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OfflineFishLevelMidnightS
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is BRF (paste or jars or whatever) more forgiving towards bacteria?
    #25135964 - 04/13/18 04:22 PM (3 years, 29 days ago)

I remember reading somewhere that brf with verm is often used with spore solutions because it is more forgiving against bacterial contamination (something about the high ratio of non-nutritive verm helps fungi get a foothold over bacteria; I am not saying it is impossible to have bacterial contam problems using brf cakes/paste). Something about the non-nutritive verm in the brf...

Can't seem to find anything with the proper buzz words. I think I remember it best in reference to brf paste and using that to outrun bacteria then moving to agar or whatever.

Any help to links or experiences with bacterial syringes (brf vs grain) would be much appreciated!


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InvisiblebodhisattaM
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Re: is BRF (paste or jars or whatever) more forgiving towards bacteria? [Re: FishLevelMidnight] * 1
    #25136025 - 04/13/18 04:45 PM (3 years, 29 days ago)

I would say its harder for bacteria to move in brf cakes so contamination stays localized. But to say bacteria doesn't have an easy time on brf is totally wrong, and the verm being nutritional or non nutritional has nothing to do with it.

Bacteria doesn't move like mold. Even motile bacteria need an aquious medium to get around. Grain jars get shaken and dispersed so that's the problem there. On a cake a small bacterial contamination can usually be washed off.


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Re: is BRF (paste or jars or whatever) more forgiving towards bacteria? [Re: bodhisatta]
    #25136029 - 04/13/18 04:48 PM (3 years, 29 days ago)

Thanks for the clarification!

That makes sense to me. :cheers:


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Re: is BRF (paste or jars or whatever) more forgiving towards bacteria? [Re: FishLevelMidnight]
    #25136047 - 04/13/18 04:56 PM (3 years, 29 days ago)

Even on a petri dish bacteria usually doesn't get too far. Motioe bacteria make bigger patches usually odd shaped too but still don't colonize an entire dish that ive seen. Unless you give the bacteria a helping hand by shaking or whatnot. But its generally a small threat to cakes by nature of how sponge like they are


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