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Invisiblesandman420
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How are commercial PE syringes made? * 1
    #25113828 - 04/04/18 02:11 PM (5 years, 9 months ago)

Does anyone have any info on how commercial syringes for strains that don't print are made, like PE and APE?


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InvisibleJHOVA
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Re: How are commercial PE syringes made? [Re: sandman420]
    #25113909 - 04/04/18 03:03 PM (5 years, 9 months ago)

put a sterilized swab into a sterilized jar with a ship and ge port. Aspirate that. Wanna be fancy add a drop of glycerin.
I think munchauzen was gonna make a video on making syringes if i recall correctly


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InvisibleGerms
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Re: How are commercial PE syringes made? [Re: JHOVA] * 2
    #25113921 - 04/04/18 03:10 PM (5 years, 9 months ago)

With a centrifuge is how Sporeworks does it I believe


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InvisibleLemurLemur
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Re: How are commercial PE syringes made? [Re: JHOVA]
    #25114006 - 04/04/18 03:51 PM (5 years, 9 months ago)

I wouldnt use a lid with a ge port since your gonna want to shake tf out of it.


Id use a long needle to suck em up after they settle, so use like a haft pint so it can reach.


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InvisiblebodhisattaMDiscordReddit
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Re: How are commercial PE syringes made? [Re: sandman420] * 3
    #25114466 - 04/04/18 07:32 PM (5 years, 9 months ago)

Quote:

Workman said:
It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.




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Invisibleelasticaltiger
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Re: How are commercial PE syringes made? [Re: bodhisatta] * 1
    #25114528 - 04/04/18 08:04 PM (5 years, 9 months ago)

Quote:

bodhisatta said:
Quote:

Workman said:
It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.







Thankyou for digging this up, I had not read that before. I always assumed they came from the occaisional "normal" looking fruits that pe sometimes puts out.


--------------------
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No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

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OfflineMr.Kaption
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Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25114537 - 04/04/18 08:08 PM (5 years, 9 months ago)

Quote:

bodhisatta said:
Quote:

Workman said:
It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.







:takingnotes:


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Invisiblejohn_conner

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Re: How are commercial PE syringes made? [Re: Mr.Kaption]
    #25114615 - 04/04/18 08:43 PM (5 years, 9 months ago)

for the DIY people with a 3d printer like myself i found this to be pretty interesting and might have to give it a go.

http://designawards.core77.com/Open-Design/62397/3D-Printed-Centrifuge-for-International-Health-Labs


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OfflineAsuraS
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Re: How are commercial PE syringes made? [Re: john_conner] * 1
    #25114621 - 04/04/18 08:47 PM (5 years, 9 months ago)

Quote:

john_conner said:
for the DIY people with a 3d printer like myself i found this to be pretty interesting and might have to give it a go.

http://designawards.core77.com/Open-Design/62397/3D-Printed-Centrifuge-for-International-Health-Labs




Salad spinner centrifuge :lol: Pretty damn clever.


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Invisiblesandman420
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Re: How are commercial PE syringes made? [Re: Asura]
    #25114627 - 04/04/18 08:52 PM (5 years, 9 months ago)

OK that is interesting about the centrifuge. So they take totally dried caps and crush them into some water and put that mixture into a centrifuge tube? What is the wetting agent, jetdry? I wonder how much water to crushed caps goes in the centrifuge tubes.


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InvisibleLemurLemur
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Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25114640 - 04/04/18 09:03 PM (5 years, 9 months ago)

Hu! How clean can it get spore solution??


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InvisiblebodhisattaMDiscordReddit
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Re: How are commercial PE syringes made? [Re: sandman420] * 1
    #25114646 - 04/04/18 09:08 PM (5 years, 9 months ago)

could be anything there's lots of wetting agents, you can get anything you desire.
dish soap is one example of one. so is jet dry.

doesn't matter how much water. it's getting removed later anyway, use enough or more.

theoretically the PE spores should be cleanest lol


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Invisiblesandman420
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Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25115244 - 04/05/18 07:05 AM (5 years, 9 months ago)

I'm guessing that the crushed caps and water mixture can't be much more than just "moistened" or it wouldn't stay separated very well after the centrifuge.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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InvisiblebodhisattaMDiscordReddit
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Re: How are commercial PE syringes made? [Re: sandman420]
    #25115271 - 04/05/18 07:15 AM (5 years, 9 months ago)

Ever used a centrifuge? The compaction keeps the layers separate


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Invisibleelasticaltiger
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Re: How are commercial PE syringes made? [Re: bodhisatta] * 1
    #25115329 - 04/05/18 07:57 AM (5 years, 9 months ago)

Yeah nothing stays together in a centrefuge.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Invisiblefilthyknees
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Re: How are commercial PE syringes made? [Re: elasticaltiger]
    #25115389 - 04/05/18 08:40 AM (5 years, 9 months ago)

Didn't someone once post using sterile water syringes to squirt the spores strait from the fresh cap into a container?

I heard centrifuge as well but do wonder if they used dried caps or if that is too much material.


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Invisiblesandman420
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Re: How are commercial PE syringes made? [Re: filthyknees]
    #25115400 - 04/05/18 08:45 AM (5 years, 9 months ago)

No, never used a centrifuge but I see there are some really cheap ones available. Do you think the little $50 4000rpm units would do the job?


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25115429 - 04/05/18 09:02 AM (5 years, 9 months ago)

Quote:

john_conner said:
for the DIY people with a 3d printer like myself i found this to be pretty interesting and might have to give it a go.

http://designawards.core77.com/Open-Design/62397/3D-Printed-Centrifuge-for-International-Health-Labs




For those of us without a 3-D printer...



How do y'all think the spores are dried? Just remove the supernatant and the non-spore bits and leave in the centrifuge tube to dry under a flowhood? Similar to an RNA pellet?

I'd like to try this with my next rounds of prints.
Any info on size and Gs needed from workman bod?

I would imagine spinning in 50 mL falcon tubes would be more efficient (It would be a PITA to add the crushed caps to 1.5 mL tubes). but I know some centrifuge inserts have a max rpm of like 4200...

Curious how easy the washing/removing the cap material is from the spore pellet. Also I wonder spore number per uL after centrifugation (or by weight of the pellet...) Sorry rambling now!


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25146195 - 04/17/18 05:51 PM (5 years, 9 months ago)

Quote:

bodhisatta said:
Quote:

Workman said:
It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.








Has anyone tried this at home? I would love to see this outcome, maybe i see new project in my future.


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OfflineWorkmanV
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Re: How are commercial PE syringes made? [Re: john_conner] * 4
    #25151287 - 04/19/18 05:11 PM (5 years, 9 months ago)

I mentioned this before, but since it isn't in my original post, it often doesn't get added.

You don't put the chunks of mushroom tissue in the centrifuge. You strain out the large pieces with a screen (paint strainer works nicely) adding additional water to wash off any clinging spores. Allow the spore laden wash water to settle for 10 minutes or so (clear glass vessel recommended) and carefully pour off the top water until you see you are starting to lose spores, then add the remaining solution to the tubes for spinning.


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