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OfflineAmanitus
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Failed PF TEK - What went wrong? Please judge my PICS.
    #25124063 - 04/08/18 06:26 PM (5 years, 11 months ago)

Hello.
I've inoculated 5 PF TEK jars more than 2 weeks ago and so far there is absolutely no sign of mycelium growing.
I've also inoculated 5 others one month ago and there is no myc growing either.
So either my syringes/prints are bad or my technique sucks.
I could be using bad syringes, now i'm in the process of making new ones.
Still, i would like you guys to take a look at the pic and tell me if you appreciate something wrong in that jars.
I opened one uncolonized month old jar and the cake was a solid block.
Did i overcook them? I followed the TEK to a T in terms of ratios.I PC'ed them for 75'.





Maybe the verm is a weird one? It's medium verm but it does not look like a previous verm that i once had that was black.
The jars are the same in both pics, one photo was taken with flash and the other one without.
Thanks for your input.

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Invisiblescrantonstrangler
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Amanitus]
    #25124080 - 04/08/18 06:35 PM (5 years, 11 months ago)

did u by chance mix water with brf and then add verm?

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InvisibleZiran
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: scrantonstrangler] * 1
    #25124103 - 04/08/18 06:46 PM (5 years, 11 months ago)

:shrug:

they look fine to me prep wise. could be a bad syringe. try shaking the syringe before you inoc next time. if you still get squat its probably a non-viable syringe.


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OfflineAmanitus
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: scrantonstrangler]
    #25124883 - 04/09/18 05:03 AM (5 years, 11 months ago)

Quote:

scrantonstrangler said:
did u by chance mix water with brf and then add verm?




No, i mixed verm and water first, then added brf.

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OfflineAmanitus
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Ziran]
    #25124891 - 04/09/18 05:10 AM (5 years, 11 months ago)

Quote:

Ziran said:
:shrug:

they look fine to me prep wise. could be a bad syringe. try shaking the syringe before you inoc next time. if you still get squat its probably a non-viable syringe.




I always shake the syringes before inoculating.

If prep wise they look fine, then it must have been that syringe.

I wondered wether my prep was ok or faulty.

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OfflineFractal420
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Amanitus]
    #25124911 - 04/09/18 05:48 AM (5 years, 11 months ago)

No growth prolly means the syringe wasnt viable or you didnt use enough (or too much).

For PF cakes, 1cc should be all you need. Because if it was truly contaminated it wouldnt look like nothing happened

This actually happened to me once back in my PF days. And i thought "why waste them" so i inoculated them with a diff syringe, and all worked out perfectly

(Although when i posted about it, people thought it was weird that i inoculated twice)

:shrug:


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OfflineAmanitus
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Fractal420]
    #25125266 - 04/09/18 09:28 AM (5 years, 11 months ago)

Quote:

Fractal420 said:
No growth prolly means the syringe wasnt viable or you didnt use enough (or too much).

For PF cakes, 1cc should be all you need. Because if it was truly contaminated it wouldnt look like nothing happened




Yeah, i did not even get contams which is weird. Looks like nothing happened at all.

Quote:


This actually happened to me once back in my PF days. And i thought "why waste them" so i inoculated them with a diff syringe, and all worked out perfectly

(Although when i posted about it, people thought it was weird that i inoculated twice)

:shrug:





Haha, that is a good idea so all that hard work and verm + brf don't go to waste. The double inoculation!

I'll give it a try on some cakes and let you know.

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OfflineLtLurker
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Amanitus]
    #25125276 - 04/09/18 09:32 AM (5 years, 11 months ago)

If your gonna re-innoc them, I'd suggest re-sterilizing them again first. If you don't any contams in there will have a big head start. Like you I thought why waste. Worked out alright.

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OfflineFractal420
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: LtLurker]
    #25125304 - 04/09/18 09:44 AM (5 years, 11 months ago)

^yup.

And use a sponsor, hopefully


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OfflineLogicaL ChaosM
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Amanitus]
    #25125313 - 04/09/18 09:47 AM (5 years, 11 months ago)

When u injected your jars, did the jars feel hot to the touch? Or even slightly too warm?

U could have cooked your spores. Ive done it before.


--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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OfflineFractal420
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: LogicaL Chaos] * 1
    #25125476 - 04/09/18 10:53 AM (5 years, 11 months ago)

Wow theres a shpongle icon!? :shpongle: :mushroom: :shpongle:
Tales of the inexpressible all the way.

