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OfflineA.k.aM
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Re: Cannabis Growers [Re: Bph] * 2
    #27679650 - 03/02/22 11:57 AM (1 year, 10 months ago)

Brainbulb that last grow looks pretty good too, that middle cola is huge.



Strawberry pie nug and Laos clone budding.



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OfflineBrain Bulb
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Re: Cannabis Growers [Re: Bph]
    #27679652 - 03/02/22 11:58 AM (1 year, 10 months ago)

@Bph

What kind of soil/amendments are you using?  Definitely don’t want the tent to get too hot.  That happened to me a couple times on my last run and I lost a lot of terpines.


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InvisibleBph
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Re: Cannabis Growers [Re: Bph]
    #27679656 - 03/02/22 12:03 PM (1 year, 10 months ago)

The soil is a recipe I’ve been using for container plants for a while. It’s composted cow manure, Pete moss, aged pine bark, perlite, sandy loam, oyster shell, Fish meal, crab meal, shrimp meal, earthworm castings, fossilized bat guano, kelp meal. It’s always done me well I’ve never had these kind of problems out of tomatoes or peppers. They love it.


Edited by Bph (03/02/22 12:06 PM)


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Invisible420rydaz69me
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Re: Cannabis Growers [Re: Brain Bulb]
    #27679669 - 03/02/22 12:20 PM (1 year, 10 months ago)

You can def lose some of those finer terpenes even in the mid-high 80s



Quote:

Enjoil said:

If you are constantly having to adjust your ph… something is wrong.





Not necessarily. If you don't use all your feed and it sits over night it def can climb up tenths or even points depending on what you're using.
When I was working with some coco ppl before you def had to readjust on a 250/300gal reservoir.



@Lotkid - blue lab is top of the line, had a ph tester for about 5 yrs and its finally crapping out on me, so I picked up another to confirm and have been re-calibrating the original once a month roughly. They are expensive tho.
One down side I'd say to a drip system in my mind is adding too much up or down over the course of not being in use, you want to keep that stuff to a minimum in water. My personal opinion tho.



Quote:

Brain Bulb said:
Those look great.  What kind of ferments are you using?  I’ve never used them but wouldn’t mind trying.




Thanks homie!
You can use basic dry nutrients in pantyhose/paint strainer(sometimes double them up) and throw them in your water drum with a bubbler.
Sometimes I bubble for a few days, sometimes a week, sometimes two weeks, depending how long you can handle the smell :meanlaugh:
Kelp, bat guano, neem, crab meal, fish bone meal, fish hydrolysate, minerals and whatnot such as gypsum, langbeinite rock phosphate, humic acid and basically most other dry nutrients
Molasses, milk, coconut milk, worm castings, manure, pee, even just a scoop of soil from a nearby wooded area, you're trying to improve beneficial bacteria
You can also make ferments from biodynamic accumulators, this link has a spread sheet of a large list:
http://www.waldeneffect.org/blog/List_of_dynamic_accumulators/
I grow lots of medicinal herbs, so during the warm months I tend to use comfrey, nettle, wild flowers, sage, rosemary, lavender, garlic, and so many other plants I cant even think of right now.
Hope that helps, do some exploration of your own, the plants love it and the final product tastes far superior than something hit with "the terpinator" or some terp enhancement product that makes every strain taste the same


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InvisibleBph
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Re: Cannabis Growers [Re: A.k.a]
    #27679688 - 03/02/22 12:43 PM (1 year, 10 months ago)

Quote:

A.k.a said:
Yeah they won’t enlarge for me either. I don’t think I’ve seen that before.


Does that 4x8 tent have a divider in the middle??





Yeah but it’s not light tight tho so it’s kind of useless.


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OfflineEnjoil
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Re: Cannabis Growers [Re: 420rydaz69me]
    #27679707 - 03/02/22 12:57 PM (1 year, 10 months ago)

We are talking about running ebb and flow tables bro bro. A recirculating system….


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OfflineBrain Bulb
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Re: Cannabis Growers [Re: Bph]
    #27679736 - 03/02/22 01:25 PM (1 year, 10 months ago)

Quote:

Bph said:
The soil is a recipe I’ve been using for container plants for a while. It’s composted cow manure, Pete moss, aged pine bark, perlite, sandy loam, oyster shell, Fish meal, crab meal, shrimp meal, earthworm castings, fossilized bat guano, kelp meal. It’s always done me well I’ve never had these kind of problems out of tomatoes or peppers. They love it.




