tape allows you to keep the plates fresh for very long.
They'll be good for quite more than a month at room temp.
This means you can prepare a great number of plates one day and have agar plates handy and ready to use for many days.

3 layers of MP tape will last in good condition for many PC cycles. If at any point you see the tape has deteriorated, you can simply add another layer of tape for the next PC cycle, without removing the old ones.
You can stack layers of MP tape no problem.
The max I've done was 6 layers and that lasted for many pc cycles.


BEAST MODE! Let's Make some No-Pours

A no-pour prep doesn't necessarily have to be so tedious or rob you of a lot of your precious time. We're going to keep things simple and reasonably painless.

One of the key advantages of using the type of container featured in this guide is that it allows to make the most out of the space in your pressure cooker. You can fit more of them in the PC than when using most any other type of containers. I can fit 120 plates in my PC and could fit more if I wanted.

This is obviously good if you do lots of agar but it doesn't matter if you don't, because as I mentioned earlier, you can keep the plates stored for quite a long time. Having blank plates handy whenever you need them is a beautiful thing.
You can keep blank plates unused for more than 2 months stored in a tote at room temp and they'll be as good as fresh out of the PC if you follow the simple steps in this guide.


ONE CUP, ONE LID

You have made the holes in your containers and applied the 3 layers of tape.
The first step for the agar prep is laying the empty cups in an orderly fashion on a table or kitchen counter.



This is self-obvious and it wouldn't need any explaining at all except for one important thing that I think deserves a few lines.
In order to extend the life of your containers considerably, it's important that you always use the same lid for the same cup.

The containers featured in this guide will last countless PC cycles in good condition (some of mine are more than 2 years old without showing any signs of wear), but only if you take the precaution of always using the same lid for the same cup. If you don't, you'll mess them up fast.

Lids and cups that have had different numbers of cycles won't fit well. The lids may close too tight or too loose, so loose they won't offer any real protection. One lid, one cup, and you will never have this problem.

You can go two ways about this.

A simple thing you can do when you clean a plate after use is immediately put the same lid on and store the empty plate with the lid on for later use.
I concede that this can be a bit of a pain, but trust me, the nuisance of taking this precaution can't begin to compare with the frustration of having your containers all messed up from the very beginning.

The following are some diagrams to help you understand what I do. Of course, you don't need to do this when you are using cups and lids that are new out of the box, only when they have had at least one PC cycle.

You can arrange the empty plates like this:



And once you finish pouring them you do this:



The second thing you can do is marking each cup and lid with a number using a permanent marker, so the lid number 1 will always go with the cup number 1 and so on.
The permanent marker will cure after a PC cycle, meaning that it's going to be impossible to remove the labels even with alcohol, they're going to stay there forever like a tattoo.
The advantage of doing this is that you can store the lids and cups separately after use and since they're stackable they'll take very little space.
One of the drawbacks is that it can be a nuisance trying to find the lid that matches the cup among so many cups and lids, and also, that maybe you'd rather have your containers not marked and in pristine condition.
So do whatever way you prefer.


SPECIAL SHOUT OUT TO LIGHT MALT EXTRACT (LME)

I'll admit I'm partial to LME. There are several good agar recipes, but LME has a few advantages over the rest:
  • Super easy prep. All agar preps are easy but MEA is just ridiculous stuff.
  • Easy to sterilize. LME is an inherently clean media, which allows you to get away with a short PC cycle.
  • Super cheap. A bag of 500 grams of LME could perfectly outlast you and won't go bad if stored properly.
  • Perfectly clear media without any sediment. Good for shining a light through the bottom of the container to let you see growth patterns from a different perspective.
  • All in all, awesome media. Mycelium just loves to grow pretty on MEA. :cool:


There are no excuses for not using LME, it's also amazing for LC's. It's very easy to find online, fairly cheap and it pays for itself considering a bag of 500g is fucking infinite.

You have to reseal the bag with as little air as possible after use. Close the bag very well and use a peg, then put the bag inside a ziploc bag and store it in a dry and cool place.



LME is very hygroscopic, meaning that is able to absorb moisture from the environment like a madafokin bitch, which leads to clumping and solidifying to a rock-like consistency. Taking those simple precautions will take care of that.


PREPPING THE AGAR

For transfers, anywhere from 1,5% to 2% agar strength is good. The same goes for nute strength.
I personally like 1,5% agar strength and 1,5% nutes, just because it still works just fine and you save up a little on materials in the long run.

With 16,5 grams of agar, 16,5 grams of LME and 1100 ml of water I can make 120 plates.

Let's get to it:
  • Tare the cointaner and weigh out the agar and the LME separately.

  • Pour the amount of water you're going to use into a measuring jar. I use 1100ml of distilled water for 120 plates (you can also use tap water).
  • Throw the dry ingredients into a pot, add a few drops of food color (optional) and then pour the water slowly into the pot, being careful not to pour the water directly onto the dry powdered ingredients.
  • Put the pot on the stove at medium-high heat and stir constantly from the start until the mixture has dissolved completely.
  • Once the dry ingredients have dissolved, stir only occasionaly until the mix is ready.
    Some people like to take the pot out of the stove when the mix starts to simmer, but that isn't really necessary. You'll know when it's ready when the mix starts to get nice and thick.
  • Once it's ready, pour the agar mix from the pot into a measuring jar. The mix will form some foam on the surface so I like to take what I can with a spoon, although that's just me and my OCD.
  • A little of water may have evaporated off, pour enough water (cold is fine) to replenish what was lost.

POURING PLATES

Very little to say about this, just pour them plates.



I love to pour thin. Just pour a little, enough to cover the bottom.
If you think you've poured very little you've poured enough. :thumbup:



Pouring thin is just elegantly gangsta, avoids wasting agar unnecessarily and it also makes it easier to cut a wedge.

Some people like to pour rather thick because it helps them to get the wedge off the blade by making a cut on the receiving plate.
But that's just a crutch and an excuse for not wanting to get better at what can be the most important little skill to master when it comes to agar work, namely, scooping up the wedge just right so that you can get it off easily and smoothly by contact with the agar surface alone.


Loading the Plates in the PC

When you finish pouring the plates, you need to let them solidify and then put the lids on as explained in the section "one cup, one lid".



It's important that you let the agar solidify before loading the plates in the PC.
When the plates are cooled and solid it's much easier to place them in the PC without worrying about splashing the agar up the sides and under the lid.
By the time you finish pouring the last plate, most likely half the batch will have solidified already.

While you wait for the rest of the plates to solidify you can start preparing the PC:
  • Add a dash of white vinegar to prevent the hard water deposits from staining the containers.
  • The plates need to be elevated above the water level. Use something to elevate the plates, get creative.

  • If your PC comes with the same racks you see above, cover the top rack with foil for better stability.

  • Pour some hot tap water into the PC.

If you've never operated a pressure cooker before, you should read this first.

Now let's put the plates in the PC:
  • The first layer of plates goes directly on top of the PC rack, of course.

  • Cover this layer of plates with 2 sheets of foil. Tuck in the foil around the edges, no need to be thorough, just enough to make the foil flat and without any bumps for the next layer of plates.

  • Place the second layer of plates on top of the first.

  • Rinse and repeat until your PC is crammed full of plates.


Put the lid on and turn the heat on high.
Vent the PC for 10 minutes counting from the moment you start to see steam coming out of the vent pipe.

Now, hear me out.
Seriously, turn the heat on high and keep that shit up until the PC comes up to pressure. Disregard all you've read about bringing the PC up to pressure slowly in order to reduce condensation. More about this later.

Once the PC reaches 15 PSI turn the heat as low as possible, enough to maintain the desired pressure.

Depending on the media you're using, you can sterilize the plates from 30 to 45 minutes at 15 PSI.
If you use LME you can get away with as little as 20 minutes at 15 PSI.


Cooling the Plates in Open Air and Storage


Let me start by pointing out that leaving the plates in the PC overnight is truly terrible practice.
This is hardly an opinion, looks more like a fact.
There are no benefits to doing this whatsoever, but you may indeed encounter some issues if you do so.

For whatever reason, beginners tend to fear contamination if they take the plates out of the PC while they're still hot. Rest assured, that won't happen.
Then there's the ones that leave the plates in the PC overnight just out of sheer laziness. :smbfacepalm:

We often see people complaining about some issues that can be traced back to leaving the plates in the PC overnight, like:
  • Wet filters.
  • Uneven condensation in the plates and water pooling on the agar surface.
  • And what about this:



Taking the plates out of the PC while they're still hot is a bit of a pain, I'll grant you that. You have to take them out carefully as to avoid splashing the still liquid media on the filter and under the lid.
But in my opinion, it's just something you should do if you have any kind of standards.

When you open the PC, you're going to find some pooling water on the first sheet of foil, of course.
When taking the first piece of foil it's going to be impossible to prevent part of this water from sliding down onto the next sheet of foil and getting some water on a couple of lids in the process, big deal... just wipe those lids with a paper towel.
You're also going to find that the filters are literally bone dry.

Carefully put the plates on a flat surface so the agar sets nice and level, I put them on the kitchen counter.
You can use gloves to protect your hands from the heat if you happen to have a sensitive skin (honestly? I never use them).



Wait 15-20 minutes for the agar to solidify and then store them in a plastic tote.



                  :cookiemonster:

There you go, now you have plenty of agar plates for weeks to come. 
Put the lid on and write down the date on the tote if you wish.


Technique


For transfers, working with any type of pp5 containers becomes super easy if you keep them upside-down, like you can see in this video where I'm doing a simulation, which, by the way, is something I'd recommend you do often in order to get better and gain confidence if you happen to be a beginner.

Getting the wedge stuck on the blade is very frustrating, so it's important you learn how to scoop up the wedge with the tip of the blade so it comes off smoothly on the receiving plate when you transfer it.



Working with the plates in an upright position can be rather awkward by comparison, mainly because the tall sides of the plastic containers won't let you have a clear view of the culture. Also, the tall sides will force you to position the scalpel at an awkward angle to take a wedge, forcing you to place your hand dangerously near over the plate.

Finally, working with the plates upside-down is a good way to lower the risk of anything nasty landing on the media.
You'll also be more comfortable, and this alone is something that often translates to a higher success rate.


SETTING UP THE SAB

Simple does it, click here.

I do like to place a towel on the table which I previously soaked in the shower and then wrung out well. The towel will keep the SAB from moving around and it will absorb the water from misting.

Use something like a metal rack to keep the plates above the towel. You can also put an alcohol soaked paper towel on the rack (after misting) to prevent the plates from sliding around.

Personally, I only introduce in the SAB 2 plates at a time, the donor and the receiving, which get wiped down with 70% alcohol right before introducing them in the SAB. I just hate to clutter up the SAB, especially for long and tedious agar sessions, so one day I decided to do this, liked it and never looked back, no issues whatsoever. But by any means, do what gives you the most peace of mind.

