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Offlineesh dov
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enough spores for spawn in jar?\grain moisture
    #25123903 - 04/08/18 07:06 PM (3 years, 1 month ago)

What happens if the syringe is light on spores? Or if not enough lc was used to inoculate grain?
i believe;
spore globs are a good sign...syringes may be potent even in they are clear.
1-2 ml of solution is usually recommend per quart jar.
curent experiment; oats pc'ed without foil. in q jars w 3 sub 1\4' holes and an injection port covered in teyvek. grains seem maybe over moist but not too wet. just over 1 ml per quart jar, each jar half - 3\4 full. testing if certain fullness works better btwn recommend fullnesses. concern is for too much moisture. were the oats cooked right. were enough spores added.


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OfflineRiverdawg78
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25123963 - 04/08/18 07:33 PM (3 years, 1 month ago)

I just inoculated 7 quart jars of PE with 4ml total about 6 days ago and have seen pretty normal growth. There are still thousands of spores in that syringe. On the LC I dont know as i havent gotten into it. Shouldn't be an issue. Good luck


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Offlineesh dov
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Re: enough spores for spawn in jar?\grain moisture [Re: Riverdawg78]
    #25124822 - 04/09/18 05:14 AM (3 years, 1 month ago)

that is reassuring. i made a mistake when i said lc i meant what's in the syringe.


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Offlineverum subsequentis
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25126876 - 04/09/18 10:09 PM (3 years, 1 month ago)

spores are shockingly small. if a syringe appears to be heavy on spores than there are millions and millions of spores in it. assuming the spore syringe was made correctly and shaken well, just a drop will have lots of spores in it


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InvisibleYesum
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Re: enough spores for spawn in jar?\grain moisture [Re: verum subsequentis]
    #25127605 - 04/10/18 08:16 AM (3 years, 1 month ago)

I like syringes with less spores.

I get turned off by dark spore syringes. Lol


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InvisibleTheMadHatter420
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Re: enough spores for spawn in jar?\grain moisture [Re: Yesum]
    #25127857 - 04/10/18 11:04 AM (3 years, 1 month ago)

You only want a couple drops of spores per jar. ALL spore syringes carry bacteria and possibly mold. The more solution you put in the jar, the more bacteria you are introducing into the jar. LESS is MORE when putting spore straight to grains.


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Offlineesh dov
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25172876 - 04/29/18 07:55 AM (3 years, 18 days ago)

out of nine jars, 3 are half white. one is starting and 5 show nothing, no white or contam, one old small jar was also pc'ed and tried with a different culture and was contam right away. so this is going slowly and barely at all. i'm thinking of using a 20qt tub for the 3 jars when they finish colonizing. since they are around half way, i wonder if i should shake em or just wait.
thinking of trying over again with bird seed as it's easier to get atm. also contemplating reinoculating the remaining oat jars.  any advise or comments could help.


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Offlineesh dov
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25175748 - 04/30/18 05:10 PM (3 years, 17 days ago)

ok so 5 show something white including 3 at 60%+ and 2 under 25%, 3 show nothing. been reading other threads. won't shake. might reinoculate the last 3.
reading that re-inoculation can work but is a risk. my jars are not contam but are slow. i don't want to waste the strain if the syringe is great but if it's not even,, then i may as well reinoculate. or i could wait till 3 jars are full and birth everything white in a 20 qt. can eve do both. will update. still open to advise.


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InvisibleTheMadHatter420
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25178619 - 05/01/18 08:41 PM (3 years, 16 days ago)

If it is slow, then there is most likely bacteria in there. Get you some agar and clean up your spores on there, it will make things SOOOOOOO much easier.


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InvisibleShroomyToons
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Re: enough spores for spawn in jar?\grain moisture [Re: TheMadHatter420]
    #25178678 - 05/01/18 09:09 PM (3 years, 16 days ago)

I wouldn't put any more from your syringe into the non showing jars.

Do get some agar if you really want to succeed at this.

Or, if you just want to play, you can learn a lot by playing with a spore syringe and BRF cakes. Like grow enough for you and your friends to have a good time and learn how mushrooms act in the process.

