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OfflineGrimsweeper
don't fear the sweeper
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Registered: 01/29/18
Posts: 4,870
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Last seen: 10 days, 16 hours
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Questions on isolates
    #25135396 - 04/13/18 10:19 AM (6 years, 9 months ago)

Hi all -
I started a few months ago with Golden Teacher spores that had some nice flushes and potency was great. Since then I’ve used the original cake myc for grain and several G2G transfers. My question is have I been diminishing any of the original traits I started with? Without screwing anything up could I wind up with a crappy flush based on genetics alone using the same myc cut down over and over? Also, I’m moving to agar next using a biopsy (again from the original source). Other than “ropey” myc what am I looking for to isolate to insure the same or better positive traits I began with?


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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OG Cultivator
Re: Questions on isolates [Re: Grimsweeper]
    #25136323 - 04/13/18 05:07 PM (6 years, 9 months ago)

You're gonna run into senescence expanding the same culture by G2G. Take a spore print and get a clone to run with. Save cultures on slants.


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bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Questions on isolates (moved) [Re: Grimsweeper]
    #25138158 - 04/14/18 10:58 AM (6 years, 8 months ago)

This thread was moved from Advanced Mycology.

Reason:
Not advanced

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