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OfflinePortabellaKindaGuy
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Registered: 10/01/16
Posts: 256
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Last seen: 11 months, 7 days
First agar attempt contamination ‘Cambodian spores’
    #25128819 - 04/10/18 03:39 PM (6 years, 9 months ago)

This is day 6 (Or 7? Not entirely sure..) of my first attempt at growing mycelium from spores. I’m using pastywhyte’s “easy agar tek” with Cambodian spores. Give me as much feedback as possible on what I should do/be doing. I’m under the impression I should keep growing it out then transfer a strong chunk of each mycelial growth and transfer it to a fresh agar dish and repeat till I have a strong enough growth for transfer to grain jars, am I right? What are your thoughts on what I’ve got so far?
Also a note on my dishes, I tried to put the spores on the side of the dishes where the agar was deepest so that’s where I’m expecting mycelium to grow. Heres 2 pictures of all 7 of the dishes I set up.

Agar dish A:

There’s white dots all over the surface of this dish and one where the growth where I put spores. In my inexperience I scraped spores off the foil and simply flipped the print upside down over the agar dish because I couldn’t get any on my inoculation hook. No discoloration anywhere besides on the shallow end where there’s a yellowish blotch.
Agar fish B:

There’s some kind of contaminate with a blue center growing on the opposite side of where I the whhite growth is.
Agar dish C:

There’s one big and one small blotch that are both 100% white, no discoloration.

Agar dish D:

D definitely has some bad contamination.

Agar dish E:

Looks good to me, that’s right where I put the spores..

Agar dish F:

Looks fishy to me, discolored.

Agar dish G:

Looks the best IMO, 100% white and spreading from the center.

Any information I could use would be greatly appreciated! What would you guys do at this point?

Edited by PortabellaKindaGuy (04/10/18 03:42 PM)

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Offlinezeptepi
Nomad


Registered: 10/06/17
Posts: 12
Last seen: 2 years, 9 months
Re: First agar attempt contamination ‘Cambodian spores’ [Re: PortabellaKindaGuy]
    #25135724 - 04/13/18 12:52 PM (6 years, 9 months ago)

If you want to save all the plates, I would recommend cutting out the contaminated sections asap, otherwise toss them. Then, take one solid step towards improving your sterility by evaluating your vectors of contamination.

Next time you encounter contamination, repeat.


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