But if you are going for bulk growing, you pretty much have to do agar. Like Mad said...:smile:


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Offlineesh dov
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Re: enough spores for spawn in jar?\grain moisture [Re: ShroomyToons]
    #25191716 - 05/08/18 05:02 PM (3 years, 9 days ago)

sab and agar were going to be a next step. for now i got a 30q box and a bag of coir. jars are still colonizing slow. when 2 are full the plan is to mix all with coir in a tub tek. next go, try birdseed and same strain, aa+ and in half try dif strain same source in other half.


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InvisibleShroomyToons
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25192006 - 05/08/18 07:36 PM (3 years, 9 days ago)

I don't know what you mean. Try again?


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OfflineDingaz
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25194844 - 05/10/18 08:00 AM (3 years, 7 days ago)
Log in to view attachment

I've just inoculated my first batch of jars pf tek brf and vermiculite cakes.
Of the 4 I have sitting in my dark cupboard, 3 of them I mixed verm and brf together first then adding water opposed to the 4th which i mixed verm and brf first. I am concerned that i may have pressure cooked the 4th jar a bit too long as it looks very dry and still seems to have no growth. The first 3 dont seem to have any growth either.
Number 1 was injected a few days before 2,3 and 4 but being the first time had its imperfections.



PS. Starting a liquid culture with a 3.5-4% honey w distilled water, pressure cooked for 15 minutes and in the process of cooling overnight right now.
This is my last 2mL of spore solution I hope to do this correctly.


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InvisibleShroomyToons
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Re: enough spores for spawn in jar?\grain moisture [Re: Dingaz]
    #25194874 - 05/10/18 08:29 AM (3 years, 7 days ago)

Honey LC's are notorious for contamination.

If you only have 2 ml left, why not get some Pasty Plates together and do agar? Then you can drop a wedge of agar that is clean into PC'd water and make an LI that will give you lots of spawn?


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Offlineverum subsequentis
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Re: enough spores for spawn in jar?\grain moisture [Re: ShroomyToons]
    #25195047 - 05/10/18 10:46 AM (3 years, 7 days ago)

i agree with shroomy. if you are almost out of spores and don't want to buy more, i think dropping them into a honey lc is a horribly risky way to go. AGAR AGAR AGAR. for a few dollars and almost no work you can turn that little drip of spores into an amazing and almost unlimited source of innoculant.

your call though. just know that putting those spores to lc is absolutely not a guaranteed route to success. spores to lc is a bad idea in the first place because you almost never have perfectly clean spores and anything nasty will go absolutely bananas when it hits your sugar water. you may get lucky but you have been warned:hereyougo:


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Offlineesh dov
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Re: enough spores for spawn in jar?\grain moisture [Re: verum subsequentis]
    #25214960 - 05/19/18 06:22 PM (2 years, 11 months ago)

so we can inoculate some agar dishes and reproduce our spores, maybe even regain vigor that way?


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Offlineverum subsequentis
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25214975 - 05/19/18 06:29 PM (2 years, 11 months ago)

not too sure what you mean by regain vigor. drop or streak some spores on, wait for growth, transfer small portion of the cleanest looking leading edge to new plate(s), continue transferring until you have a clean culture and transfer a small portion to a new plate and put the rest to grain. once the grain is colonized you'll spawn to bulk and grow it out. you'll then be able to take more spores and or clones from your fruits and you'll still have the original culture on agar so either way you won't be needing to buy a new syringe.


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Offlineesh dov
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25214980 - 05/19/18 06:33 PM (2 years, 11 months ago)

can the mycelium from the jar be used for agar? can the syringe fluid be used for agar? ...yes.. which is better?
got 2 jars at 90% +3 jars around 50%. starting to see condensation and anxiety so going to bith into a 30q box with coir.


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Offlineesh dov
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Re: enough spores for spawn in jar?\grain moisture [Re: verum subsequentis]
    #25214981 - 05/19/18 06:35 PM (2 years, 11 months ago)

ok so wait for shrooms to do agar?
edit* oh do both ..


Edited by esh dov (05/19/18 06:37 PM)


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Offlineesh dov
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Re: enough spores for spawn in jar?\grain moisture [Re: esh dov]
    #25214984 - 05/19/18 06:40 PM (2 years, 11 months ago)

should mycelium be taken from jar or box for agar?


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Mushrooms, Mycology and Psychedelics >> Getting Started

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