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Offlinedeathblade
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Re: Josex' Guide to No-pour Agar. [Re: Sankhara]
    #27184048 - 02/03/21 09:07 AM (9 months, 22 days ago)



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Offlinetheherbaceouslayer
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27184135 - 02/03/21 10:25 AM (9 months, 22 days ago)

Quote:

Josex said:
@theherbaceouslayer, you don't know how many days? 3 days and you can expect some fuckery growing on the plates already.

You could use your PC as a big pot and steam-sterilize them, one hour is plenty.




Thank you for the response 🙏🏻

I expect it would only be a few days before some wack contam finds out there is delicious agar and dextrose in the fridge.

With the steam sterilization, this will work without any PSI? Won't that not be at temp to fully sterilize?

☯️


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OfflineSankhara
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Re: Josex' Guide to No-pour Agar. [Re: deathblade]
    #27184203 - 02/03/21 11:29 AM (9 months, 22 days ago)

Thanks!! I Hope they are durable


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Offlinedeathblade
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Re: Josex' Guide to No-pour Agar. [Re: Sankhara]
    #27184248 - 02/03/21 12:03 PM (9 months, 21 days ago)

They seem good so far. Thick plastic and no warping


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OfflineSankhara
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Re: Josex' Guide to No-pour Agar. [Re: deathblade]
    #27184330 - 02/03/21 01:08 PM (9 months, 21 days ago)

What size did you buy? 6 or 7 cm in diameter? I like the larger ones, they look more like petris, but if 6 does the job just fine then i'll go with that


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InvisibleJosex
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Re: Josex' Guide to No-pour Agar. [Re: theherbaceouslayer]
    #27184474 - 02/03/21 02:34 PM (9 months, 21 days ago)

Quote:

theherbaceouslayer said:
Quote:

Josex said:
@theherbaceouslayer, you don't know how many days? 3 days and you can expect some fuckery growing on the plates already.

You could use your PC as a big pot and steam-sterilize them, one hour is plenty.




Thank you for the response 🙏🏻

I expect it would only be a few days before some wack contam finds out there is delicious agar and dextrose in the fridge.

With the steam sterilization, this will work without any PSI? Won't that not be at temp to fully sterilize?

☯️




Yea you can steam-sterilize agar the same way you can steam PF-TEK cakes, only that agar requires far less time. One hour is really more than enough. I used to steam sterilize agar plates before owning a PC. It is either that or waiting for your weight to arrive and you don't know exactly when that's going to happen.


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Offlinedeathblade
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Re: Josex' Guide to No-pour Agar. [Re: Sankhara]
    #27184504 - 02/03/21 02:51 PM (9 months, 21 days ago)

The 5cm. Very similar size to the old containers i was using just shorter. I may try the wider containers next time but these are a good size to. 500ml of agar filled 60 of them.


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Invisiblesavan73
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Re: Josex' Guide to No-pour Agar. *DELETED* [Re: Josex]
    #27203982 - 02/13/21 06:07 PM (9 months, 11 days ago)

Post deleted by savan73

Reason for deletion: .


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Offlinedeathblade
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Re: Josex' Guide to No-pour Agar. [Re: savan73]
    #27211614 - 02/17/21 07:13 PM (9 months, 7 days ago)

Because of the stated reason of contaminated staying hidden. If you aren't going thru them fast enough just make smaller batches.


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OfflineKingChango
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27287491 - 04/30/21 07:54 AM (6 months, 29 days ago)

I just grabbed some PP5 100mmx15mm dishes from the Amazon, I've found that I get less condensation by pouring them outside the PC, let harden like you said, then wipe the rim of any water vapor. Then I load the PC and run a 45min at 15psi.

I am going to try wrapping each plate in tin foil to help keep moisture out, but that takes up more space. I could always sterilize plates and pour in the SAB once the agar is cool enough to handle and that would avoid condensate. However, it isn't a no pour method, and I prefer no pour. Was using mini-rounds and also some 4oz glass jars that were squat.

However, I am still having condensation on the petris that I do no pour with. Does anyone know how to prevent this? Tried letting PC cool overnight, no luck-still got condensate. I know it's not the end of the world, I'm just looking to get transfers that I can see clearly through the lid.

Is this an unavoidable event when it comes to no pour dishes?


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Offlineodissey
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Registered: 01/19/21
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Re: Josex' Guide to No-pour Agar. [Re: Josex]
    #27480272 - 09/24/21 06:31 AM (2 months, 6 days ago)

Quote:

Josex said:
Fuckin rights man they look legit. <img style="max-width:100%;height:auto;" src="https://files.shroomery.org/smileys/rockon.gif" alt=":rockon:" title=":rockon:"/>

Quote:

that my sterilizer could hold another 60 before its full.




I think so too. I like to put large sheets of foil between the tiers of plates, it helps for building the next layer of plates. It's somewhere in the OP, since they can't be stacked normally in the PC because they get stuck to each other by suction.



Hi Josex! thanks for the write up, this has severely reduced my agar prep time and now I can do 15 at a time which more than satisfies my needs. However, I usually get leakage on the pots so that the agar gets too thin and they get glued together by the leaked agar so I need to clean them afterwards. I skipped the foil layer though. Do you think this is the reason or is it because the lids are not tight? Have you ever had this happen to you? Thanks!


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