Once I transfer I take both plates out of the SAB and never label right away. Instead I stack the receiving plate/s on top of the donor plate outside the SAB. Like in the pic. The donor plates are the ones that have labels and the ones right on top with no labels are the receiving plates from that donor. I label them when I'm done with the SAB session.




LABELLING

I used to use a permanent marker to label the plates, fuck that. It's such a hassle having to remove the labels with alcohol. Currently I do this:



These stickers are dirt cheap and easy to remove aftewards.

You can keep your plates on a shelf while they colonize, or in a tote. Since they're stackable they take very little space and you can classify them easily.


CLEANING THE PLATES AFTER USE

Due to the fact that these containers are rather rigid, they won't allow to squeeze out the agar puck, like you can do with glad mini rounds for instance. Instead, I take my scalpel and detach the puck from the very edge using the tip of the blade. The puck comes out easy and quick and weird enough, I find it to be a strangely satisfying task. :lol:

Then I wipe down the cup and the lid with a dry paper towel and that's it, no washing necessary. Then, snap the lid on and store the empty containers like that for later use.



Bullshit List

You've probably found in this guide some things that may contradict some info you may have read on the boards.
Unfortunately, some of the stuff you read comes from people with very little or zero actual experience.
This is a public forum and everyone is entitled to express their ideas and opinions even if they are wrong, so you should take this into account and exercise caution when presented with new info.

The following is a list of things you should never do that get repeated quite often. Most of them regarding condensation, as if it was the end of the world. Take a look, though I'm pretty sure I'm missing out some good ones:
  • Making a big hole in the containers:
    With the idea of making condensation dissipate faster. This is asking for trouble, the bigger the hole, the greater the possibility of filter failure. You can also keep them fresh for way longer if you only make a tiny hole.

  • Letting the agar cool in the pot before pouring:
    Yep, with the idea of reducing condension. This is ridiculous, pour it hot.

  • Waiting for the condensation in the plates to go after pouring:
    It won't get you less condensation after the cycle.

  • Wrapping in foil to reduce condensation:
    You'll end up with the same condensation. :shrug:

  • Bringing the pc up to pressure very slowly to reduce condensation:
    It just won't, turn the burner all the way up until you reach pressure.

  • Bringing the temp down slowly (again, to reduce condensation):
    It won't help a bit and the gas should be set low anyway after bringing the pc up to pressure.

  • Keeping the plates in the pc overnight:
    Horrible practice, as we saw before. Take them out still hot so the agar sets level on a table.

  • Finger flicking the plates to get the condensation off of the sides and lid:
    Terrible, all that water will pool on the agar surface.

  • Using a paper towel to wipe the condensation inside the containers:
    They open the containers in the SAB and do this. Real talk... :facepalm:

  • Keeping unused plates in the fridge:
    This is awful. First off, there's no need to do this because you can store them at room temp for quite long. Second, if there's bacteria present due to an insufficient cycle you want it grow so you know not to use them.

  • Keeping the plates upside-down while they colonize:
    Water from condensation and nute-rich liquid will go to the lid where it's more likely there'll be mold spores waiting to germinate or even bacteria.

  • Condensation is the worst, can cause contamination and should be avoided at all costs:
    Condensation is not an issue whatsoever, just let it be and pretend it's not there. Anything you do to try and get rid of it will likely be a noob move.

  • Using cling wrap to wrap the plates:
    If you need to do that because you're getting contamination during colonization then you're most likely using containers that aren't good for this. A good container should not contaminate under normal circumstances without using cling wrap. Also, wrapping them with foil instead is equally useless.


:cheers:


Edited by Josex (07/08/20 12:45 AM)


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OfflineFeedYourMind
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25137713 - 04/14/18 08:49 AM (3 years, 7 months ago)

Now that’s a freaking tek. So much great info. I am definitely looking into this as I just got all my supplies in to up my agar game. Impeccable timing, really. :congrats:


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InvisibleJHOVA
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Re: Josex' Guide to No-pour Agar. [Re: FeedYourMind]
    #25137799 - 04/14/18 09:49 AM (3 years, 7 months ago)

:vibin::vibin::vibin:

What a great fuckin tek Josex. I can atest to your list of minsnomers. Leaving plates in a PC overnight is terrible practice as you proved here.:wizard:

If you live in the americas is isn’t worth the shipping to get anthing less than 500 plates.


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Re: Josex' Guide to No-pour Agar. [Re: FeedYourMind]
    #25137803 - 04/14/18 09:50 AM (3 years, 7 months ago)

Wow great same lme has I use,your right about it turning into bricks of pouch left open.


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InvisibleJosex
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Re: Josex' Guide to No-pour Agar. [Re: Sutty86]
    #25137808 - 04/14/18 09:53 AM (3 years, 7 months ago)

:goodday:

Hey Jhova, thanks man. BTW, how much are the shipping costs for you for 500 containers? I'm very curious, hope it isn't much.


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OfflineCHOOS
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25137820 - 04/14/18 10:02 AM (3 years, 7 months ago)

What a write-up!  :eek:

i thought the side holes were done so you could stack them in the PC.
But then you didn't.
Why did you choose the side instead of the lid for the holes?


Edited by CHOOS (04/14/18 10:05 AM)


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InvisibleJosex
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Re: Josex' Guide to No-pour Agar. [Re: CHOOS]
    #25137859 - 04/14/18 10:22 AM (3 years, 7 months ago)

Quote:

CHOOS said:
What a write-up!  :eek:

i thought the side holes were done so you could stack them in the PC.
But then you didn't.
Why did you choose the side instead of the lid for the holes?




Good question. If you stack them without the sheets of foil they'll stick together in the pressure cooker. Creates a bit of vacuum between the lid of the container below and the bottom the container above, at least it happens with these containers, and you can make a mess when you separate them while they're still hot and the agar is still liquid. The sheets of foil also prevent water from gettin on the lids.

Making the hole through the sides is to avoid getting the filters wet, they'll come out bone dry after the cycle every time if you do what's explained in the OP.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25137884 - 04/14/18 10:37 AM (3 years, 7 months ago)

Nice bullshit list.

People come up with some really stupid ideas for sure.

The first one pouring while hot/warm vs 120F only works for pour plates.
Idk why people think it would make sence or any difference for plates that still need to get pressure cooked.


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Re: Josex' Guide to No-pour Agar. [Re: bodhisatta]
    #25137926 - 04/14/18 11:04 AM (3 years, 7 months ago)

Heeyyyy, i use to finger flick my no pours. Sometimes that pooled water is useful for old spore prints:oldman:


But the bullshit list should be mandatory for future teks. Awesome write up dood.
:manofapproval:


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Re: Josex' Guide to No-pour Agar. [Re: mushboy]
    #25137935 - 04/14/18 11:09 AM (3 years, 7 months ago)

Very nice write up man. Thanks.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25138114 - 04/14/18 12:33 PM (3 years, 7 months ago)

What do you mean by "wiping the walls of the container with a paper towel while in the SAB. :facepalm:"?

I like hole hole through side of container.  Innovative.


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Re: Josex' Guide to No-pour Agar. [Re: LtLurker]
    #25138124 - 04/14/18 12:39 PM (3 years, 7 months ago)

Yey the bullshit list haha
I'd be happy if it helps get a load off of the Pasty plates thread, most of those whacky ideas come from there, people asking questions and others giving that type of advice which ain't advice.

Thanks doods!


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Re: Josex' Guide to No-pour Agar. [Re: hamloaf]
    #25138129 - 04/14/18 12:41 PM (3 years, 7 months ago)

Quote:

hamloaf said:
What do you mean by "wiping the walls of the container with a paper towel while in the SAB. :facepalm:"?

I like hole hole through side of container.  Innovative.




Actually wiping the sides, I should edit that. Yeah as it sounds, never read anything like that Ham? They do that to get rid of condensation on the sides. :brilliant:


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Re: Josex' Guide to No-pour Agar. [Re: Josex] * 1
    #25138137 - 04/14/18 12:48 PM (3 years, 7 months ago)

:nodofunderstanding:

Never read anything like that, but it does not sound like good practice.  Condensation is a non-issue.  It's a very very remote possibility of a vector, but nothing to be concerned about.  Condensation will clear up if plates are kept at a steady enough temperature.  Mild temperature swings actually aid in causing gasses to exchange. 

Nice one, Jo!  :thumbup:


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Edited by hamloaf (04/14/18 01:43 PM)


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25138225 - 04/14/18 01:32 PM (3 years, 7 months ago)

Quote:

Josex said:
:goodday:

Hey Jhova, thanks man. BTW, how much are the shipping costs for you for 500 containers? I'm very curious, hope it isn't much.




it's expensive for me. i recommend the uk ebay site cuz i cant read spanish for shit and its the same parent company "General Stores LTD".

29.99 pound sterlings - 500 containers
26.53 pound sterling for the only option of shipping
no import charges to 'Merica
total 56.52 pound sterlings = $80.50 FREEDOM DOLLARS shipped

ninja edit:
If anyone has any vendors of similar type pp5 containers in the US/Americas (ebay) that are awesome please do list them here!


Edited by JHOVA (04/14/18 01:37 PM)


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Re: Josex' Guide to No-pour Agar. [Re: JHOVA]
    #25138263 - 04/14/18 01:48 PM (3 years, 7 months ago)

@Jhova, I wouldn't buy any less than 500 containers so that's what I recommended in the OP, but you can also get boxes of 100 containers from the same vendor.

$80.50 FREEDOM DOLLARS shipped : 500 containers = 0,161 Freedom Dollars per container.

Looks :lookslucrative: to me.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25138300 - 04/14/18 02:04 PM (3 years, 7 months ago)

i didnt see 100 container option from the uk store. they have 2 things they sell on the uk store and that is the 500 and 200. brought to you by Josex LTD:hehehe:
they do have 200 for 13#'s.
13+27 = 40# = $56.97.

even 200 is a good deal now that i priced it out. you could make a batch once a month and the third month would already be cheaper than disposable petris and you don't raise suspicion from fucktard landlords or local law enforcement.


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Re: Josex' Guide to No-pour Agar. [Re: JHOVA]
    #25138306 - 04/14/18 02:12 PM (3 years, 7 months ago)

:kimjongil:
finnaly. Another good & patiently write-up for the community.

I think ive locally sourced some good cups to replace my old ones.

Only problem i see with pp5 the more thick & sturdy the less the transparency..
And my new ones are simple sturdy, hard & thick as glass jars.

Can you still press and do tiger drop with your containers brother josex?


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Edited by pacmanbreed (04/14/18 02:20 PM)


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25138315 - 04/14/18 02:17 PM (3 years, 7 months ago)

I found this thread using the search engine, since I was looking for a European alternative for those glad mini rounds and other American brand storage containers. Just realized this thread is hot off the press, so this was posted at the perfect time for me. Thanks a lot!


