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Offlinedeathblade
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Re: Josex' Guide to No-pour Agar. [Re: Sankhara]
    #27184048 - 02/03/21 04:07 PM (2 years, 5 days ago)



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Offlinetheherbaceouslayer
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27184135 - 02/03/21 05:25 PM (2 years, 5 days ago)

Quote:

Josex said:
@theherbaceouslayer, you don't know how many days? 3 days and you can expect some fuckery growing on the plates already.

You could use your PC as a big pot and steam-sterilize them, one hour is plenty.




Thank you for the response 🙏🏻

I expect it would only be a few days before some wack contam finds out there is delicious agar and dextrose in the fridge.

With the steam sterilization, this will work without any PSI? Won't that not be at temp to fully sterilize?

☯️


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OfflineSankhara
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Re: Josex' Guide to No-pour Agar. [Re: deathblade]
    #27184203 - 02/03/21 06:29 PM (2 years, 5 days ago)

Thanks!! I Hope they are durable


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Offlinedeathblade
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Re: Josex' Guide to No-pour Agar. [Re: Sankhara]
    #27184248 - 02/03/21 07:03 PM (2 years, 5 days ago)

They seem good so far. Thick plastic and no warping


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OfflineSankhara
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Re: Josex' Guide to No-pour Agar. [Re: deathblade]
    #27184330 - 02/03/21 08:08 PM (2 years, 5 days ago)

What size did you buy? 6 or 7 cm in diameter? I like the larger ones, they look more like petris, but if 6 does the job just fine then i'll go with that


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InvisibleJosex
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Re: Josex' Guide to No-pour Agar. [Re: theherbaceouslayer]
    #27184474 - 02/03/21 09:34 PM (2 years, 5 days ago)

Quote:

theherbaceouslayer said:
Quote:

Josex said:
@theherbaceouslayer, you don't know how many days? 3 days and you can expect some fuckery growing on the plates already.

You could use your PC as a big pot and steam-sterilize them, one hour is plenty.




Thank you for the response 🙏🏻

I expect it would only be a few days before some wack contam finds out there is delicious agar and dextrose in the fridge.

With the steam sterilization, this will work without any PSI? Won't that not be at temp to fully sterilize?

☯️




Yea you can steam-sterilize agar the same way you can steam PF-TEK cakes, only that agar requires far less time. One hour is really more than enough. I used to steam sterilize agar plates before owning a PC. It is either that or waiting for your weight to arrive and you don't know exactly when that's going to happen.


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Offlinedeathblade
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Re: Josex' Guide to No-pour Agar. [Re: Sankhara]
    #27184504 - 02/03/21 09:51 PM (2 years, 5 days ago)

The 5cm. Very similar size to the old containers i was using just shorter. I may try the wider containers next time but these are a good size to. 500ml of agar filled 60 of them.


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Invisiblesavan73
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Re: Josex' Guide to No-pour Agar. *DELETED* [Re: Josex]
    #27203982 - 02/14/21 01:07 AM (1 year, 11 months ago)

Post deleted by savan73

Reason for deletion: .


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Offlinedeathblade
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Re: Josex' Guide to No-pour Agar. [Re: savan73]
    #27211614 - 02/18/21 02:13 AM (1 year, 11 months ago)

Because of the stated reason of contaminated staying hidden. If you aren't going thru them fast enough just make smaller batches.


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OfflineKingChango
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27287491 - 04/30/21 02:54 PM (1 year, 9 months ago)

I just grabbed some PP5 100mmx15mm dishes from the Amazon, I've found that I get less condensation by pouring them outside the PC, let harden like you said, then wipe the rim of any water vapor. Then I load the PC and run a 45min at 15psi.

I am going to try wrapping each plate in tin foil to help keep moisture out, but that takes up more space. I could always sterilize plates and pour in the SAB once the agar is cool enough to handle and that would avoid condensate. However, it isn't a no pour method, and I prefer no pour. Was using mini-rounds and also some 4oz glass jars that were squat.

