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InvisibleLemgrub
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Registered: 04/02/18
Posts: 624
Re: Cultivation General Discussion [Re: Leftfield420]
    #25118104 - 04/06/18 11:07 AM (5 years, 10 months ago)

Last night at work I had a hankering for spore knowledge of the agaricales and found a pretty good review article. It didn't give me the info I wanted but I thought someone here may find it interesting.

http://www.mycosphere.org/pdf/Mycosphere_6_1_10.pdf

Apparently spores from Psilocybe mutans can survive 9 years (p.95 paragraph 4). There are many factors that contribute to spore germination, light/lack of light, heat or cold shock, amyloidity, and others that likely don't apply to cube spores.

The amyloid coat in particular has me interested. I've heard people on here mention adding water to the agar plate after inoculating spore solution. Does anyone know if Pislocybe spores carry an amyloid coat?

Other than that I've been trying to find research I could possibly apply to cubes, which the closest relative I can find with extensive research is Coprinopsis cinerea. It's a model organism for basidiomycetes' meiosis and mating type studies. If I find anything genetically interesting I'll try to compare it to whatever cubensis genome they have on NCBI.

Anyway I'm drunk and need to go to bed.


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Offlinetryptkaloids
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I'm a teapot


Registered: 02/08/15
Posts: 12,649
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Re: Cultivation General Discussion [Re: Lemgrub]
    #25118142 - 04/06/18 11:26 AM (5 years, 10 months ago)

With the insane genetic diversity of fungi you arent going to be able to apply knowledge of one organism to another. Also, a lot of what you just posted is,over the head of many of us here. And I've been sober almost a week.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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InvisibleMateja
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Registered: 07/14/16
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25118639 - 04/06/18 03:09 PM (5 years, 10 months ago)

I´d like all the knowledgeable growers to chime in I need to know before I start doing some experiments.
The poll to be as correct as possible I´d like only those that feel fairly comfortable answering to take the poll.
If you´re not 100% sure but are pretty sure that it is in a certain range then go ahead and chime in please. Thanks guys :heart::takingnotes:
Does fruit size determine number of strains present?
You may choose only one
How many strains does one MS (multi strain) fruit contain on average
You may choose only one
Where goes the line between a clone and a limited MS culture? in terms of number of strains present.
You may choose only one


Votes accepted from (04/06/18 06:06 AM) to (No end specified)
View the results of this poll | Filter by response



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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
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Re: Cultivation General Discussion [Re: Mateja]
    #25118652 - 04/06/18 03:12 PM (5 years, 10 months ago)

No
No one knows, no one has counted
You would need a way to count strains. Basically impossible without expensive genetic testing

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InvisibleLemgrub
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Registered: 04/02/18
Posts: 624
Re: Cultivation General Discussion [Re: bodhisatta]
    #25118670 - 04/06/18 03:17 PM (5 years, 10 months ago)

Quote:

tryptkaloids said:
With the insane genetic diversity of fungi you arent going to be able to apply knowledge of one organism to another. Also, a lot of what you just posted is,over the head of many of us here. And I've been sober almost a week.




Being eukaryotes, they shouldn't differ by as much as you would think. And in the meiosis and mating type studies researchers have found that genes encoding for these processes are highly conserved across species.

I've learned so much from many of you here while I didn't post. I guess I just want to be able to give back in some way. Since I still suck too much at cultivation to innovate, figured I'd stick with what I know.


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We getting that pirate bay, alien shishkebab cordycep money

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InvisibleMateja
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Registered: 07/14/16
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25119040 - 04/06/18 05:18 PM (5 years, 10 months ago)

Quote:

bodhisatta said:
No
No one knows, no one has counted
You would need a way to count strains. Basically impossible without expensive genetic testing



Sometimes when I see narrowed down MS genetics the crops remind me more of a clone.
I guess the more strains involved the more variety in the tub and the fewer strains involved equal more uniform fruiting.
And between a MS grow with huge variety and an isolated mono culture is just a spectrum I guess..


--------------------
Cakes inside Water Tub

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Offlinetryptkaloids
Learner
I'm a teapot


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Re: Cultivation General Discussion [Re: Mateja]
    #25119207 - 04/06/18 06:10 PM (5 years, 10 months ago)

Who would have though less genetic variety would bring consitency?... :facepalm:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25119210 - 04/06/18 06:13 PM (5 years, 10 months ago)

:grin:

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InvisibleLemgrub
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Posts: 624
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25119413 - 04/06/18 07:34 PM (5 years, 10 months ago)



Can anyone confirm germination or are they messin with me. PE variety. Also a pic of leading edge myc for the fuck of it.


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InvisibleInocuole
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Re: Cultivation General Discussion [Re: Mateja]
    #25119825 - 04/06/18 11:52 PM (5 years, 10 months ago)

Quote:

Mateah said:
Quote:

bodhisatta said:
No
No one knows, no one has counted
You would need a way to count strains. Basically impossible without expensive genetic testing



Sometimes when I see narrowed down MS genetics the crops remind me more of a clone.
I guess the more strains involved the more variety in the tub and the fewer strains involved equal more uniform fruiting.
And between a MS grow with huge variety and an isolated mono culture is just a spectrum I guess..




Putting the pieces together out loud are we?  :lol:


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflineTight Lunchbox
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Re: Cultivation General Discussion [Re: Failboat]
    #25119849 - 04/07/18 12:23 AM (5 years, 10 months ago)

lol you don't feel like me. I use the xacto blades over and over and over and over again.