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Re: Josex' Guide to No-pour Agar. [Re: pacmanbreed] * 1
    #25138337 - 04/14/18 02:31 PM (3 years, 7 months ago)

Quote:

pacmanbreed said:
Can you still press and do tiger drop with your containers brother josex?




No you can't. Who wants to use agar these days anyway, get your LC game tight :hehehe:
Back when I still used agar I preferred good ol wedge to grain, even when using glad mini rounds. Not a fan of the drop tbh.


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25138354 - 04/14/18 02:36 PM (3 years, 7 months ago)

Great write up jo! Killer work brother.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25138357 - 04/14/18 02:38 PM (3 years, 7 months ago)

Quote:

Josex said:
No you can't. Who wants to use agar these days anyway, get your LC game tight :hehehe:
Back when I still used agar I preferred good ol wedge to grain, even when using glad mini rounds. Not a fan of the drop tbh.




Thanks for that pointers brother. Having these feeling ive found some suitable local replacement for my containers to step-up my biopsy lc game. Will also try your modded lc jars in our moldy apartment. :rockon:


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Edited by pacmanbreed (04/14/18 02:39 PM)


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Re: Josex' Guide to No-pour Agar. [Re: pacmanbreed]
    #25138374 - 04/14/18 02:45 PM (3 years, 7 months ago)

You should include glad mini rounds as an approved container


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Re: Josex' Guide to No-pour Agar. [Re: Just_A_Noob]
    #25138376 - 04/14/18 02:48 PM (3 years, 7 months ago)

It's an approved container already :shrug: nothing I can say will make it any better. I'm just sharing what I do and I had good times with glad mini rounds as well when I started.

Besides, I'm the only poor fucker afaik that shelled out good money to have glad mini rounds imported from 'Merica. People from Europe need an alternative.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25138385 - 04/14/18 02:52 PM (3 years, 7 months ago)

:thumbup:, I hear you, I've just seen a couple posts where people have linked this Tek asking where they can get containers locally in US.

Nice write-up btw. :rockon:


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25138386 - 04/14/18 02:52 PM (3 years, 7 months ago)

:coolpost:

Been using similar containers for a while now when doing no pours. Although I prefer actual petris and don't mind pouring.

Sick write up dude.


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Re: Josex' Guide to No-pour Agar. [Re: Just_A_Noob]
    #25138418 - 04/14/18 03:09 PM (3 years, 7 months ago)

Quote:

Just_A_Noob said:
:thumbup:, I hear you, I've just seen a couple posts where people have linked this Tek asking where they can get containers locally in US.

Nice write-up btw. :rockon:





I really wish someone could find containers similar to these in the US so people who like this type of container could benefit as well in the US. The problem is that I've read several posts from people over a long period of time (including Locn9ne) that said they couldn't find nice deli containers in the US, they're usually much bigger or have characteristics that make them inappropriate.

Jhova posted the total shipped for him for a box of 500.

Quote:

JHOVA said:
total 56.52 pound sterlings = $80.50 FREEDOM DOLLARS shipped




That's 0,161 USD shipped per container. People pay that for petris every time and I don't know the price of glad mini rounds there but I doubt it's that cheap per unit?


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25138425 - 04/14/18 03:12 PM (3 years, 7 months ago)

It's not cheaper but available same day


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Re: Josex' Guide to No-pour Agar. [Re: Just_A_Noob]
    #25138578 - 04/14/18 04:17 PM (3 years, 7 months ago)

nailed it. I dont even worry about the condensation. it usually goes away after a week anyways.


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Re: Josex' Guide to No-pour Agar. [Re: Ziran]
    #25138650 - 04/14/18 04:45 PM (3 years, 7 months ago)

I bought 50 deli containers from ebay @ 5.50 usd for 50. 1 month to come from china but im not in a hurry to use agar :laugh:


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Re: Josex' Guide to No-pour Agar. [Re: roses4lg] * 1
    #25139030 - 04/14/18 06:58 PM (3 years, 7 months ago)

Solid tek. I'm a fan of the containers that are used and they weren't that expensive for me to ship. Overall, I paid about $40 for 200 containers. There was a bit of a learning curve on using them, but I'd say it was well worth it. For 500 it's about $80 from the looks of it, but I just wanted to get my feet wet before making the jump.

For a 23q presto I've found that I can fit about 12 containers per level, and can easily fit 50 containers into one cycle (haven't tried to put more yet). I've raised the trivet with foil, but I think in future cycles I'll use jar rings, trivet, jar rings, second trivet.

Here's a picture of some that I completed with these containers.


I can also confirm that the visibility is much better in person. When trying to take a picture of it the flash on the camera shows up too much. Haven't personally found a good way to photograph them otherwise.

On my containers I like to number the lid and container themselves because otherwise I'll forget which one goes to which.

Kickass tek Josex :super:

Edit: Using the double jar rings and trivet method I can comfortably fit 60 of these containers in a 23q presto (5 layers of 12).


Edited by MooseShroom (04/18/18 02:21 AM)


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Re: Josex' Guide to No-pour Agar. [Re: MooseShroom]
    #25139112 - 04/14/18 07:21 PM (3 years, 7 months ago)

:rockon:


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25139442 - 04/14/18 09:09 PM (3 years, 7 months ago)

Thanks for the great tek man. I'll definitely have to order some of those containers!


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25139831 - 04/15/18 12:28 AM (3 years, 7 months ago)

Finally! A well formatted tek! Great job.

Quote:

Josex said:
but only if you take the precaution of always using the same lid for the same cup. If you don't, you'll mess them up





What's the significance here? Why do we have to have monogamous lids?


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Re: Josex' Guide to No-pour Agar. [Re: tokey666] * 1
    #25140287 - 04/15/18 08:44 AM (3 years, 7 months ago)

Lids and cups that have had different numbers of pc cycles won't fit well. The lid might snap too loose or too tight on the cup, and by loose I mean dangerously loose, without being able to offer any kind of protection anymore. Imagine you just finished pouring and need to put the lids and they're all messed up, some close super tight, some just rest loosely on top, that's a bitch. The bevel on the cup where the lid goes is thin, unlike mini rounds for instance, so if the lid doesn't close tight you are guaranteed to see mold.
Same lid, same cup, and you'll never have this problem.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25141868 - 04/15/18 09:16 PM (3 years, 7 months ago)

Very nice write up.


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Re: Josex' Guide to No-pour Agar. [Re: woodpusher420]
    #25146530 - 04/17/18 09:53 PM (3 years, 7 months ago)

Thank you for this write up, Dam wish i saw this eailer...

I got some sqaureish containers, I left them overnight, etc and my filter (ontop) is asboultely drenched, It was 2 peices of micorpore and that is stuffed aslong with the paper towl that was stuck to it ontop...

Should i bother trying these containers? Just placing another peice of tape ontop, Let them dry out for a few hours in my sab & try them?

I carefully 1 by 1 opened them and poured out the excess water, was only a few drops some a good 10 or so, My agar looks like it didn't set as well as it could of aswell. I know what mistakes i have done now thanks to this writeup!!!

Honestly, Should i try inccoulate these containers? I am tempted to move onto pouring i think i'd enjoy it better


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Edited by mindblowing (04/17/18 09:56 PM)


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Re: Josex' Guide to No-pour Agar. [Re: mindblowing]
    #25147043 - 04/18/18 01:50 AM (3 years, 7 months ago)

Glad it could be of help.
I'll be blunt man, those containers look terrible for agar, it's not only the shape they have and the huge size, but also those clamps and the short sides.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25147299 - 04/18/18 06:31 AM (3 years, 7 months ago)

Quote:

Josex said:
Quote:

Just_A_Noob said:
:thumbup:, I hear you, I've just seen a couple posts where people have linked this Tek asking where they can get containers locally in US.

Nice write-up btw. :rockon:





I really wish someone could find containers similar to these in the US so people who like this type of container could benefit as well in the US. The problem is that I've read several posts from people over a long period of time (including Locn9ne) that said they couldn't find nice deli containers in the US, they're usually much bigger or have characteristics that make them inappropriate.

Jhova posted the total shipped for him for a box of 500.

Quote:

JHOVA said:
total 56.52 pound sterlings = $80.50 FREEDOM DOLLARS shipped




That's 0,161 USD shipped per container. People pay that for petris every time and I don't know the price of glad mini rounds there but I doubt it's that cheap per unit?




glad mini rounds are 2.50 usd which is 31.25 cents per container

500 x 31.25 is 156.25$ so the ones used in this tek are cheaper


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Re: Josex' Guide to No-pour Agar. [Re: thelanzii]
    #25147850 - 04/18/18 01:19 PM (3 years, 7 months ago)

I have to admit he's right in a sense though. If someone is just getting started with the hobby maybe it's not such a good idea to invest in a box of 500 containers, no matter how cheap they're individually, so getting what they can find locally sounds about right to get you started.


Edited by Josex (04/21/18 10:36 PM)


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25149048 - 04/18/18 09:02 PM (3 years, 7 months ago)

Thank you for the detail write-up


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Re: Josex' Guide to No-pour Agar. [Re: BigGameHunter]
    #25149992 - 04/19/18 05:48 AM (3 years, 7 months ago)

Bad news. I've a friend here in Spain I'm teaching to grow that recently bought the containers I linked in the OP.
He called me to tell me they didn't look the same as my containers and sent me a vid.


As you can see, these are very thin plastic and I don't really like a bit the sound of that lid when he opens it.
Apparently the vendor decided to fuck with me just when I published this tek and they no longer sell the same containers, although the link is still the same as the last box of 500 I bought, with just one little difference in the description that I didn't even notice.

This is the last box I bought:

As you can see it says "heavy duty" and they really were.

Currently it doesn't say "heavy duty" anymore, coz they just aren't. :sad:


I asked my friend a favor, he says he's gonna give them a try and keep some unused plates for 2 months to see if they mold out. Until then we won't know if they're any good. I don't really have high hopes, though.

If someone has bought the containers I linked in the OP, I think it'd be wise to return them unless you wanna take the risk.

I'm sure there must be good containers out there waiting to be found, just take the advice in the OP on how to search for them and be smart about it.

My apologies, I had no way to know.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25150018 - 04/19/18 06:42 AM (3 years, 7 months ago)

Well fuck, I bought 200 of the containers linked in the OP. They do however still have the 'heavy duty' ones available. Picture is exactly the same though so who knows..


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25150023 - 04/19/18 06:51 AM (3 years, 7 months ago)

I hope those are the ones I have, at least it says heavy duty. Let us know, and sorry dude. I'll contact the vendor to ask him what's up.