However, I am still having condensation on the petris that I do no pour with. Does anyone know how to prevent this? Tried letting PC cool overnight, no luck-still got condensate. I know it's not the end of the world, I'm just looking to get transfers that I can see clearly through the lid.

Is this an unavoidable event when it comes to no pour dishes?


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Offlineodissey
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27480272 - 09/24/21 01:31 PM (1 year, 4 months ago)

Quote:

Josex said:
Fuckin rights man they look legit. <img style="max-width:100%;height:auto;" src="https://files.shroomery.org/smileys/rockon.gif" alt=":rockon:" title=":rockon:"/>

Quote:

that my sterilizer could hold another 60 before its full.




I think so too. I like to put large sheets of foil between the tiers of plates, it helps for building the next layer of plates. It's somewhere in the OP, since they can't be stacked normally in the PC because they get stuck to each other by suction.



Hi Josex! thanks for the write up, this has severely reduced my agar prep time and now I can do 15 at a time which more than satisfies my needs. However, I usually get leakage on the pots so that the agar gets too thin and they get glued together by the leaked agar so I need to clean them afterwards. I skipped the foil layer though. Do you think this is the reason or is it because the lids are not tight? Have you ever had this happen to you? Thanks!


--------------------


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OfflineBonerjamz
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27697735 - 03/16/22 11:19 PM (10 months, 19 days ago)

Dude thanks for the write up
Almost 4 years later


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Offlinehtime99
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27945018 - 09/11/22 06:41 PM (4 months, 26 days ago)

I'm using the GladWare Mini Round 1/2 Cup (4oz) Containers and found that the foil was sticking to them. For anyone else having this same problem, just insert a layer of parchment paper on top of your containers and then the two layers of foil.


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OfflineMadMarchHare
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #28062619 - 11/21/22 10:02 AM (2 months, 18 days ago)

Hi all,

This is my first post in the forum, so I want to say thanks to everyone who is sharing tek and commenting with pointers. This post is basically a description of my process testing Josex's no-pour agar tek, so that if I've done anything foolish I have a reference for my questions, and if I've done anything clever it's on record for the next person.

I've tried to grow mushrooms unsuccessfully twice before now, which ended with contamination both times. I'm trying agar this time because of the evangelism about it that I've seen on here, and my hope is that I can head off contamination issues before transferring to brown rice flour and vermiculite spawn jars. This is also my first time working with a pressure cooker.

I think the toughest part of this was getting everything together. It took a couple tries to find containers that would work for this tek online, but I managed to find a 40 pack of 4oz PP containers online that did the trick. The first set that I ordered came with PET lids; I tested them in the pressure cooker without filling them with agar, and the lids shrivelled up to nothing. It took a lot of staring closely at pictures to get the recycling category 5 PP symbol and feel confident ordering them.

First step once I had everything together was to make some holes in the sides of the containers. You can see in the picture I used a clamp, finishing nail, and blowtorch to make the holes. This worked well.

Next I thought, "I'm going to be smart. I can cut the micropore roll into chunks, then peel off triple layers for my filters and I don't have to make endless cuts. I'm so smart!"

Didn't work.

What worked was using the clamp stem to hold the roll, sticking three layers together as I cut, then sticking the triple to the container. I'm glad my kitchen shears come apart, because micropore is gunky and it was good to be able to clean them fully. As suggested, I numbered all my containers and lids so that I could be sure they'll go through matching pressure cooker cycles together.





I mixed and boiled 500mL of LME agar (500mL water, 9g agar-agar powder, 10g LME). I used tap water. There was some scum on the top of the pot, so I scooped that off with a spoon before pouring. I got 30 plates from that batch; you can see that #31 got the burnt crunchy bits, so I decided to keep that one out of the pressure cooker just to see what contamination was present before sterilization. I think I could have poured a bit thinner and stretched to all 40, but oh well.

