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OfflineAmanitus
Earning my chops
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Registered: 03/31/13
Posts: 167
Loc: Buenos Aires
Last seen: 2 years, 4 months
Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: TrappinInNature]
    #25128729 - 04/10/18 03:10 PM (5 years, 10 months ago)

Quote:

TrappinInNature said:
Remember when loading jars, you want it loose and fluffy.

NO packing. Except to even it out at the top.





I got that part allright!

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OfflineAmanitus
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Loc: Buenos Aires
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: elasticaltiger]
    #25128746 - 04/10/18 03:14 PM (5 years, 10 months ago)

Quote:

elasticaltiger said:
How old are your spores? You said you're making syringes? How are you making them?




I made the syringe out of a spore print that was 5 years old. It had been kept inside a mini ziplock bag inside a lunch box inside a plastic box in a warehouse.

I made the syringe inside a SAB. I scraped some specks of the spore print into a sanitized shot glass (with hand sanitizer instead of alcohol, i know) and then i took a sterile disposable syringe and sucked 10 mls of mineral water. I dumped that water in the shot glass, re-sucked it and dumped it again in the glass a couple of times. Finally i flame sterilized the needle and put the cap on.

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InvisibleZiran
The Hero of Time
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Amanitus]
    #25128780 - 04/10/18 03:24 PM (5 years, 10 months ago)

I just sterlize tap water in a mason jar, put the jar in my sab, scrape a tad bit of the print into it with a sterlized scaple, replace the lid, shake, then flame my needle, then into the syringe she goes syringe.


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.


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OfflineAmanitus
Earning my chops
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Registered: 03/31/13
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Loc: Buenos Aires
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Ziran]
    #25129687 - 04/10/18 09:31 PM (5 years, 10 months ago)

Quote:

Ziran said:
I just sterlize tap water in a mason jar, put the jar in my sab, scrape a tad bit of the print into it with a sterlized scaple, replace the lid, shake, then flame my needle, then into the syringe she goes syringe.



:thumbup:

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Offlineuniversaltruths
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Registered: 04/10/18
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Amanitus] * 1
    #25129731 - 04/10/18 09:49 PM (5 years, 10 months ago)

perhaps when you flamed the needle you did not allow enough time for them to cool , or you didn't cool it by ejecting a small amount of liquid thus destroying the spores.

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OfflineLogicaL ChaosM
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: universaltruths]
    #25130005 - 04/11/18 01:32 AM (5 years, 10 months ago)

Yeah, i dont even flame my syringe anymore. Just wipe with water.

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OfflineAmanitus
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Registered: 03/31/13
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Loc: Buenos Aires
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: universaltruths]
    #25130255 - 04/11/18 06:36 AM (5 years, 10 months ago)

Quote:

universaltruths said:
perhaps when you flamed the needle you did not allow enough time for them to cool , or you didn't cool it by ejecting a small amount of liquid thus destroying the spores.




How long do you have to wait after flaming it anyway? 5 seconds?

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OfflineFractal420
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Registered: 06/21/13
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Amanitus]
    #25130302 - 04/11/18 07:16 AM (5 years, 10 months ago)

I count to 10.

I also dont just flame the tip, the whole front half of the needle becomes black after even the first flame.

So it must be pretty hot. Generally ive always heard to expel a tiny squirt after flaming. I do that once. The only time ive had an issue with inoculate being too hot, it was from heating up my MEA scalpel too much, not waiting long enough, and scooping up a piece. Then i hear a loud *Sizzle* :facepalm:

So i threw another wedge into that jar. And it was the only one that contammed right away

Mistaaaake!


PS i wouldnt say this is a "failed" PF tek. This is just an inactive grow. It happens. One time in my MS days i shot like 2 cc's of a reaaaally dark, spore-loaded syringe into each jar, and nothing happened. I believe that was spore overload. 0.5ml would have been the right amount with those. These were rye quart jars and not BRF/verm half pints but 2cc's was still way too much of that solution. I think it was my first time with with either hawk or sw

I was like "whoa, so dark!" I didnt know that using the same 2cc as a clearer syringe would just stop my grow. You only need 2 spores it seems to start....fucking?


--------------------
Dreaming of That face again.
It's bright and blue and shimmering.
Grinning wide
And comforting me with it's three warm and wild eyes.

Prying open MY third eye


Edited by Fractal420 (04/11/18 07:28 AM)

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