Edited by Josex (04/19/18 07:00 AM)


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25150025 - 04/19/18 06:57 AM (3 years, 7 months ago)

I just took a look at the items for sale from this vendor. Some containers say "heavy duty" and some don't. So maybe it was just my fault for linking the bad ones, it's the same picture so therein lies the confusion.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25150058 - 04/19/18 07:47 AM (3 years, 7 months ago)

I didn't order the heavy duty ones though, but the ones linked in the OP. I did view other items from the generalstoresltd store, and came across these heavy duty ones. But these are available in more quantities than 200 and 500, so I assumed I had the right ones. I'm interested to hear their response. Might sent them a message myself, but I'm afraid it's too late to exchange my order.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25150059 - 04/19/18 07:48 AM (3 years, 7 months ago)

Just when I run out of shit to invest in...

Josex hooks it up.

So these are like pasty plates on crack eh?

Be nice to skip the condensation/have a better view of my cultures; been trying several things to combat the condensation as it fucks visibility at first.


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Re: Josex' Guide to No-pour Agar. [Re: plurfekt]
    #25150076 - 04/19/18 08:09 AM (3 years, 7 months ago)

@Plur, they're nice but condensation will still be there to haunt you. :evil:

Hey Poison, I hope it's not too late to exchange the order man, def contact the vendor and let us know. What a huge fuck up, sorry again. At least I discovered this relatively soon before more people ordered them.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25150125 - 04/19/18 08:49 AM (3 years, 7 months ago)

I'm considering buying 500 of these. Those are the heavy dutty ones.
I really liked how they looked on your tek and one can even see the PP5 logo on the lids.
The ones on Ebay don't say anything about PP5 neither on product description nor the pictures.
It's hard to find good PP5 cups with PP5 lids.
Joder tio, que embrollo.
I might end up buying urine cups instead since I already know how those work.


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Re: Josex' Guide to No-pour Agar. [Re: Dr. Freeman]
    #25150136 - 04/19/18 09:00 AM (3 years, 7 months ago)

My guess is if it says "heavy duty" those are the ones I have. I have to contact the vendor just to make sure.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25150182 - 04/19/18 09:51 AM (3 years, 7 months ago)

Yeha please do Josex, Its really hard to find deli pots with the rights lids for easy opening in the sab. Tried 4 kinds now.

these look decent.
https://www.ebay.co.uk/itm/100-Round-4oz-Microwave-Plastic-Containers-Lids-Sauce-Baby-Food-Storage-Pots/370795270269?hash=item56551ed07d:g:0LoAAMXQC-tTCivk


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Re: Josex' Guide to No-pour Agar. [Re: Jeebo]
    #25150192 - 04/19/18 09:58 AM (3 years, 7 months ago)

ok Fixed.

These are the ones I use.
It was my fault, I just didn't know the same vendor had similar containers but shittier on sale, and he keeps the same pic for both. :facepalm:


Fuck I feel bad really bad for those of you that bought the ones I linked first, I'm only human, make mistakes, sorry. Fortunately this is Ebay and it won't be a problem to return them.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25150207 - 04/19/18 10:10 AM (3 years, 7 months ago)

Ah okay nice one. I Will get them ordered, cheers. :thumbup:


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Re: Josex' Guide to No-pour Agar. [Re: Jeebo]
    #25150242 - 04/19/18 10:41 AM (3 years, 7 months ago)

@Josex: Well I don't blame you. Making mistakes is human nature, none of us are exempt. The new link you posted is of another vendor though. Are you sure you didn't mean the link Dr. Freeman posted?


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25150252 - 04/19/18 10:51 AM (3 years, 7 months ago)

No man, it's the same vendor I bought from twice, this pic was actually taken from my "purchase history".

Quote:

Josex said:
This is the last box I bought:






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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25150260 - 04/19/18 10:55 AM (3 years, 7 months ago)

Never mind, now I seem to have made a mistake! :blush:


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25150290 - 04/19/18 11:12 AM (3 years, 7 months ago)

Quote:

Poison Drink said:
@Josex: Well I don't blame you. Making mistakes is human nature, none of us are exempt. The new link you posted is of another vendor though. Are you sure you didn't mean the link Dr. Freeman posted?



It's the same product.
The only difference is that Josex's link shows the product in spanish.
I feel really tempted tu buy those.
They look great, but will take some time to get em to my country.
I can get urine cups for about the same price, I might try those in the meantime, the only downside is the coloured lids.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25152325 - 04/20/18 03:48 AM (3 years, 7 months ago)

Quote:

Josex said:
@Plur, they're nice but condensation will still be there to haunt you. :evil:





Just can't win for losing, eh? :lol:

Unfortunately I took a look at buying some of these today and:

Shipping: Does not ship to United States

I'll have to find a vendor state-side.  rip


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Re: Josex' Guide to No-pour Agar. [Re: plurfekt]
    #25152381 - 04/20/18 04:45 AM (3 years, 7 months ago)

Quote:

plurfekt said:
Quote:

Josex said:
@Plur, they're nice but condensation will still be there to haunt you. :evil:





Just can't win for losing, eh? :lol:

Unfortunately I took a look at buying some of these today and:

Shipping: Does not ship to United States

I'll have to find a vendor state-side.  rip




500 Containers

200 Containers


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Re: Josex' Guide to No-pour Agar. [Re: MooseShroom]
    #25152396 - 04/20/18 05:10 AM (3 years, 7 months ago)

Those aren't the ones I use, there was a terrible mistake Mooseshroom, read the posts from yesterday to know why. Shroomery fuckup of the year, sorry for you man.
Plur, the vendor ships some items internationally, others you just have but to ask him and he'll activate international shipping for it.


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Re: Josex' Guide to No-pour Agar. [Re: Josex] * 1
    #25152410 - 04/20/18 05:41 AM (3 years, 7 months ago)

Quote:

Josex said:
Those aren't the ones I use, there was a terrible mistake Mooseshroom, read the posts from yesterday to know why. Shroomery fuckup of the year, sorry for you man.
Plur, the vendor ships some items internationally, others you just have but to ask him and he'll activate international shipping for it.




Appreciated.  The prices on these are very reasonable. 500 containers for 40-45 bucks... Kinda makes me wanna stay with improvised petris over actual petris for a while, even though I bought some of those too - I have no reason to hurry my way to pouring agar beyond better visibility, saving those for when I have a flowhood set up.

Just may inquire about an international shipment, but I don't need 500; maybe he'll sell me like 100. EDIT: (Nvm, at 18$ 200 will do, lmao)

FYI I just checked on my first set of LC's from your biopsy/poke method and they are all looking great, I doubt I'll ever use another method for knocking up liquids. :thumbup::thumbup:


Edited by plurfekt (04/20/18 05:43 AM)


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Re: Josex' Guide to No-pour Agar. [Re: plurfekt]
    #25152413 - 04/20/18 05:44 AM (3 years, 7 months ago)

Great man :thumbup:


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25152635 - 04/20/18 08:53 AM (3 years, 7 months ago)

The UK english site is not the same as the spanish ebay site??
It’s the same parent company.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25152911 - 04/20/18 11:27 AM (3 years, 7 months ago)

Quote:

Josex said:
Those aren't the ones I use, there was a terrible mistake Mooseshroom, read the posts from yesterday to know why. Shroomery fuckup of the year, sorry for you man.
Plur, the vendor ships some items internationally, others you just have but to ask him and he'll activate international shipping for it.




What's so different about them?


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Re: Josex' Guide to No-pour Agar. [Re: MooseShroom]
    #25152942 - 04/20/18 11:35 AM (3 years, 7 months ago)

Can you post a pic of one your containers? An empty one, wanna see the sides of the cup.

I've been talking to the customers service of generalstores, bitch doesn't even know what she's talking about, says they only have listed the containers with those weird bands on the cup (the thin plastic ones), which are the same you can see in the pic they have.
But I bought the heavy duty ones from them twice, no marks on the cup, thick plastic and the pic they had for those containers I bought was the same one with the bands.

I've sent them another pm with pics to show them. I'll tell yall what they say tomorrow.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25152995 - 04/20/18 11:56 AM (3 years, 7 months ago)

Very confusing stuff, she even asked me if I was sure, coz they only have those thin containers with the bands and that they can't sell anything that doesn't show in the pic.
Thankfully I have pics of my purchase history and pics of my actual containers to show her.
Bought from them twice, a year and a half between both purchases.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25153133 - 04/20/18 01:21 PM (3 years, 7 months ago)



Guess they are slightly different from the ones in your guide.
Looks like the exact same containers as the video listed a few posts back


Edited by MooseShroom (04/20/18 01:41 PM)


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Re: Josex' Guide to No-pour Agar. [Re: MooseShroom]
    #25153196 - 04/20/18 02:01 PM (3 years, 7 months ago)

Any issues so far with them? Also, can you do me the favor of keeping some unused plates for more than a month? Wanna see if they mold out without opening them. Thanks Moose.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25153803 - 04/20/18 06:33 PM (3 years, 7 months ago)

@MooseShroom: I'm interested in your experiences with these containers as well. I ordered the ones originally linked in the OP, so chances are high I'm going to receive the same ones you have.


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25153987 - 04/20/18 08:24 PM (3 years, 7 months ago)

I've had good experiences with them so far. The hardest part is getting them open smoothly. I've gotten down a pretty good method of doing like a quick swirl around instead of cracking in a spot here and there. The fluid motion of it gets the lid off easily whereas cracking them open pulls open one side and consequently closes the other.

@Josex I've left these plates out for a month and haven't had any contamination issues that I can see. If there's contamination on them, it's extremely sneaky.


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Re: Josex' Guide to No-pour Agar. [Re: MooseShroom]
    #25154548 - 04/21/18 04:12 AM (3 years, 7 months ago)

Thank god man (sigh of relief). Being thinner plastic maybe visibility is even better than my containers.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25154598 - 04/21/18 05:45 AM (3 years, 7 months ago)

I was thinking if I would return the shipment, I would most likely only get a refund for the price of the containers and not their shipping costs. I would also have to pay shipping costs myself to send them back. Considering I only bought 200 of them, the total amount I would have lost by returning them would be almost as much as the original order. So I might as well keep them and give them a go! The whole order was rather cheap anyhow, so no big loss. I'm still interested to hear how matters stand though, for possible future purchases.


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25155275 - 04/21/18 02:24 PM (3 years, 7 months ago)

The visibility on them is pretty great. I haven't had any issues with them yet but have no clue how long they'll hold up. Been through 4 pressure cooker cycles so far on some of them and they still make an audible crack when I put the lids on. I'll probably just stick with these since they work for me even if they aren't the perfect container. :shrug:


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Re: Josex' Guide to No-pour Agar. [Re: MooseShroom]
    #25155303 - 04/21/18 02:37 PM (3 years, 7 months ago)

@MooseShroom: Thanks for the reassurance!