Outside of my wheel house atm.  Someone with more experience will have to help.


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Edited by Brain Bulb (03/02/22 03:36 PM)


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OfflineDoc9151M
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Re: Cannabis Growers [Re: Brain Bulb]
    #27679790 - 03/02/22 02:45 PM (1 year, 10 months ago)

Quote:

Icon said:
Yes and most brands like Promix and Foxfarm use trichoderma too. It's one reason my mushroom hobby died when I started cultivating. Anyone else notice more myc contamination when they started growing indoors?



Nope, you should change clothes and clean yourself up though before going from cannacult to mushcult. Never print or dry wild mush in or near the mushcult area or you risk nasty infestations. That's how I ended up with mutant specops spider mites that drank neem for fun and shot fire out their butts!!!

Dr. Pinkshrooms, Low relative humidity will cause water loss in the reservoir.

One of the biggest things that I recommend is regularly calibrating your ph and ec/ppm pens. I check mine with calibration fluids once a month or anytime I notice a swing in readings, because I've been doing it long enough to know what usually comes out of tap for ph, ec/ppm I'm still learning about.


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Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


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OfflineA.k.aM
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Re: Cannabis Growers [Re: Doc9151]
    #27679819 - 03/02/22 03:13 PM (1 year, 10 months ago)

Man that terpene loss worries me. For like 4 months the only place below 80 here is the fridge.


This might finally push me into buying a second one. A combo bud/slant fridge :lol:


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LAGM2020


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OfflineBrain Bulb
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Re: Cannabis Growers [Re: A.k.a]
    #27679853 - 03/02/22 03:51 PM (1 year, 10 months ago)

Quote:

A.k.a said:
Brainbulb that last grow looks pretty good too, that middle cola is huge.



Strawberry pie nug and Laos clone budding.






Thanks!  I was happy with the progress I made with that grow but I made a lot of mistakes.  Ventilation wasn’t set up right so there were a few days the tent got into the upper 90’s while I was at work and I lost a lot of terpines.  The flower smokes clean and gets you stoned but has very little smell/flavor…. The main thing I’m working on this run is controlling my environment. 

Looking good on your end.  How’s that Strawberry Pie?


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OfflineA.k.aM
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Re: Cannabis Growers [Re: Brain Bulb]
    #27679972 - 03/02/22 05:35 PM (1 year, 10 months ago)

It’s really good but I overdid it with the seeds.


Luckily I left the main cola untouched but a lot of the lowers have so many seeds there’s not really any bud.


But on the other hand I have a healthy supply of f1s to work with.


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OfflineDoc9151M
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Re: Cannabis Growers [Re: A.k.a] * 1
    #27680003 - 03/02/22 05:58 PM (1 year, 10 months ago)

I thought this was an interesting read, it's something I've been curious about but never really looked into much, some of you are already familiar with the information judging by your comments.

Abstract
Cannabis (Cannabis sativa L.) is typically propagated using stem cuttings taken from mother plants to produce genetically uniform propagules. However, producers anecdotally report that clonal lines deteriorate over time and eventually produce clones with less vigor and lower cannabinoid levels than the original mother plant. While the cause of this deterioration has not been investigated, one potential contributor is the accumulation of somatic mutations within the plant. To test this, we used deep sequencing of whole genomes (>50×) to compare the variability within an individual cannabis cultivar Honey Banana plant sampled at the bottom, middle, and top. We called over six million sequence variants based on a reference genome and found that the top had the most by a sizable amount. Comparing the variants among the samples uncovered that nearly 600,000 (34%) were unique to the top while the bottom only contained 148,000 (12%), and middle with 77,000 (9%) unique variants. Bioinformatics tools were used to identify mutations in critical cannabinoid-terpene biosynthesis pathways. While none were identified as high impact, four genes contained more than double the average level of nucleotide diversity (π) in or near the gene. Two genes code for essential enzymes required for the cannabinoid pathway while the other two are in the terpene pathways, demonstrating that mutations were accumulating within these pathways and could influence their function. Overall, a measurable number of intraplant genetic diversity was discovered that could impact long-term genetic fidelity of clonal lines and potentially contribute to the observed decline in vigor and cannabinoid content.

