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OfflineFishLevelMidnight
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Registered: 09/01/17
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25292610 - 06/26/18 08:28 AM (5 years, 6 months ago)

How'd the cotton work for you?

Did you just put it in the water, then suck the water into the syringe through the cotton (eliminating some of the larger chunks)?

I might try this next time (I'm going to grind up like 5 g of B+ caps and do this a little more legit, make a bunch of small-medium syringes for giveaway) and sterilize the cotton before hand.

If things work out and I get germ on my plates, I am decapitating all my dried APE and seeing what I can get out of them. I want for crosses and to run generations/trades/giveawats and to see some albino spores, so I'll post a microscope pic later.


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OfflineJADO
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Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25292667 - 06/26/18 09:03 AM (5 years, 6 months ago)

I'm probably talking out my arse here but could you not just rig up a small battery drill to act as a centrifuge? Most people these days have a drill at home for diy, then all you would need is maybe some threaded steel bar a few nuts and some kind of adapter like john_conners to hold the vials in place while spinning. I know it's not really set and forget but it could work, assuming the rpm is high enough.

Edit- Found this online, this looks a bit fancier than what I was imagining.


Edited by JADO (06/26/18 09:07 AM)


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: JADO]
    #25293798 - 06/26/18 08:50 PM (5 years, 6 months ago)

Fishermansjc-for filtering the slop of spores and mushroom debris it worked great. I haven't ran a test all the way through yet, I finally have my printer up and running properly so I'm focused on printng out all the centrifuge parts now. Once its done I will run a test all the way through.

Jado- I owe burbles a dremel fuge once I'm done printing my contraption I will print a few of those out, let me know if you want one.

Burbles- didn't forget about you, like I said once I'm done mine I will print you out that dremel fuge


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Offlinek5hd2y
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25294257 - 06/27/18 05:29 AM (5 years, 6 months ago)

Quote:

john_conner said:
Fishermansjc-for filtering the slop of spores and mushroom debris it worked great. I haven't ran a test all the way through yet, I finally have my printer up and running properly so I'm focused on printng out all the centrifuge parts now. Once its done I will run a test all the way through.

Jado- I owe burbles a dremel fuge once I'm done printing my contraption I will print a few of those out, let me know if you want one.

Burbles- didn't forget about you, like I said once I'm done mine I will print you out that dremel fuge




John, I'm totally interested too man! when/if you get the time. :syringe::rocket:


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OfflineJADO
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25295598 - 06/27/18 06:10 PM (5 years, 6 months ago)

Quote:

john_conner said:
Fishermansjc-for filtering the slop of spores and mushroom debris it worked great. I haven't ran a test all the way through yet, I finally have my printer up and running properly so I'm focused on printng out all the centrifuge parts now. Once its done I will run a test all the way through.

Jado- I owe burbles a dremel fuge once I'm done printing my contraption I will print a few of those out, let me know if you want one.

Burbles- didn't forget about you, like I said once I'm done mine I will print you out that dremel fuge




It'd be wasted on me, I've no plans to grow PE just yet but thanks for the offer :thumbup:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: JADO]
    #25303924 - 07/02/18 06:59 AM (5 years, 6 months ago)

Just an update:



Almost finished here and I can finally do a test run all the way through






After I'm done this I will start on the dremel fudge for you guys

:cheers:


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Invisibleelasticaltiger
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25304090 - 07/02/18 08:52 AM (5 years, 6 months ago)

Quote:

john_conner said:
Just an update:



Almost finished here and I can finally do a test run all the way through






After I'm done this I will start on the dremel fudge for you guys

:cheers:




Jesus.  :thedude:


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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: elasticaltiger]
    #25304112 - 07/02/18 09:00 AM (5 years, 6 months ago)

An update on my end.

Of the 2 plates I tried with the spores taken from dried caps (2X at 160F actually) one got super bacterial and the other is showing no growth at all. I think the bacteria came in from shaking the plate later on and not from the syringe, as the other plate shows nothing at all.

I'll follow it a bit further; however, it seems that spores dried at 160F are not viable. I plan to dry some batches of fruit at a lower temp to see if I can keep the integrity of the spores but also dry the fruits properly. I was thinking 100F to start, any suggestions?