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25162073 - 04/24/18 12:52 PM (3 years, 7 months ago)

Ok dudes, after being pretty much ignored by generalstores, finally I could get them to listen.
Apparently they don't sell the containers I use anymore, only the ones that I linked in the OP, which seem decent enough according to some members here that bought them, so I'll keep the link in the OP for those who may have trouble finding good containers.

I'd like to encourage people to look for containers on the internet and try them out so you can link them here for others to benefit if they happen to be good.

If you want to understand where all this confusion about the containers is coming from, take a look at the pics, it's the conversation I had with the bitch in charge of customer's service.





It was their fault, they had the exact same pic for the containers I use and the containers I linked, how would I know? :shrug:
From my purchase history:



The current pic they have (the same) which are not containers I use:



Edited by Josex (04/24/18 01:02 PM)


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25162224 - 04/24/18 02:05 PM (3 years, 7 months ago)

Appreciate your efforts Josex, I'm keeping my eyes peeled for new PP5 containers to test online and off. :mushroom2:


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Re: Josex' Guide to No-pour Agar. [Re: plurfekt]
    #25162253 - 04/24/18 02:15 PM (3 years, 7 months ago)

:super:


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25162414 - 04/24/18 03:35 PM (3 years, 7 months ago)

you should of added 'a bunch of pissed off drug people will be returning product'. :crankey:

thats some mighty fine lookin out josex. that boy do good.:awesomenod:


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Re: Josex' Guide to No-pour Agar. [Re: mushboy]
    #25162423 - 04/24/18 03:40 PM (3 years, 7 months ago)

:lolsy:


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25162476 - 04/24/18 03:58 PM (3 years, 7 months ago)

I've been looking around for different containers and I'm having an easy time finding the cups but a hard time finding matching lids haha. I'll update this if I find anything that looks reassuring.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25162498 - 04/24/18 04:05 PM (3 years, 7 months ago)

Nice tek dude =) Really nice pointers, I just jumped straight to pouring petri's in a SAB (still have not inoculated yet, waiting on spores still :mad:). Also I found this, perhaps these will do the job, and free shipping internationally =)

Anyway I might try this TEK once in the future, if so; perhaps I will upload the results =)

Peace and love :happyheart:


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Re: Josex' Guide to No-pour Agar. [Re: iux]
    #25166229 - 04/26/18 08:36 AM (3 years, 7 months ago)

My plastic containers arrived in the mail today. As mentioned before, they're rather tricky to open smoothly. Nonetheless I'm still going to give them a go, even though it will probably require some practice to get used to. I have found an alternative method to make the little holes by using a hole punch pliers. that way I can make a neat little hole every time.




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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25166263 - 04/26/18 08:52 AM (3 years, 7 months ago)

Visibility might even be better than my containers because the plastic is thinner, but yeah such a bummer that they won't open smoothly.

Nice nifty thing you got there to make them holes! :derfase:


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25166355 - 04/26/18 10:04 AM (3 years, 7 months ago)

Yeah good use of the tool indeed :tongue2:


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Re: Josex' Guide to No-pour Agar. [Re: iux]
    #25166776 - 04/26/18 02:42 PM (3 years, 7 months ago)

It's normally used to make extra holes in a belt. You need to exert some force to get it through, but it works fine with this type and thickness of plastic. I was afraid it might shatter the container, but I guess PP doesn't have this property.

@Josex: How do you clean out these containers after use? I didn't know you could reuse MP tape filters until I read this TEK. I use these filters for my grain jars too, but I would always replace the tape, which is a bit of a hassle. I would usually scrub them clean in water and put them in the dish washer.


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25166779 - 04/26/18 02:45 PM (3 years, 7 months ago)

No need to wash them with water, that's a hell of a hassle.

This is in the OP, it's what I do:
Quote:

Josex said:
CLEANING THE PLATES AFTER USE

Due to the fact that these containers are very rigid plastic, they don't allow you to squeeze the agar puck out like glad mini rounds. Instead, I take my scalpel and detach the puck from the very edge using the tip of the blade. It comes out easy and quick and weird enough, I find it to be a strangely satisfying task. :lol:

Then I wipe down the cup and the lid with a dry paper towel, snap the lid on and store the empty plates like that for later use.





If you use MP tape for grains jars too I'd def replace them everytime in that case.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25166821 - 04/26/18 03:14 PM (3 years, 7 months ago)

Alright I seem to have missed that, thanks! Why would it make a difference between grains and agar though? Also, if you had a contamination, could you still reuse the MP tape without any problems?


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25166861 - 04/26/18 03:40 PM (3 years, 7 months ago)

Quote:

Poison Drink said:
Why would it make a difference between grains and agar though?



MP tape by itself as a filter for grain jars gets pretty beaten up during the PC cycle alone. There is a lot of gas exchange going on through the filter while you vent the PC and the tape gets brownish with grain particles.

Also, the tape will suffer during colonization with all that biological activity taking place, not the case with agar plates.

MP tape can be used for grain jars but it's less than ideal, can't you source polyfil instead? That's a very good filter and dirt cheap, and if you stuff it right and tight it will also be good for several PC cycles.

Quote:

Poison Drink said:
Also, if you had a contamination, could you still reuse the MP tape without any problems?



You mean containers for agar? Of course, you'll have to pc them again anyway, everything on the filter gets killed.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25166946 - 04/26/18 04:16 PM (3 years, 7 months ago)

Maybe I should look into using SFD's. Here in the EU I never really came across polyfill. It might be sold under another name though. I recently started with this hobby again so I should do some reading up. But anyway, I won't continue going off-topic in your thread.


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25166951 - 04/26/18 04:18 PM (3 years, 7 months ago)

Quote:

Poison Drink said:
It might be sold under another name though.




Polyester stuffing. I can't source it locally myself. Ebay is a great thang, though. :wink:


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25166971 - 04/26/18 04:31 PM (3 years, 7 months ago)

Quote:

Poison Drink said:
Maybe I should look into using SFD's. Here in the EU I never really came across polyfill. It might be sold under another name though. I recently started with this hobby again so I should do some reading up. But anyway, I won't continue going off-topic in your thread.



Polyfill can be found inside hypoallergenic pillows and stuffed teddy bears.


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Re: Josex' Guide to No-pour Agar. [Re: Dr. Freeman]
    #25166986 - 04/26/18 04:34 PM (3 years, 7 months ago)

True that, but I've found not all poly is the same quality, I ripped apart quite some pillows in the past and the poly was shitty indeed.
These bags they sell on Ebay for toy stuffing are usually pretty good, it will go a long way too.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25172124 - 04/28/18 07:11 PM (3 years, 7 months ago)

Josex you're awesome man, the amount of time you've put into helping others.

Top bloke, with the receipe With 16,5 grams of agar, 16,5 grams of LME and 1100 ml of water I can make 120 plates.

With that there in place looks like the receipe i used was waay off (got it off youtube)

I just want to make about 8 plates, Going by that... 16.5 divded by 120 = 0.1375gram per plate, So if i only want to knock up 8 plates, would 1.1grams of each be enough? This is very confusing to find the right ratio/math to work on when I've read so much different info.

I could of sworn i used 5grams of each i used Liquid malt aswell with 500ml of water or 250ml i forget, read a few teks etc so much miss-infomation out there it's not funny. My agar did not set properly i think due to i didn't disolve it with heat just stirred, loaded & PC'ed. Looks like i've still got growth though.


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Edited by mindblowing (04/28/18 07:13 PM)


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Re: Josex' Guide to No-pour Agar. [Re: mindblowing]
    #25173362 - 04/29/18 01:29 PM (3 years, 7 months ago)

First thing you need to know is that when we refer to nute strenght or agar strength in percentages we're actually talking grams per 100ml of water. So for instance, 2% agar strength means 2 grams per 100ml of water.
I'd recommend you do the standard at first (2% nutes and 2% agar), because not all agar are the same and the strength may vary from brand to brand.

Usually, with 120ml of water you can make from 8 to 12 plates if you're using 100ml containers, it varies depending on how wide the bottom of the container is. Best way to know for sure how many ml of water per container you need is taking a syringe and squirting as much water as you'd do if you were pouring actual agar, then multiply those ml you used by the number of containers you're going to be using.

For example, imagine you're gonna make 14 plates. Let's say each plate will receive 10ml of water (140ml in total), plus 20ml more because you don't wanna run short by accident, so 160ml in total.

The following is the equation you always need to do, so for this example we're gonna make agar at 2% nute strength and 2% agar strength, using 160ml of water:

1. Multiply total amount of water by nute strength:
160*2 = 320
2. Divide the result by 100:
320/100 = 3,2.
3. The result is the number of grams of nutrients you need to make agar at 2% nute strength for 160ml of water, so in the example you'd need 3,2 grams of nutes.

The result in the example is also going to be 3,2 grams of agar in this case, because both nutes and agar are 2% strength. So you'd need 160ml of water, 3,2 grams of agar and 3,2 grams of nutes. But you'd need to do the equation again if you wanted to do 1,5% agar strength.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25174120 - 04/29/18 08:18 PM (3 years, 7 months ago)

How long would you normally wait after PC'ing to unload? I noticed some of my containers were dented after taking them out. I would let the PC release its pressure naturally, and after that I would give it another five minutes before unloading the PC. Maybe this is too early, because the containers and agar are still very hot. The little hole with MP filter might not be able to keep up equalizing the pressure due to large temperature differences? Also, if I open up the PC I hear some crackling noises. Anyway I see there will be a learning curve using these containers! Nonetheless I PC'd two batches of 15 containers two days ago, and none seem to be having any contamination as of yet.


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25174138 - 04/29/18 08:26 PM (3 years, 7 months ago)

My holes are smaller than yours and I don't have those problems. I open the PC as soon as the pressure drops, no weird sounds or dented containers. Mine are thick plastic but I don't see why you'd have those issues with thinner plastic. You're using MP tape (3M brand) right? It allows plenty of GE to prevent the containers from warping during the PC cycle and also when you take them out hot, so the temp difference shouldn't be a problem.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25174171 - 04/29/18 08:43 PM (3 years, 7 months ago)

Yes, two layers of micropore 3M brand. I did notice those dented containers were all next to each other on the table top, so they must have come from the same layer in the PC. I don't recollect if it were the top or bottom ones though. I will keep an eye on it the next time. I did however skip the aluminum foil step, since I ran out of it. My PC only holds 15 containers so I didn't mind to carefully dry them off this time. Could this have caused it?


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25174193 - 04/29/18 08:55 PM (3 years, 7 months ago)

If you don't use the layers of foil and instead stack them normally in the PC they'll form a vacuum between the lid of the container below and the bottom of the container above, but idk if that could be the cause for the dented containers, I did that a couple times with mine and didn't have any issues. Maybe some containers got dents from contact with the lid of the PC? Def use foil next time.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25174251 - 04/29/18 09:20 PM (3 years, 7 months ago)

They came off each other smoothly, so I wouldn't say that's the problem. The upper containers weren't touching the lid, but they might have been touching the side of the PC. Next time I will try to keep them off the side and see how it goes. Anyway, I'll just need to do some trail and error with these! Thanks for your help.


