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 DrMushroom
Human Farmer
Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 3 months, 24 days
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 How would i go about making a pure/clean natto (bacillus subtilis) culture 
#25122292 - 04/08/18 04:12 AM (3 years, 1 month ago) |
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Is it possible to exploit the fact natto bacteria bacillus subtilis has an extremely high tolerance for heat?
I suspect that due to where they are stored, the only source of store bought natto i can get is unsuitable for use. While the natto itself may be clean, the container is likely not, as i keep getting the exact same contamination, a sour-alcohol smelling bacteria that is present though not dominating the natto, it smells like rancid vegetable liquids, like you would get in the bottom of a food scraps bucket (for anyone that has chickens or pigs youd know this one).
Anyway, i dont like the idea of starting with dirty microbes and wishing for the best, how would i go about making a clean culture, perhaps exploting the autoclave resistance of natto endospores, or the live microbes generally high heat tolerance?
Or other methods, i dont know how bacteria cleaning on agar works
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falcon
In the green
Registered: 04/01/02
Posts: 7,614
Last seen: 4 hours, 41 minutes
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Re: How would i go about making a pure/clean natto (bacillus subtilis) culture [Re: DrMushroom]
#25124112 - 04/08/18 08:51 PM (3 years, 1 month ago) |
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Weird, I haven't tried to isolate a culture, but I've used store bought natto a lot of times for making natto, and have never had a contaminate. Even after sometimes three generations away from the store bought.
Are you buying it frozen? That's how I usually buy it.
It's very resistant to heat, sometimes I'll add the store bought, about a teaspoon, to the beans two or three cup usually, I've cooked and haven't cooled them much, probably in the 150 to 160F range.
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DrMushroom
Human Farmer
Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 3 months, 24 days
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Re: How would i go about making a pure/clean natto (bacillus subtilis) culture [Re: falcon]
#25126313 - 04/09/18 07:03 PM (3 years, 1 month ago) |
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my last grow failed since while the beans were sterile, they werent cooked, which apparently means that other bacteria can roll in.
Im going to try 1 more time, this time using another recipe/method/tek, before i look at going full sterility on this.
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