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Invisiblemindblowing
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Registered: 12/26/15
Posts: 238
Few questions regarding making spore syringes
    #25136546 - 04/13/18 06:40 PM (6 years, 9 months ago)

Ofcouse ive read every thread i can find, teks, and videos seems quiet easy but i just want to clear a few things up.

Alright so, one thing i want to clear up is the syringes in packets so to speak sterlie already correct, so its just the water ive got to sterlize from what i can gather distlied will last longer thats all?

The problem is im trying to solve is i dont want to pc for 1.5hrs just for a jar of water, so im guessing syringes out of the packets dont really need to be sterlized.

The threads ive all been reading are 10 years old, video from this year and he pc's the water still to this day.

Will boiling my distiled water be good enough way to sterlize the water? Then put the boiling water in my syringe a few times, wipe my shotglass cup etc with rubbibg alochol. Id rather pc if boiling water is a risky as way, i dont see why that wouldnt be sterlie enough? I just need rensurance on this infomation sorry, dont want to waste all this time doing it dodgy, thankyou


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InvisibleAdden
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Re: Few questions regarding making spore syringes [Re: mindblowing] * 1
    #25136568 - 04/13/18 06:47 PM (6 years, 9 months ago)

You should only have to PC a quart of water for 15 minutes. Boiling water and all the stuff that falls in it is the gamble.

All prints are dirty anyway. Why bother with syringes? You can just as easily do no-pour agar, clean it up, PC loaded syringes, and use LI if you're making cakes. Cuts the cake time down to 16 days.

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Invisiblemindblowing
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Re: Few questions regarding making spore syringes [Re: Adden]
    #25136629 - 04/13/18 07:03 PM (6 years, 9 months ago)

Thanks mate. So what 15 minutes after it reaches 15psi?

Well i havent got any petrie dish's and i pced my grains a day ago, ill look into non pour see if ill have the time to do it.

I already have agar at home planned to get onto it asap after syringe maybe i should just skip syringes?


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Invisiblemindblowing
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Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25136676 - 04/13/18 07:23 PM (6 years, 9 months ago)

Just from a bit of research with non pour, I still have to make a syringe regardless to inoculate the the agar?


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OfflinehamloafM
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Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25136697 - 04/13/18 07:32 PM (6 years, 9 months ago)

No, agar can be inoculated directly from spore print in sterile conditions using an inoculation loop or something of the sort.


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Invisibleelasticaltiger
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Re: Few questions regarding making spore syringes [Re: hamloaf]
    #25136762 - 04/13/18 07:58 PM (6 years, 9 months ago)

OP for spore prints and agar I buy pre sterilized swabs (10$ for a hundred I think)

I rub the print with the swab and then i touch the swab to the agar to deposite some spores.


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First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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OfflineCrispy224
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Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25136815 - 04/13/18 08:12 PM (6 years, 9 months ago)

Quote:

mindblowing said:
Ofcouse ive read every thread i can find, teks, and videos seems quiet easy but i just want to clear a few things up.

Alright so, one thing i want to clear up is the syringes in packets so to speak sterlie already correct, so its just the water ive got to sterlize from what i can gather distlied will last longer thats all?

The problem is im trying to solve is i dont want to pc for 1.5hrs just for a jar of water, so im guessing syringes out of the packets dont really need to be sterlized.

The threads ive all been reading are 10 years old, video from this year and he pc's the water still to this day.

Will boiling my distiled water be good enough way to sterlize the water? Then put the boiling water in my syringe a few times, wipe my shotglass cup etc with rubbibg alochol. Id rather pc if boiling water is a risky as way, i dont see why that wouldnt be sterlie enough? I just need rensurance on this infomation sorry, dont want to waste all this time doing it dodgy, thankyou



I've used boiled water right out the tap. I just sucked in the water 3-4 times and left the water in the syringe the last time and used it. Worked fine for me. But maybe I just got lucky. I know we pressure cook because certain things can survive being boiled.