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Re: Josex' Guide to No-pour Agar. [Re: Poison Drink]
    #25180077 - 05/02/18 04:19 PM (3 years, 7 months ago)

Josex man,

Got any idea why my plates didn't set?

I took in all that infomation about making up agar i understand that shit, Cheers dude. So i just whipped up 250ML so 5g, i put it on the stove disvoled it for 2 minutes or so, Poured it into my containers and it set fine was pretty firm in 30 seconds or so, seems to have no problems setting..

Alright so i put them in my PC 2 layers of micropore tape, Paper town over that and wrapped in alfoil and loaded in my pc. As i read it does not matter bringing the temp up slowly.. So i put it on full heat got the pressure to 15PSI and PC'ed for 45 minutes. Now i finished the cycle at 11PM set an alarm for 3hours but i didnt fucking wake up... So it's been 7hrs untill i got to unload the containers i know that's to long but they should of still set?

Okay so what the fuck happened plates came out with moisture in them and not set one bit, there fucked. It defintly seems like the PC has over boiled them or something, 45 minutes to long? Aswell as getting it to 15psi etc that's close to an hour pc'ing is that to long? You sure we should not bring the PC up slowly? I'm just thinking last batch of agar i did i brang it up very slowly and it didn't seem to fuck the agar like last time. The agar is pretty dam runny not set at all, So at what stage it seems like the PC has got the agar to hot and fucked it, idk.. I'm pretty bummed about this, this fucking sucks. It's a long cycle to have these come up fucked...... :frown:(((

Thanks man


--------------------


Edited by mindblowing (05/02/18 04:22 PM)


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Re: Josex' Guide to No-pour Agar. [Re: mindblowing]
    #25180109 - 05/02/18 04:38 PM (3 years, 7 months ago)

Your question is a thread jack and should be answered where you posted it the first time to the tcs or start your own thread.


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Re: Josex' Guide to No-pour Agar. [Re: mindblowing]
    #25180244 - 05/02/18 05:52 PM (3 years, 7 months ago)

Quote:

mindblowing said:
Josex man,

Got any idea why my plates didn't set?

I took in all that infomation about making up agar i understand that shit, Cheers dude. So i just whipped up 250ML so 5g, i put it on the stove disvoled it for 2 minutes or so, Poured it into my containers and it set fine was pretty firm in 30 seconds or so, seems to have no problems setting..

Alright so i put them in my PC 2 layers of micropore tape, Paper town over that and wrapped in alfoil and loaded in my pc. As i read it does not matter bringing the temp up slowly.. So i put it on full heat got the pressure to 15PSI and PC'ed for 45 minutes. Now i finished the cycle at 11PM set an alarm for 3hours but i didnt fucking wake up... So it's been 7hrs untill i got to unload the containers i know that's to long but they should of still set?

Okay so what the fuck happened plates came out with moisture in them and not set one bit, there fucked. It defintly seems like the PC has over boiled them or something, 45 minutes to long? Aswell as getting it to 15psi etc that's close to an hour pc'ing is that to long? You sure we should not bring the PC up slowly? I'm just thinking last batch of agar i did i brang it up very slowly and it didn't seem to fuck the agar like last time. The agar is pretty dam runny not set at all, So at what stage it seems like the PC has got the agar to hot and fucked it, idk.. I'm pretty bummed about this, this fucking sucks. It's a long cycle to have these come up fucked...... :frown:(((

Thanks man



As a great poet once said "fucking the follow tek". :rockon:

Seriously man, I've no clue what happened there, the issue can be something you didn't even mention, that's my guess. Bringing the pc up to pressure slow or quick doesn't cause what happened to you. Did you take off the rocker before letting the pc drop to zero? A sudden pressure drop can cause boiling over. Maybe you didn't raise the plates above the water or your conainters have short sides or the lids are shitty...

Also I don't know how you managed to get the mix ready in just 2 min in the pot, takes longer than that for me, but I don't think that was the issue either.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25180251 - 05/02/18 05:57 PM (3 years, 7 months ago)

the only reason my agar never set is because my scale wwas broken and i used the wrong amount.

translation - you fucked up:shrug:


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Re: Josex' Guide to No-pour Agar. [Re: mushboy]
    #25180255 - 05/02/18 05:59 PM (3 years, 7 months ago)

That's a good one.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25180270 - 05/02/18 06:07 PM (3 years, 7 months ago)

Awesome write-up!! Thanks for your time.

I am confused whats going on in the video. You're cutting a small piece of colonized agar, dipping it into a clean dish, removing the piece of agar, and doing what with it? Sorry if this was explained in the org tutorial. But I didnt see anything.

Could you explain?


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Re: Josex' Guide to No-pour Agar. [Re: sMileHighCity]
    #25180280 - 05/02/18 06:14 PM (3 years, 7 months ago)

That's a practice outside the SAB open air, just to illustrate how to work with containers upside-down and how to scoop up the wedge so it doesn't get stuck in the blade and also to bring home to beginners the importance of doing practices like that to get familiar with the scalpel. In the SAB you want to move more carefully than in the vid though, I just didn't feel like recording it in the SAB that day, too lazy.


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Re: Josex' Guide to No-pour Agar. [Re: mushboy]
    #25180288 - 05/02/18 06:18 PM (3 years, 7 months ago)

Quote:

mushboy said:
the only reason my agar never set is because my scale wwas broken and i used the wrong amount.

translation - you fucked up:shrug:




No like i said, agar set fine pre-pcing.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25180293 - 05/02/18 06:22 PM (3 years, 7 months ago)

Quote:

Josex said:
Quote:

mindblowing said:
Josex man,

Got any idea why my plates didn't set?

I took in all that infomation about making up agar i understand that shit, Cheers dude. So i just whipped up 250ML so 5g, i put it on the stove disvoled it for 2 minutes or so, Poured it into my containers and it set fine was pretty firm in 30 seconds or so, seems to have no problems setting..

Alright so i put them in my PC 2 layers of micropore tape, Paper town over that and wrapped in alfoil and loaded in my pc. As i read it does not matter bringing the temp up slowly.. So i put it on full heat got the pressure to 15PSI and PC'ed for 45 minutes. Now i finished the cycle at 11PM set an alarm for 3hours but i didnt fucking wake up... So it's been 7hrs untill i got to unload the containers i know that's to long but they should of still set?

Okay so what the fuck happened plates came out with moisture in them and not set one bit, there fucked. It defintly seems like the PC has over boiled them or something, 45 minutes to long? Aswell as getting it to 15psi etc that's close to an hour pc'ing is that to long? You sure we should not bring the PC up slowly? I'm just thinking last batch of agar i did i brang it up very slowly and it didn't seem to fuck the agar like last time. The agar is pretty dam runny not set at all, So at what stage it seems like the PC has got the agar to hot and fucked it, idk.. I'm pretty bummed about this, this fucking sucks. It's a long cycle to have these come up fucked...... :frown:(((

Thanks man



As a great poet once said "fucking the follow tek". :rockon:

Seriously man, I've no clue what happened there, the issue can be something you didn't even mention, that's my guess. Bringing the pc up to pressure slow or quick doesn't cause what happened to you. Did you take off the rocker before letting the pc drop to zero? A sudden pressure drop can cause boiling over. Maybe you didn't raise the plates above the water or your conainters have short sides or the lids are shitty...

Also I don't know how you managed to get the mix ready in just 2 min in the pot, takes longer than that for me, but I don't think that was the issue either.




Thanks anyway man for you're advice. Nah i kept the weight on i let it cool down naturally, plates above water spaced out with a alfroil made spacer aswell.

I followed the tek spot on besides like you just said using retarted containers, short side would that really cause the boilover & fucked the agar tho?


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Re: Josex' Guide to No-pour Agar. [Re: mindblowing]
    #25180301 - 05/02/18 06:25 PM (3 years, 7 months ago)

Any pics of your containers?


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25180705 - 05/02/18 10:35 PM (3 years, 7 months ago)

It's quite obvious I thought, you left the agar too long and it set unlevel in the cold PC.

Happens to me all the time when I get too intoxicated and forget about the plates, its a real bitch.


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Re: Josex' Guide to No-pour Agar. [Re: Jeebo] * 2
    #25180774 - 05/02/18 11:20 PM (3 years, 7 months ago)

Quote:

Jeebo said:
It's quite obvious I thought, you left the agar too long and it set unlevel in the cold PC.

Happens to me all the time when I get too intoxicated and forget about the plates, its a real bitch.




Hmmm, It could be that and/or the containers..

The agar is not really set how it should be like it's a bit runny.. so it's not really set "unlevel" I think the containers are a bitch WAY to much moisture is getting into them effecting the agar to re-set properly is what i'm more thinking.


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Re: Josex' Guide to No-pour Agar. [Re: mindblowing]
    #25180979 - 05/03/18 02:35 AM (3 years, 7 months ago)

Thanks for this! Been debating experimenting with agar. This helps encourage me to stop procrastinating


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Re: Josex' Guide to No-pour Agar. [Re: mindblowing]
    #25181640 - 05/03/18 12:55 PM (3 years, 7 months ago)

Quote:

mindblowing said:
Quote:

Jeebo said:
It's quite obvious I thought, you left the agar too long and it set unlevel in the cold PC.

Happens to me all the time when I get too intoxicated and forget about the plates, its a real bitch.




Hmmm, It could be that and/or the containers..

The agar is not really set how it should be like it's a bit runny.. so it's not really set "unlevel" I think the containers are a bitch WAY to much moisture is getting into them effecting the agar to re-set properly is what i'm more thinking.



Yeah you should just buy other cotainers, the ones you posted earlier in this thread look horrible for agar, pretty sure all your problems come from that.
Quote:

mindblowing said:

I got some sqaureish containers, I left them overnight, etc and my filter (ontop) is asboultely drenched, It was 2 peices of micorpore and that is stuffed aslong with the paper towl that was stuck to it ontop...

Should i bother trying these containers? Just placing another peice of tape ontop, Let them dry out for a few hours in my sab & try them?

I carefully 1 by 1 opened them and poured out the excess water, was only a few drops some a good 10 or so, My agar looks like it didn't set as well as it could of aswell. I know what mistakes i have done now thanks to this writeup!!!

Honestly, Should i try inccoulate these containers? I am tempted to move onto pouring i think i'd enjoy it better




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Re: Josex' Guide to No-pour Agar. [Re: Josex] * 1
    #25182308 - 05/03/18 07:00 PM (3 years, 7 months ago)

Yeah like i mentioned im not using containers OP got

Anyone who's reading this make sure you take the time to get the right containers

https://files.shroomery.org/files/18-17/496481457-20180429_110654.jpg

Get the ones op linked im going.to buy very similar locally


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Re: Josex' Guide to No-pour Agar. [Re: mindblowing]
    #25186346 - 05/05/18 07:36 PM (3 years, 7 months ago)

How many time can I keep the containers pc'ed in the tote? I am going to do it tomorrow since i get it today the containers (200) but im going to use only like 20-40/week. Will they last for like 2-3 months sterile?