Citation:
Adamek K, Jones AMP, Torkamaneh D. Accumulation of somatic mutations leads to genetic mosaicism in cannabis. Plant Genome. 2021 Nov 22:e20169. doi: 10.1002/tpg2.20169. Epub ahead of print. PMID: 34806848.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


Edited by Doc9151 (03/02/22 05:58 PM)


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InvisibleHFM
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Re: Cannabis Growers [Re: Doc9151]
    #27680131 - 03/02/22 07:47 PM (1 year, 10 months ago)

How much of this do you think has to do with lighting in there samples? You can get a variable (any mutation) in cell division from top,middle,and bottom through a lot of mechanisms but,how much do you think has to do with environmental factors? Do you think passing things like variagation will lead to a rapid loss of expression?


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Trees lay chipped across the paths,
we hunt their souls to eat the wrath-HFM


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Offlinespirit_shadow
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Re: Cannabis Growers [Re: HFM] * 1
    #27680153 - 03/02/22 08:10 PM (1 year, 10 months ago)

I just had a mandarin cookies v3 pop up yesterday(gonna be an outdoor plant) :excited:


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InvisibleHFM
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Re: Cannabis Growers [Re: spirit_shadow]
    #27680158 - 03/02/22 08:18 PM (1 year, 10 months ago)

That's rad! :smile:
Have you run any of the mandarin cookies outside before?


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Trees lay chipped across the paths,
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InvisibleLotKid
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Re: Cannabis Growers [Re: HFM]
    #27680277 - 03/02/22 10:23 PM (1 year, 10 months ago)

I have to ph my water down. If i leave it sit in a res for a day or two... it creeps back up. Its just how the world works. If that wasnt the case then ph contollers wouldnt be a thing


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
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InvisibleLizardWizard
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Re: Cannabis Growers [Re: HFM] * 1
    #27680457 - 03/03/22 03:19 AM (1 year, 10 months ago)

Quote:

HFM said:
How much of this do you think has to do with lighting in there samples? You can get a variable (any mutation) in cell division from top,middle,and bottom through a lot of mechanisms but,how much do you think has to do with environmental factors? Do you think passing things like variagation will lead to a rapid loss of expression?





In all my experience with variegated and whorled specimens, it has always shown whorled specimens and variegated specimens to be weaker than their non-mutant sisters.

Not so long ago someone here mentioned they had noticed that their clones remained viable and vigorous for more generations when taken from the mid sections of the plant.

This piece of evidence was just what I was hoping on. I already started taking cuttings more often from the bottom and middle parts as it did feel right to me, I just didn't really know why it felt right yet.

:themoreyouknow:


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InvisibleLizardWizard
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Re: Cannabis Growers [Re: LizardWizard]
    #27680493 - 03/03/22 04:34 AM (1 year, 10 months ago)

Just thought about another interesting thing my literature on the matters mentions, that even though a whorled structure plant delivers bud of lower potency, neither the whorled growth nor the lower potency are passed on to offspring when those plants are used for seed.

I don't know how true this is, though I'm pretty sure whorled growth and also triploid or tetraploid is something that you can encounter across the entire spectrum of sativa/indica/ruderal.

Drawing from my own experience I would say there is a higher chance in some strains for aberrant growth, but that's not triploid, tetraploid or whorled structure. Those I think I've seen pretty even across the spectrum.


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InvisibleHFM
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Re: Cannabis Growers [Re: LizardWizard]
    #27680529 - 03/03/22 05:54 AM (1 year, 10 months ago)

So can somatic mutations account for an evolution of the whole set of chromosomes or just part of a group?Then how much of that is environment dependant?Do you think it could be replicated in a beneficial manner?Is it to help protect itself from a learned or experienced stress or just the cause from that stress?


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Trees lay chipped across the paths,
we hunt their souls to eat the wrath-HFM


Edited by HFM (03/03/22 06:59 AM)


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InvisibleLizardWizard
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Re: Cannabis Growers [Re: HFM] * 1
    #27680581 - 03/03/22 07:05 AM (1 year, 10 months ago)

I think stress is behind the mutations much in the same way that humans and other organisms also experience detriment from stress. It's even a very important driver of autoimmune diseases.

It's the derangement of an organism resulting from the stress that the organism experienced trying to maintain homeostasis in an environment or at an age that didn't properly support that homeostasis.

A bit more nuanced, an organism has ways to cope with things but those ways also have a cost to them.

Is there a way to avoid this from taking place? Perhaps, but I doubt it's feasible to ever do so in practice.

Best we can do in my view is try to gain an understanding of life and work with the possibilities within that understanding, rather than trying to alter the way life inherently works.


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The best things in life
can be smelled on one's fingers.


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