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25305010 - 07/02/18 05:35 PM (5 years, 6 months ago)

fishermansjc great work and thanks for the info! how did you wash the spore between each cycle? i was trying to think of the best way to do this.

I finally fished my centrifuge today hope to get a test run in asap.






Still some polishing up to do but you get the point.

Now i will start on the dremel fuges i promised and may go again at the hard drive centrifuge


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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25305273 - 07/02/18 08:06 PM (5 years, 6 months ago)

After collecting them the first time through the 22 uM filter I spun them down. Then I used PCed water to wash them (I dumped the supernatant, then added 20 mL, I was doing this in a 50 mL centrifuge tube) by vortexing to break up the pellet. Then I spun down and removed super and repeated 3X. Then added 25 mL and drew up into the two syringes I posted.

Anyone know the thermal tolerance of cubensis spores?


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25307867 - 07/04/18 09:07 AM (5 years, 6 months ago)

okay people I finished the dremel fuges there are two different kinds here. There is one that will go into a drill chuck and one that goes to a dremel.

They are up for grabs, of course k5hd2yS and BurblesV get first dibs, but after that let me know who wants one. I printed four and two are spoken for, so simple math two are left.

I will cover shipping but all i ask for in return is that, if someone can find me some PE spores i would really appreciate it, but if not then no biggie.




Disclaimer:
I have not tested these items out, proceeded at your own risk. I am not responsible for any injury that may happen. Use caution, safety glasses and common sense please.


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Invisiblesandman420
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Re: How are commercial PE syringes made? [Re: john_conner] * 1
    #25308470 - 07/04/18 03:05 PM (5 years, 6 months ago)

I bet if you keep it under 110 on the dehydrator you will be good.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: sandman420]
    #25310361 - 07/05/18 05:33 PM (5 years, 6 months ago)

So i lost all of my emails so who ever wanted a dremel fuge will have to re email me because i dont remember who asked for them.
Sorry people


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OfflineBry Bry
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25310368 - 07/05/18 05:37 PM (5 years, 6 months ago)

They look good brother!


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Bry Bry]
    #25317377 - 07/09/18 07:20 PM (5 years, 6 months ago)

bry bry - thanks!

Did i quick test run today i wanted to see how lazy you can be with these things. All work was done in the open no flow hood, SAB or gloves, cleaned twice and ran for 3 min per cycle hopefully get them on agar tomorrow to see results!

had a handful of Martinique that went for too long so i just put the caps in distilled water and coarsely filtered through cheese cloth. I let it sit for an hour or so to let the spores settle to the bottom so i could get denser amount in each tube.



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Invisiblefilthyknees
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25317648 - 07/09/18 10:28 PM (5 years, 6 months ago)

Very cool john.


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InvisibleJHOVA
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Re: How are commercial PE syringes made? [Re: filthyknees]
    #25317677 - 07/09/18 10:52 PM (5 years, 6 months ago)

Good luck john. Lets see how clean they come out.


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Offlinek5hd2y
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25320373 - 07/11/18 10:25 AM (5 years, 6 months ago)

keep the updates coming:thumbup:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: k5hd2y]
    #25327468 - 07/15/18 11:02 AM (5 years, 6 months ago)

Well the first test run went pretty much as expected, all contaimed out, kind of knew that would happen but hey sometimes you get lucky.
:mushroom:

I ran into another issue, i believe my hepa module (fake flowhood) is a cause of alot of contaims, Ive been having massive issues latley, lost 5 out of 6 shoe boxes. the odd thing is that when i do agar dishes with the hepa module they come out fine, so i have to fine tune some things to figure out whats going on. Unless i can figure out how to do it in a SAB, which the centrifuge defeats the purpose of a SAB, Im kind of SOL, Im still going to try it though.:shrug:

:mushroom2:

There are still three dremel fuges left up for grabs, Two drill chuck versions and one dremel version. :pm: if you would like one.



Heres the agar dishes with the first five spores syrgines that i ran




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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: john_conner]
    #25364899 - 08/03/18 05:22 PM (5 years, 5 months ago)

okay people im checking out for awhile good luck to everyone

cya


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