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Re: Josex' Guide to No-pour Agar. [Re: roses4lg] * 1
    #25186569 - 05/05/18 10:10 PM (3 years, 7 months ago)

I've kept plates for more than 2 months and used them without issues. They didn't even lose all the condensation, let alone dry out. A small hole and several layers of tape and you're good. :thumbup:
If the containers you're using are decent enough they shouldn't contaminate in 2 months.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25187855 - 05/06/18 03:33 PM (3 years, 7 months ago)

Cool! Thanks mate <3


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Re: Josex' Guide to No-pour Agar. [Re: roses4lg]
    #25188038 - 05/06/18 05:39 PM (3 years, 7 months ago)

By the way I did some agar but It's too late here where I live and I'm gonna pc the leftovers containers tomorrow since I did too much and don't fit in the pc. Should I do them again and toss them or its safe to pc'ed tomorrow?


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Re: Josex' Guide to No-pour Agar. [Re: roses4lg]
    #25188093 - 05/06/18 06:20 PM (3 years, 7 months ago)

Yea you can pc them tomorrow, you'll know when it's too late to pc them when they mold out. One day is ok, though.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25188795 - 05/07/18 02:25 AM (3 years, 6 months ago)

Yeah! Btw Im doing a journal with your Tek
https://www.shroomery.org/forums/showflat.php/Number/25188202

:laugh:


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Re: Josex' Guide to No-pour Agar. [Re: roses4lg]
    #25189064 - 05/07/18 09:21 AM (3 years, 6 months ago)

Wish you luck with those containers, with deli containers as long as the close fairly tight you're good.

Read your journal and saw what you wanted to do with those larger containers and the SHIP's. There's a better way to make LC imo, but instead you use glass containers and inject water into the culture instead of nutes, take a look.
https://www.shroomery.org/forums/showflat.php/Number/23740857#23740857


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25189284 - 05/07/18 11:40 AM (3 years, 6 months ago)

Oh! I didn't know that, so I can save the nutes and use only sterile water.

Yeah! Thanks <3


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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: Guía de Josex para No-verter Agar. </font></font> [Re: roses4lg]
    #25224883 - 05/24/18 09:36 AM (3 years, 6 months ago)

@josex those jars are what here in Spain use Chinese restaurants for sweet and sour sauce?


Edited by turbo (05/24/18 10:54 AM)


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Re: Josex' Guide to No-pour Agar. [Re: roses4lg]
    #25224976 - 05/24/18 10:18 AM (3 years, 6 months ago)

You can say it's that type of container. The ones you can find in restaurants for takeaway food are usually very bad quality though and non-suitable for agar work.

If you can, search around on Ebay. Finding the right containers takes patience and some cunning, but there are good containers out there waiting to be found.

I'm afraid the ones I use are no longer for sale, otherwise I'd give you the link. The OP tells you how to look for them and the type of container you want. Good luck. :thumbup:


Edited by Josex (05/24/18 10:43 AM)


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25281935 - 06/20/18 10:28 PM (3 years, 5 months ago)

I'm considering switching to no-pour using these jars but am hesitant after just learning and having success with the pour method. For pour, I like the visibility and ease of movement/stacking in the SAB, but the cost of plastic petris and the need to always buy is dissuading me from continuing it.
My question, how much is visibility compromised in your jars compared to petri? Anything you can say to persuade me to switch?


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Re: Josex' Guide to No-pour Agar. [Re: hermanium]
    #25282012 - 06/20/18 10:56 PM (3 years, 5 months ago)

I'm well aware the OP is such a TL;DR, just a bad habit I have, but if you have the time to read it all I'm pretty sure it'll persuade you.

I wouldn't switch to petris even if I had them for free, no kidding. The only thing they have to offer imo is better visibility and maybe a bit more size. The thought of having to pour them, wrap them up and buy new boxes is a huge deterrent for me.

You can also stack this type of containers,. You can make a fuck ton of them one day and they'll be good for up to 2 months or more, so you'll always have blank plates handy. You don't have to wrap them before pc'ing and the MP tape will last many many cycles so no need to change it, just add another layer on top of the old ones when these deteriorate. They're super fast and easy to clean, very ergonomic and easy to handle. I could go on and on but it's all in the OP.

The cons... it's not easy to find appropriate containers, visibility can't compare with petris (but tbh is good enough to distinguish growth and contams and even take decent pics sometimes), although the critical moment when you really need to see the culture is when you open the lid to take a wedge. They take more space than petris too, but very little and you can stack them really tall.


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Invisiblehermanium

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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25282089 - 06/20/18 11:31 PM (3 years, 5 months ago)

I appreciate your response, I had read parts of the OP looking for that comparison but I'll reread it entirely now.


Edited by hermanium (06/20/18 11:35 PM)


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Re: Josex' Guide to No-pour Agar. [Re: hermanium]
    #25282102 - 06/20/18 11:37 PM (3 years, 5 months ago)

No, I wouldn't. It was the exact same link, same vendor and the exact same pic as the containers I use. So I shared that link in the OP with total confidence when I published it, only to discover days later when some people bought them that they weren't the same containers I use.

According to the people who bought them, those are not that bad. The plastic is much thinner than mine and the lids are a bitch to open apparenty, but you can work with them.

I'd look for better ones if I was you, though.

Edit: Mental note... always quote people before they decide to do a ninja edit. :rofl:
What I said above won't make sense now lol


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Invisiblehermanium

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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25282118 - 06/20/18 11:43 PM (3 years, 5 months ago)

Sorry I removed that link after I realized it didn't ship to the U.s. Ugh, it's bedtime.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25369162 - 08/06/18 12:45 AM (3 years, 4 months ago)

thank you so much for this tek great info bookmarked for sure my friend

:sporedrop:


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Re: Josex' Guide to No-pour Agar. [Re: Fungeyes]
    #25431443 - 09/02/18 09:46 PM (3 years, 3 months ago)

Has anyone every used a syringe filters  on no pour plates instead of micropore. I have 100s of them but no micro pore atm wondering if they would work instead


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Re: Josex' Guide to No-pour Agar. [Re: bakenast]
    #25486950 - 09/25/18 01:17 AM (3 years, 2 months ago)

1st attempt in progress


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Re: Josex' Guide to No-pour Agar. [Re: motifaded] * 1
    #25489977 - 09/26/18 09:57 AM (3 years, 2 months ago)

Hola muchachos!
Just checking in to ask the guys who ended up ordering the "wrong ones",
now with some time passed, what's your conclusion about them?
Sucks being in europe when it comes to mushroom growing equipment...


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Re: Josex' Guide to No-pour Agar. [Re: OericOss]
    #25490587 - 09/26/18 03:32 PM (3 years, 2 months ago)

I'm still using the "wrong" plates with great success. I've become accustomed to using the lids at this point. I've used a plate that was 2 months old successfully so they work just fine. Haven't noticed any degradation through pressure cooker cycles yet either. Not the perfect container, but I still quite like them. I can fit exactly 60 of them in my 23q Presto, which works perfectly for my needs.

Here's a current culture I have going in one of them:


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #25660788 - 12/06/18 03:52 AM (3 years, 3 days ago)

Nice thread for sure


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OfflineEbilPhish
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #26051754 - 06/14/19 09:07 AM (2 years, 5 months ago)

Whats the problem with the ones with 'hinged' lids?

Is it just that they are hard to open? Or is their some contamination due to crappy lid.

I have a bunch around here from getting take-away with dipping sauce. The hinges could be cut off if not being able to remove the lid is the issue. I did try one upside-down with water and found it leaked though.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #26054397 - 06/15/19 07:20 PM (2 years, 5 months ago)

:takingnotes:


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Re: Josex' Guide to No-pour Agar. [Re: lilwoman] * 1
    #26066320 - 06/21/19 09:33 PM (2 years, 5 months ago)

Did anyone find a source in the U.S.?


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Re: Josex' Guide to No-pour Agar. [Re: HemiSync]
    #26137455 - 08/15/19 07:17 PM (2 years, 3 months ago)

Thoughts on a no-pour way to make slants for storage of cultures?


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Offlinecrosstree
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Re: Josex' Guide to No-pour Agar. [Re: HemiSync]
    #26199740 - 09/20/19 06:54 PM (2 years, 2 months ago)

I found and just ordered some of these to try out. Free (and long) shipping from China to US, will try to remember to update on quality. ebay.com/itm/142706597557

update:
These worked just fine for the tek but 10 of the 50 ordered came broken. Has been a great way to start agar, thanks Josex:thumbup:!


Edited by crosstree (10/13/19 11:09 PM)


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Re: Josex' Guide to No-pour Agar. [Re: crosstree]
    #26206850 - 09/24/19 05:23 PM (2 years, 2 months ago)

Hi! I hope bumping this thread isnt against the law :laugh:

I just wanted to ask you what would your advice be regarding these two types of containers. They are both about 70mm in diameter and 30-50mm in height.

Which would you choose?



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Re: Josex' Guide to No-pour Agar. [Re: Sankhara]
    #26209340 - 09/25/19 08:43 PM (2 years, 2 months ago)

Neither of those look like they will stand a pressure cooker to me. Is there a plastic type written?


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Re: Josex' Guide to No-pour Agar. [Re: crosstree]
    #26209442 - 09/25/19 09:32 PM (2 years, 2 months ago)

Yes! They are plastic PP5 they should withstand pc in theory...

Its the only shit i can get around here unfortunately.

Would you recommend any of those?


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Re: Josex' Guide to No-pour Agar. [Re: FeedYourMind]
    #26216194 - 09/28/19 03:40 PM (2 years, 2 months ago)

dam, good tek


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Re: Josex' Guide to No-pour Agar. [Re: mi.ro.ku]
    #26515047 - 03/03/20 07:20 PM (1 year, 9 months ago)

Started out with 8oz, they're a bit big but are great for growing out a culture to make a slurry. Bought some of the wee 4oz ones and they rock! Condensation clears up quicker, tighter seal on the lids, and are great transfer plates.

Cheers Josex :rockon:



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Re: Josex' Guide to No-pour Agar. [Re: FeedYourMind]
    #26782466 - 06/25/20 06:13 PM (1 year, 5 months ago)

What is best way to clean out the discard containers? Does dishwasher screw the micro pore tape?


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InvisibleJosex
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Re: Josex' Guide to No-pour Agar. [Re: Fuglytwatapuss]
    #26800681 - 07/03/20 05:54 AM (1 year, 5 months ago)

Quote:

Fuglytwatapuss said:
What is best way to clean out the discard containers? Does dishwasher screw the micro pore tape?