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Invisiblemindblowing
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Re: Few questions regarding making spore syringes [Re: elasticaltiger]
    #25136866 - 04/13/18 08:30 PM (6 years, 9 months ago)

Quote:

hamloaf said:
No, agar can be inoculated directly from spore print in sterile conditions using an inoculation loop or something of the sort.



Quote:

elasticaltiger said:
OP for spore prints and agar I buy pre sterilized swabs (10$ for a hundred I think)

I rub the print with the swab and then i touch the swab to the agar to deposite some spores.




Ok thanks guy, I think i might go that route.

im looking at sterile swabs now, I'm just worried my grains sitting around after PC'ing. I know agar would be the way to go I've always known this i should of started my agar weeks ago, i just don't want to push the time on my grains now that I've already done so.

Okay i'm pretty certain i'm going make up some agar plates regardless as i'll PC my syringe water + agar non pour, But here's the problem I've got grain ready right now & 2-4 weeks for colonizing time for agar as i'm reading.

Soo, Should i just knock up a syringe for my grains I've got ready right now? Start some agar for my next batch in a month? honestly what else can i do i think that's my best situation, agar plates won't be ready for a while i don't want to stuff this grain, made up 2quart worth


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Invisiblemindblowing
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Posts: 238
Re: Few questions regarding making spore syringes [Re: Crispy224]
    #25136872 - 04/13/18 08:32 PM (6 years, 9 months ago)

Quote:

Crispy224 said:
Quote:

mindblowing said:
Ofcouse ive read every thread i can find, teks, and videos seems quiet easy but i just want to clear a few things up.

Alright so, one thing i want to clear up is the syringes in packets so to speak sterlie already correct, so its just the water ive got to sterlize from what i can gather distlied will last longer thats all?

The problem is im trying to solve is i dont want to pc for 1.5hrs just for a jar of water, so im guessing syringes out of the packets dont really need to be sterlized.

The threads ive all been reading are 10 years old, video from this year and he pc's the water still to this day.

Will boiling my distiled water be good enough way to sterlize the water? Then put the boiling water in my syringe a few times, wipe my shotglass cup etc with rubbibg alochol. Id rather pc if boiling water is a risky as way, i dont see why that wouldnt be sterlie enough? I just need rensurance on this infomation sorry, dont want to waste all this time doing it dodgy, thankyou



I've used boiled water right out the tap. I just sucked in the water 3-4 times and left the water in the syringe the last time and used it. Worked fine for me. But maybe I just got lucky. I know we pressure cook because certain things can survive being boiled.




Thanks mate, Yeah i think PC would be the safest option.

I'm thinking, besides wasting doing a pc cycle, i'll grab some supplies get me going some agar + syringe for my current batch of grain..


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Invisibleelasticaltiger
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Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25136887 - 04/13/18 08:38 PM (6 years, 9 months ago)

I guess if time is a factor then making a syringe would be faster than waiting for agar to grow out.

Just be as clean as possible and don't be surprised if your first syringe is contaminated.

The best way to make clean syringes is to grow invitro and print invitro fruits.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Invisiblemindblowing
Stranger Danger

Registered: 12/26/15
Posts: 238
Re: Few questions regarding making spore syringes [Re: elasticaltiger]
    #25136925 - 04/13/18 08:52 PM (6 years, 9 months ago)

Quote:

elasticaltiger said:
I guess if time is a factor then making a syringe would be faster than waiting for agar to grow out.

Just be as clean as possible and don't be surprised if your first syringe is contaminated.

The best way to make clean syringes is to grow invitro and print invitro fruits.




Thanks for the reply & the infomation.

Hmm, I can't remember how many syringes i brought, I might try make 2-3 syringes and mix it around a bit for my jars, I dont want to make 1 syringe and containment all 8 jars lol :frown: really hope i get some success

True about invitro for syringes, I think i'll just learn my lesson hope for the best and move onto agar asap...


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Invisiblemindblowing
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Registered: 12/26/15
Posts: 238
Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25137169 - 04/13/18 10:28 PM (6 years, 9 months ago)

Im in a mall looking for agar plates

In my country we dont have perfect containers like the usa i will get petrie soon

Would these little glass be okay for non pour? dosent really matter does it just a nice little jar or bpa free container?


