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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Grow log B+ my 1st clone.
    #25118887 - 04/06/18 04:28 PM (6 years, 9 months ago)

Quote:

jaigurudeva said:


I then took a B+ clone that grew on agar and transferred it out 3 times, ending up with this. 



I have been using Pastywhite's no pour tek in glass 1/2 pint jars with SFDs instead of lids.  A little cumbersome, but I am getting used to it and haven't had many contams show on my agar dishes while the myc is growing out. 

I used Bod's EAF Grain tek and messed it up the fist time and they were too wet to begin with.  This time I started with 7 Oat jars that I prepped much drier than my first attempt, before I put the grain in jars I put my strainer over a fan, stirring occasionally, until the husks of the grain were dry. 



Then I PC'd them for two hours at 15-17 PSI, never below 15.
Here they are a few days after being inoculated with that and a couple more agar wedges in a SAB. 



Fast forward about a week and a half and this is what I'm working with:



From what I have seen, the clone I'm working with is pretty aggressive and takes things over more quickly than all of the other B+ myc I have been working with (all came from a buddy's LC that seems pretty questionable at this point with the amount of contams I've dealt with. 

Yesterday, I prepared a brick of coir.  I used Bod's bucket tek with a similar cooler to keep things hot.  I used 2.25 quarts of boiling water, as I was going for the drier side of field capacity.  This seems to have worked very well, the coir has a good moisture content without being overly moist, which I have also messed up in the past by putting 3 or 4 quarts of water and not squeezing it out...wayyyy to wet IMO, the myc had a hard time that time.

Once the coir cooled down I shook my jars and emptied them into 3 unmodified containers.  One is a shoebox and two are clear 20qt Sterlite containers.  I used a 1:1 ratio in hopes of fast colonization.  They are now in a closet with 12 on 12 off lighting with the lids on but not locked down.



It has been less than 12 hours and I already see recovery on the grains





Just moving this post from my journal so other people can chime in.

Thanks,

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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25120689 - 04/07/18 12:26 PM (6 years, 9 months ago)

Pretty happy with the recovery so far, 36 hours in:


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OfflineShrooms4life
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Registered: 06/08/17
Posts: 1,376
Loc: Under the Viel Flag
Last seen: 30 days, 17 hours
Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25120758 - 04/07/18 12:52 PM (6 years, 9 months ago)

Looking good brother keep us posted I can't wait to see your results

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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: Shrooms4life]
    #25126437 - 04/09/18 05:48 PM (6 years, 9 months ago)

Day 4 after spawn update:

Tub 1


Tub 2


Shoebox

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OfflineShrooms4life
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Registered: 06/08/17
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Last seen: 30 days, 17 hours
Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25126589 - 04/09/18 06:37 PM (6 years, 9 months ago)

Looking on track!

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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: Shrooms4life]
    #25127034 - 04/09/18 09:12 PM (6 years, 9 months ago)

Definitely colonizing a lot faster than all of my previous failed attempts.  Not sure if it is the clone, drier grains and coir, or a combo.  My first attempts were pretty damn wet...live and learn.

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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25127167 - 04/09/18 10:21 PM (6 years, 9 months ago)

I just read comebackkid's post about surface conditions and decided to do a very fine mist over the top to add just a touch of moisture to the mix.  Got some small water droplets now.

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OfflineShrooms4life
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Registered: 06/08/17
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Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25127220 - 04/09/18 10:54 PM (6 years, 9 months ago)

Yeah that's a great thread but just remember you want those beads to dry up before you add more beads.

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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: Shrooms4life]
    #25127234 - 04/09/18 11:10 PM (6 years, 9 months ago)

:thumbup:

Just a super fine mist. Now I'm going to leave them alone for a few days.

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OfflineUnsungHero
Endure & Persist


Registered: 11/21/17
Posts: 495
Loc: root@remotehost
Last seen: 11 months, 17 days
Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25127299 - 04/10/18 12:00 AM (6 years, 9 months ago)

Good luck bruh!

Keep up the FAE.

Don't let it dry out.

Handle it like a boss!

:barbershreds:


--------------------
"The quieter you become, the more you are able to hear."

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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: UnsungHero]
    #25129299 - 04/10/18 06:55 PM (6 years, 9 months ago)

All tubs are looking  close to 100% colonization at this point and the sides are just about filled in, but there are still some areas that need some growth.  I still have tiny water droplets all over the myc.     

I think I am going to wait until I see some pins to flip the lids for FAE this time.

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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25133765 - 04/12/18 05:39 PM (6 years, 9 months ago)

So here we are on day 7.  I think I will put these into fruiting conditions today by flipping my lids.  I know I said I was going to wait, but it seems like evaporation is a key pinning trigger and it looks like things are just about at 100% colonization.  Any objections or thoughts on that?  How do things look to you guys?

Tub 1:


Tub 2:


Shoebox: 


Thanks!

Actually, I think I will do a side by side comparison.  I'm going to let one tub consolidate for a few more days.  The other tub I am going to open up now.  I think I will try about a 1/2 inch casing for the shoebox then leave it shut for a few days to at least partially colonize the casing.


Edited by jaigurudeva (04/12/18 09:04 PM)

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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25141237 - 04/15/18 02:05 PM (6 years, 8 months ago)

Here is the one I put into fruiting conditions.


And the one with the lid still closed.

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Offlinejaigurudeva
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Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25153875 - 04/20/18 05:19 PM (6 years, 8 months ago)

Update day 15:

My shoebox is starting to peek through the top layer of coir I put on at 100%.  Looking good so far.

Tub 1 (put into fruiting conditions 8 days ago)


Tub 2 (put into fruiting yesterday)



What do you guys think, do they look all right?

Thanks

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Offlinejaigurudeva
Noob


Registered: 10/22/17
Posts: 62
Last seen: 1 year, 10 months
Re: Grow log B+ my 1st clone. [Re: jaigurudeva]
    #25162744 - 04/24/18 04:21 PM (6 years, 8 months ago)

Update Day 19:

I definitely have some bruising and major side pinning going on.  I still need some work on surface conditions, but this is my first batch to make it this far.     

Tub 1:  The one I put into fruiting conditions earlier.





Tub 2:  The one I put into fruiting conditions about a week later.



The shoebox, which I cased with about 1/4 inch of coir.  Which has the biggest fruit so far!  But it's on the side, haha.




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