That's in the tek too. Scroll down til almost the end of the OP and you'll se it. That's what I do. There's no need for plain soap and water, let alone the dishwasher.
You won't really need to wash them, ever. Unless of course you have some disgusting looking contaminated plates or in those cases where you let your plates dry the fuck up.


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Offlinehardinthekitchen
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #26804399 - 07/05/20 12:57 AM (1 year, 5 months ago)

Amazing write up. Thank you :smile: I forgot to put the last layer of tinfoil on! are those ones toast??


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #26808710 - 07/07/20 12:12 PM (1 year, 4 months ago)

This is a great write-up.  Thanks for all of the time and patience to do it.  I love the bullshit list, because condensation is driving me nuts and I've been worried about it.

I found these in the US, but the price and quantities are a little wonky.  https://www.berlinpackaging.com/29104-4-oz-natural-pp-plastic-round-containers-snap-lock-lid/?promo=shopping&gclid=Cj0KCQjwupD4BRD4ARIsABJMmZ-_Uniq_Up68iIV-6rjU_J4QouDgKP8obXMOMYuVOh842bS-KtVKDkaAuQSEALw_wcB

Anyone else have a solid lead on these in the US?  Amazon has Vito's famous deli cups but I think the lids might not be PP5.


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Re: Josex' Guide to No-pour Agar. [Re: maxheadroom]
    #26810284 - 07/08/20 06:22 AM (1 year, 4 months ago)

Thanks man. Be careful though, I clicked on that link and it says in the description that the lids are LDPE, not PP5. They'd melt on you.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #26813301 - 07/09/20 06:52 PM (1 year, 4 months ago)

Is there any new source for some of these plates? There is some on amazon that are very similar in 4oz and 2oz. Would a 2oz container work? It's 1.1 inches tall.


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Re: Josex' Guide to No-pour Agar. [Re: Zeppelinthecat]
    #26814500 - 07/10/20 09:48 AM (1 year, 4 months ago)

That'd be too small. Try eBay too.


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Thank you ! [Re: Josex]
    #26848912 - 07/28/20 12:01 AM (1 year, 4 months ago)

Thank you, Josex, for this! Very informative and just when I needed it. 😁


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Re: Thank you ! [Re: CyberRacoon]
    #26866045 - 08/06/20 11:10 AM (1 year, 4 months ago)

I forgot about my plates in the PC and took them out to cool a little late and caused tiny bubbles in the agar. My question is can I resterilize them to get rid of the bubbles?


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Re: Josex' Guide to No-pour Agar. [Re: maxheadroom]
    #26891889 - 08/21/20 04:25 PM (1 year, 3 months ago)

would these be okay? PP5 on the lid and cup, but the bottoms are slightly raised

https://images-na.ssl-images-amazon.com/images/I/51VTtKn5-FL._AC_SL1001_.jpg

Quote:

maxheadroom said:

Anyone else have a solid lead on these in the US?  Amazon has Vito's famous deli cups but I think the lids might not be PP5.




Also those Vito's Famous Deli Cups on Amazon says the lids are microwaveable but no PP5 logo. anyone tried those?


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Re: Josex' Guide to No-pour Agar. [Re: bebop]
    #26891910 - 08/21/20 04:37 PM (1 year, 3 months ago)

I wouldn't buy those myself. Reminds me of some I got once that turned out to be some hot garbage. A raised bottom is not that bad, what I don't like about it is that the plastic looks very thin and flimsy, and I would not trust those kind of lids either.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #26891922 - 08/21/20 04:41 PM (1 year, 3 months ago)

Quote:

Josex said:
I wouldn't buy those myself. Reminds me of some I got once that turned out to be some hot garbage. A raised bottom is not that bad, what I don't like about it is that the plastic looks very thin and flimsy, and I would not trust those kind of lids either.




Yeah looks very generic, that exact design is on there 50 times under different brand names.

These look good, just no PP5 logo but the description says Heavy Duty polypropylene and one of the Q&As says its PP5 https://www.amazon.com/dp/B071177PN1/?coliid=I2GD25EO4HYQ6T&colid=1TB3ZA0LKOY2X&psc=1&ref_=lv_ov_lig_dp_it


Edited by bebop (08/21/20 04:42 PM)


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Re: Josex' Guide to No-pour Agar. [Re: bebop]
    #26891931 - 08/21/20 04:46 PM (1 year, 3 months ago)

Have you tried eBay and Aliexpress? I don't like Amazon for this kinda stuff.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #26892075 - 08/21/20 05:58 PM (1 year, 3 months ago)

I have less luck on ebay. Nothing I can find there looks good. I've got the glad rounds though and may just go with those, but I'll need more if I have to do more than a couple transfers

These look good? https://www.aliexpress.com/item/4000346133440.html?spm=a2g0s.8937460.0.0.6cbe2e0eCCpHTW

or https://www.aliexpress.com/item/4000125409443.html?spm=a2g0s.8937460.0.0.6cbe2e0eCCpHTW


Edited by bebop (08/22/20 07:08 PM)


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #26956254 - 09/26/20 05:02 PM (1 year, 2 months ago)

Someone linked me this TEK from another PP5 container thread. Hope it's ok to bump this :smile:

Just checking if you think these might be suitable? I know you mentioned avoiding raised bottoms, but these appear to have a raised circle in the base instead of being flat.

https://www.ebay.com.au/itm/100ml-Plastic-Dipping-Sauce-Disposable-Small-Container-Cups-Lids-Takeaway-Jelly/392919544124

Unfortunately supplies are not easy to come by here in Australia and sometimes we have to make do with close enough alternatives.


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Re: Josex' Guide to No-pour Agar. [Re: Mycodog] * 1
    #26956264 - 09/26/20 05:08 PM (1 year, 2 months ago)

They don't look bad in the pics. Containers with a flat bottom are preferable but you can still use containers with raised bottoms, no problem.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #26956627 - 09/26/20 09:46 PM (1 year, 2 months ago)

Awesome thanks, I will get a set of 100 to start with and see how it goes.

Thanks for the great writeup too!


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Re: Josex' Guide to No-pour Agar. [Re: Mycodog]
    #26956788 - 09/27/20 12:07 AM (1 year, 2 months ago)

So while colonizing do we keep the agar plates out or in the refrigerator? or where/what do we do with them?


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Re: Josex' Guide to No-pour Agar. [Re: GTEED]
    #26977496 - 10/09/20 09:06 PM (1 year, 1 month ago)

Best Agar tek I ever read on here! 5/5 newb stars! :smile: Thanks

I recently tried a no poor tek but a lot of my jars had water in them after, any tips to avoiding excess condensation?

If you add MSS drops to a dish with a bit of condensation water will it fuck it up?


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Re: Josex' Guide to No-pour Agar. [Re: Mtbromo] * 1
    #26977542 - 10/09/20 09:46 PM (1 year, 1 month ago)

Dump excess water on a towel in a sab. Take one drop of mss on a sterile cotton swab and streak it 2 or 3 plates.

Asura has a tek on this.
https://www.shroomery.org/forums/showflat.php/Number/26325679


Pooling is a non issue i dont bother trying to fix. It happens on some plates and does not cause any contamination just messy unorganized cultures.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27099756 - 12/20/20 08:12 PM (11 months, 12 days ago)

Quote:

Using cling wrap to wrap the plates:
If you need to do that because you're getting contamination during colonization then you're most likely using containers that aren't good for this. A good container should not contaminate under normal circumstances without using cling wrap. Also, wrapping them with foil instead is equally useless.




First off great write up, but I have a question for you. Ive been using click wrap for years on my petri plates, simply because its easy and i cut up the roll into 1 or so inches long and it last damn near a year.

So are you saying that using cling wrap is bad practice for petri plates or just these containers, oooor just the fact that people think it will prevent contamination?

Also side note i know you covered this fact already but cant stress enough to make sure the lids are PP5 too. Cant tell you how many times I received a new container to try out just to find out the lid wasn't PP5 and would melt. So if you trying a new kind of container that's not covered here buy a small amount to test first, sometimes they will say the lids are PP5 just to find out they are not and now your stuck with hundreds of containers that we cant use.

Again great write up!


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Re: Josex' Guide to No-pour Agar. [Re: john_conner]
    #27099770 - 12/20/20 08:21 PM (11 months, 12 days ago)

Of course, you need to wrap petris. Was just making a point that if you need to wrap a no-pour plate because they contaminate, then you're not using adequate containers. One of the advantages of using no-pour is that you don't have to wrap them.

Yup, that's sadly a fact. Vendors often tell you the lids are PP5 too when in reality they have no clue. Oftentimes though, lids also have the PP5 icon.


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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27099825 - 12/20/20 09:00 PM (11 months, 12 days ago)

I kind of figured that, but im always looking to improve myself and try new things.
thanks for the response and keep up the good work!


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Re: Josex' Guide to No-pour Agar. [Re: john_conner]
    #27115464 - 12/30/20 07:33 AM (11 months, 2 days ago)

I am currently using some cups that suck and sometimes even warp in the PC I dont even feel safe using them because the lids dont fit tight... But so far have gotten the job done.

Its time for a change though ,did i just hit the jackpot? These look identical to yoursAliexpress deli cups


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InvisibleJosex
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Re: Josex' Guide to No-pour Agar. [Re: Sankhara]
    #27115985 - 12/30/20 12:44 PM (11 months, 2 days ago)

It may work, I'd give them a try, start small. Someone said in the reviews that they were happy with the containers but also that the plastic was thinner than they'd expected.


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OfflineSankhara
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27116120 - 12/30/20 01:46 PM (11 months, 2 days ago)

Thanks! I might buy 20 pieces, larger orders ask for 30 dollar shipping to Argentina :mad:


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Offline9chis
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Re: Josex' Guide to No-pour Agar. [Re: Sankhara]
    #27150137 - 01/15/21 07:04 PM (10 months, 17 days ago)

: bow2:


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Invisiblesavan73
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Re: Josex' Guide to No-pour Agar. *DELETED* [Re: 9chis]
    #27151654 - 01/16/21 05:01 PM (10 months, 16 days ago)

Post deleted by savan73

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Re: Josex' Guide to No-pour Agar. [Re: savan73]
    #27154637 - 01/18/21 05:20 AM (10 months, 14 days ago)

Quote:

savan73 said:
Paper tape is work too? MP tape is expensive.




Where I am, I can only buy MP tape in drugstores and it's terribly expensive too, that's why I buy MP tape (3M brand) in bulk from medical suppliers online, very cheap that way.
Did a big order once for cheap and I'm set for life, so if you're planning on continuing with the hobby I'd consider doing that.

I don't know anything about that paper tape so I can't tell you anything about it.


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Invisiblesavan73
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