Only problem is these add up quickly price wise but i cant find anywhere decent little plastic containers bpa free


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Edited by mindblowing (04/13/18 10:31 PM)

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InvisibleZiran
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Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25137185 - 04/13/18 10:36 PM (6 years, 9 months ago)

you need wide mouths for pf tek. tapered are impossible to birth without a hammer.


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Invisiblemindblowing
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Posts: 238
Re: Few questions regarding making spore syringes [Re: Ziran]
    #25137227 - 04/13/18 11:00 PM (6 years, 9 months ago)

Okay very true i just found some containers they are perfect but in chibese i cant read them they say microwave safe, 140c but then say with lid off


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Invisiblemindblowing
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Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25137228 - 04/13/18 11:01 PM (6 years, 9 months ago)

So im not sure if they will be safe to pc very confused


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OfflinehamloafM
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Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25137237 - 04/13/18 11:06 PM (6 years, 9 months ago)

Should say PP5 with a triangle made of arrows around it if it's autoclave able somewhere on container.  However, 140 degrees Celsius is well beyond 15psi, so they'll be fine to PC.  Whether lid is on, or off won't make a difference because lids for PF Tek contain holes thus pressure won't build up in containers, and they won't burst.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Invisiblemindblowing
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Registered: 12/26/15
Posts: 238
Re: Few questions regarding making spore syringes [Re: hamloaf]
    #25137328 - 04/14/18 12:16 AM (6 years, 9 months ago)

Quote:

hamloaf said:
Should say PP5 with a triangle made of arrows around it if it's autoclave able somewhere on container.  However, 140 degrees Celsius is well beyond 15psi, so they'll be fine to PC.  Whether lid is on, or off won't make a difference because lids for PF Tek contain holes thus pressure won't build up in containers, and they won't burst.




Thanks for that man i went and grabbed some better quailty containers they should be fine

Im not using them with pf tek though, they are for my cultures


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Offlineverum subsequentis
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Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25137352 - 04/14/18 12:45 AM (6 years, 9 months ago)

i caught that everyone was missing the fact that you wanted them for agar and not pf. anything that can withstand the pc (glass or pp5) will work but you want something you can see the culture through and that is shaped in a way that will be easy to transfer out of.

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Invisiblemindblowing
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Re: Few questions regarding making spore syringes [Re: verum subsequentis]
    #25137464 - 04/14/18 02:07 AM (6 years, 9 months ago)

Quote:

verum subsequentis said:
i caught that everyone was missing the fact that you wanted them for agar and not pf. anything that can withstand the pc (glass or pp5) will work but you want something you can see the culture through and that is shaped in a way that will be easy to transfer out of.




Thanks heaps you cleared up all my questions, my containers have a traingle with 5 in them, id say they are perfect!

Thanks everyone im going to knock up some agar,

Hey just after some personal perference, how do we like to inncoulate our agar? Spore syringe, just scrape some spores with a knife or sterile cotton tips, just asking as sterile cotton tips are hard to get here, and syringes are an increased risk, many people just scrape a few in?


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Edited by mindblowing (04/14/18 02:09 AM)

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Offlineverum subsequentis
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Re: Few questions regarding making spore syringes [Re: mindblowing]
    #25138291 - 04/14/18 12:01 PM (6 years, 8 months ago)

you can do it all sorts of ways. basically if you scrape anything sterile across your print and then across the agar in a few places, you'll probably get more than enough spores.

you can pressure cook a few q tips in foil for fifteen minutes and then you've made your own sterile swabs. just don't get the plastic q tips.

i'd probably make a syringe and put it to grain and just accept the fact that I'm a noob and this will probably not work. but give it your best and it might.
it's not a big deal to toss a couple jars and start again. Most of us have tossed absolute shitloads of grain in our days.

also it's very simple to make your own loop. loop's are great because they sterilize very easily with a flame and are great for streaking a spore drop across your plate.

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