30 cups stacked inside my pressure cooker nicely with lots of water space on the bottom. This is one place I deviated from Josex's tek by not using tinfoil on each layer, just because I didn't have any. I followed Josex's advice and just ran the stove at high until it got up to pressure, then turned it down until it was just consistently venting. I ran the pressure cooker for 60 minutes once it hit pressure.





While I waited, I made a still air box as described by bodhisatta. I didn't have any empty cans on hand, so I heated up a box cutter with my blowtorch and cut the holes slowly. It turned out alright, though I'm planning to go back to it once I have a can to make a cleaner cut.

https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888



That only took a short bit of time, so I prepped some brown rice flour and vermiculite jars to sterilize and use later. I used the simplest recipe I could find: 1 cup brown rice flour, 2 cups vermiculite, 1 cup water, x6 to get the jars mostly full. I tamped them down with a smaller jar, wiped the rims, and put them into the next pressure cooker cycle. I have some experience canning fruit, so with fully sealed negative pressure jars I'm expecting to see these stay clean until I open them to add chunks of agar later.



The agar containers held up in the pressure cooker just fine. The lack of tinfoil meant that some of the upper containers had suctioned to the lids below, so when removing them I had to be careful to put a finger on the lower lid as I lifted. They didn't take long to cool and set. I transferred to my still air box and added a couple drops from a spore syringe to inoculate. I didn't have a lighter small enough to work in the box (just the blowtorch!) so I wasn't able to flame sterilize the needle before each plate. Fingers crossed that doesn't cause any issues. I inoculated five plates with each of the two syringes I have, so I've got 20 left over and sterile for making transfers before going to the jars.



For timing, it was about 2 hours total work to go from assembled materials to turning the stove on to sterilize the plates. I'm sure it'll be less on future runs. It was another 3 hours total to get the jars in the pressure cooker and complete the still air box work. The tasks and wait times for doing the plates and the jars in parallel worked out fairly well. I figure I can get a total of 60 plates in my pressure cooker if I reduce the amount of water in the bottom and stack them in 6 layers of 10 plates each. That would mean making 1000mL of agar. I regret slightly not getting an overabundance of containers as suggested, but it was the right choice to play it safe until I was confident that the containers I had ordered would work in the pressure cooker.

I'll post an update soon about the state of the agar and jars.


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InvisibleJosex
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Re: Josex' Guide to No-pour Agar. [Re: MadMarchHare]
    #28063429 - 11/22/22 12:11 AM (2 months, 17 days ago)

Damn, what a detailed rundown! Gotta love the passion beginners have. It shows in your post and it takes me back to when I was a newbie myself... it would all feel like fucking magic.

Those containers don't look bad from here and I think you'll be fine. Good luck!


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InvisibleMr. Anderson
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Re: Josex' Guide to No-pour Agar. [Re: MadMarchHare]
    #28088001 - 12/08/22 06:09 AM (2 months, 1 day ago)

Hey MadMarchHare, that container you have the electrical tape in that is similar in shape to a petri dish next to your propane canister in the first picture you posted are usually pp5 and can make good agar dishes lol.

Not much else to add besides wondering if anyone has a remedy for the condensation that builds up in the dishes doing no pour. Btw nice write up Josex.:mushroom2:


--------------------


Disclaimer: All posts are completely fictional and or for educational purposes only.


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OfflinePhilberto
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #28101520 - 12/17/22 10:25 AM (1 month, 23 days ago)

Gotta say thanks for the knowledge.
I went from 20% contamination rate using Petri dishes, to literally zero using your method in these portion cups.
I did a whole pressure cooker full, 130+ and literally no failures.
I finally got clean myc and I'm ready to progress.
Couldn't move forward til I got my agar game right.
Using eBay item 195490045718 if anyone needs some.


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