I think youd be more likely to not have shaken the syringe well, creating uneven amounts of spores throughout the solution. Though you def are supposed to wait till the jars are cool (i wait overnight at least)


--------------------
Dreaming of That face again.
It's bright and blue and shimmering.
Grinning wide
And comforting me with it's three warm and wild eyes.

Prying open MY third eye


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OfflineLogicaL ChaosM
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Fractal420]
    #25125500 - 04/09/18 11:03 AM (5 years, 11 months ago)

Shpongle Spores ::shpongle::mushroom2:

Not shaking it should have at least some growth. Unless the syringe was super super dilute.

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OfflineDoDecaPus
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: LogicaL Chaos] * 1
    #25125521 - 04/09/18 11:11 AM (5 years, 11 months ago)

I advise against reinoculating. BRF and verm are not that expensive. Small price to pay to eliminate a potential problem with your colonization.

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OfflineLogicaL ChaosM
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: DoDecaPus]
    #25125528 - 04/09/18 11:14 AM (5 years, 11 months ago)

Definitely agree. Best to start over and make new jars.

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OfflineFractal420
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: LogicaL Chaos]
    #25127384 - 04/10/18 01:38 AM (5 years, 11 months ago)

I did a double noc once and zero issues. People told me not to also. But it saved a whole bunch of jars. I guess if you re-sterilize especially  its fine. Not like genetics even built up in the jar. But even if so - clearly not even viable. Might as well shoot some good spores in. If you dont wanna waste a syringe or culture, thats understandable too. But my advice, totally inoculate again if theyre clean, from experience.

They look like brand new jars pretty much. And there doesnt seem to be any growth at all. Otherwise id suggest make new ones. Just speaking from experience about my own experience with this. I think at first i shot too many spores, because it was one of those syringes you couldnt shake hard enough to make it even (had been in the fridge). Now i also keep my syringes at room temp. At least they dont freeze/thaw. And in the winter/spring here too cold for many pesky contams, so my basement is like a fridge


--------------------
Dreaming of That face again.
It's bright and blue and shimmering.
Grinning wide
And comforting me with it's three warm and wild eyes.

Prying open MY third eye


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OfflineTrappinInNature
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Fractal420] * 1
    #25128454 - 04/10/18 01:16 PM (5 years, 11 months ago)

Remember when loading jars, you want it loose and fluffy.

NO packing. Except to even it out at the top.

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Invisibleelasticaltiger
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: TrappinInNature]
    #25128502 - 04/10/18 01:37 PM (5 years, 11 months ago)

How old are your spores? You said you're making syringes? How are you making them?


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InvisibleBuffGuts
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: elasticaltiger]
    #25128525 - 04/10/18 01:43 PM (5 years, 11 months ago)

Could have had worse results...........


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OfflineAmanitus
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: LogicaL Chaos]
    #25128714 - 04/10/18 03:05 PM (5 years, 11 months ago)

Quote:

LogicaL Chaos said:
When u injected your jars, did the jars feel hot to the touch? Or even slightly too warm?

U could have cooked your spores. Ive done it before.




They were not hot.

I let them cool down overnight.

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OfflineAmanitus
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Re: Failed PF TEK - What went wrong? Please judge my PICS. [Re: Fractal420]
    #25128727 - 04/10/18 03:09 PM (5 years, 11 months ago)

Quote:

Fractal420 said:
I did a double noc once and zero issues. People told me not to also. But it saved a whole bunch of jars. I guess if you re-sterilize especially  its fine. Not like genetics even built up in the jar. But even if so - clearly not even viable. Might as well shoot some good spores in. If you dont wanna waste a syringe or culture, thats understandable too. But my advice, totally inoculate again if theyre clean, from experience.

They look like brand new jars pretty much. And there doesnt seem to be any growth at all. Otherwise id suggest make new ones. Just speaking from experience about my own experience with this. I think at first i shot too many spores, because it was one of those syringes you couldnt shake hard enough to make it even (had been in the fridge). Now i also keep my syringes at room temp. At least they dont freeze/thaw. And in the winter/spring here too cold for many pesky contams, so my basement is like a fridge




I will double sterilize and double inoculate some jars just for the experience of it. I never thought that could be a possible solution.
The jars have nothing going on inside of them. No mould or mycelium.
I opened one and dumped the contents and i don't know if this is allright or not but the cake was a solid piece. It was like a block. Is that not a symptom of overcooking the substrate?

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