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InvisibleMateja
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Re: Cultivation General Discussion [Re: Inocuole]
    #25120003 - 04/07/18 04:42 AM (5 years, 10 months ago)

Quote:

Inocuole said:
Quote:

Mateah said:
Quote:

bodhisatta said:
No
No one knows, no one has counted
You would need a way to count strains. Basically impossible without expensive genetic testing



Sometimes when I see narrowed down MS genetics the crops remind me more of a clone.
I guess the more strains involved the more variety in the tub and the fewer strains involved equal more uniform fruiting.
And between a MS grow with huge variety and an isolated mono culture is just a spectrum I guess..




Putting the pieces together out loud are we?  :lol:



Yes it was very unfortunate how I formulated this dilemma :laugh2::lmafo: so the answer is "we don´t know" and now I know that :takingnotes::eatingout:


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Offlinesangreen
Dunce
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Registered: 03/31/18
Posts: 182
Loc: Enceladus
Last seen: 1 year, 2 months
Re: Cultivation General Discussion [Re: Mateja]
    #25121576 - 04/07/18 06:53 PM (5 years, 10 months ago)

I made some Pasty plates but dripped directly from syringe (spore and DIY liquid culture) to agar instead of using a loop or swab. Guess I should have read a bit more, haha, but if this works, I'm not gonna complain. Next time, I'll probably use tools, but I got my nervousness about working with agar and SABs out of the way- I'm not sure why, but it's kinda exciting working in an SAB.


--------------------
I figured it out, what life is about
It crawled into my brain and it's starting to sprout

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OfflineThufir_Hawat
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I'm a teapot

Registered: 03/18/17
Posts: 399
Last seen: 27 days, 13 hours
Re: Cultivation General Discussion [Re: sangreen]
    #25121580 - 04/07/18 06:56 PM (5 years, 10 months ago)

No worries sangreen, if there are viable spores they should still germinate! Just make sure to transfer healthy mycellium from it next time. :smile::thumbup:

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OfflineMinkMan
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Registered: 03/16/18
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Re: Cultivation General Discussion [Re: Thufir_Hawat]
    #25121587 - 04/07/18 06:59 PM (5 years, 10 months ago)

Hey guys this is my first grow. Do these jars look fully colonized and ready to be spawned into my coir vermiculite mix?


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Offlinesangreen
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Re: Cultivation General Discussion [Re: Thufir_Hawat]
    #25121589 - 04/07/18 06:59 PM (5 years, 10 months ago)

Quote:

Thufir_Hawat said:
No worries sangreen, if there are viable spores they should still germinate! Just make sure to transfer healthy mycellium from it next time. :smile::thumbup:



Yep, I'm expecting either growth full of contams (lol, worst case scenario) or wonky off-center growth. Nothing ventured, nothing gained- every mistake is a learning experience.


--------------------
I figured it out, what life is about
It crawled into my brain and it's starting to sprout

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OfflineO0ZE
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Re: Cultivation General Discussion [Re: Failboat]
    #25121613 - 04/07/18 07:11 PM (5 years, 10 months ago)

Quote:

Quirkmeister92 said:

Finally atrrived today. I feel like legit meow, like one of ya'll  :awesome:




Lol,

when pinning occurs anybody ever bottom water with a syringe and needle? i used a syringe filter, .22um, to filter some bottled water. then i switched needles and stuck it right into my cake straight through to the bottom. besides that i'm screwed because i had the lid cracked and then somebody i lived with inadvertently cranked the heat in my grow area way up. that resulted in some cake shrinkage witch primed the tub for side pin city. no biggy really, but yea. i'll chalk it up to experience. also had a jar fail.

that was weird. don't know why it failed. i feel like i let the grain get too dry after cooking it because i used a fan before pressure cooking. the others are doing fine. i took a chance with adding water to some jars that aren't quite fully colonized, but are on the dry side. we'll see what happens. I'm fine with the risk. water was also filtered with the syringe filter and needles were switched.

here is the filter chart.

Edited by O0ZE (04/07/18 07:47 PM)

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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
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Re: Cultivation General Discussion [Re: O0ZE]
    #25121759 - 04/07/18 08:09 PM (5 years, 10 months ago)

I have bottom watered.

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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: O0ZE]
    #25121777 - 04/07/18 08:18 PM (5 years, 10 months ago)

Quote:

MinkMan said:
Hey guys this is my first grow. Do these jars look fully colonized and ready to be spawned into my coir vermiculite mix?





Those don't look healthy enough to spawn. Top fruit bro
Quote:

O0ZE said:
Quote:

Quirkmeister92 said:

Finally atrrived today. I feel like legit meow, like one of ya'll  :awesome:




Lol,

when pinning occurs anybody ever bottom water with a syringe and needle? i used a syringe filter, .22um, to filter some bottled water. then i switched needles and stuck it right into my cake straight through to the bottom. besides that i'm screwed because i had the lid cracked and then somebody i lived with inadvertently cranked the heat in my grow area way up. that resulted in some cake shrinkage witch primed the tub for side pin city. no biggy really, but yea. i'll chalk it up to experience. also had a jar fail.

that was weird. don't know why it failed. i feel like i let the grain get too dry after cooking it because i used a fan before pressure cooking. the others are doing fine. i took a chance with adding water to some jars that aren't quite fully colonized, but are on the dry side. we'll see what happens. I'm fine with the risk. water was also filtered with the syringe filter and needles were switched.

here is the filter chart.



were you using agar? There's no need to filter water at all, let alone post full colonisation


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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OfflineO0ZE
Ascended Drone for the hive
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Registered: 02/28/18
Posts: 321
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25122564 - 04/08/18 07:53 AM (5 years, 10 months ago)

agar

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